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Fatty acid composition and lipogenic enzyme protein expression in subcutaneous adipose tissue of male pigs vaccinated against boar taint, barrows, and entire boars (2013)
Journal Article

Objectives of this study were to compare fatty acid composition of subcutaneous adipose tissue of entire boars, barrows, and male pigs vaccinated against boar taint with a vaccine containing a GnRH analogue-protein conjugate (Improvac, Pfizer Animal... Read More about Fatty acid composition and lipogenic enzyme protein expression in subcutaneous adipose tissue of male pigs vaccinated against boar taint, barrows, and entire boars.

Investigation of Testosterone, Androstenone, and Estradiol Metabolism in HepG2 Cells and Primary Culture Pig Hepatocytes and Their Effects on 17βHSD7 Gene Expression (2012)
Journal Article

Steroid metabolism is important in various species. The accumulation of androgen metabolite, androstenone, in pig adipose tissue is negatively associated with pork flavor, odour and makes the meat unfit for human consumption. The 17β-hydroxysteroid d... Read More about Investigation of Testosterone, Androstenone, and Estradiol Metabolism in HepG2 Cells and Primary Culture Pig Hepatocytes and Their Effects on 17βHSD7 Gene Expression.

Influence of oilseed supplement ranging in n-6/n-3 ratio on fatty acid composition and δ5-, δ6-desaturase protein expression in steer muscles (2012)
Journal Article

This study investigated effects of roasted or extruded oilseed supplementation ranging in n-6/n-3 ratios from 0.3 to 5.0 on the fatty acid composition and expression of delta-5 desaturase (δ5d) and δ6-desaturase (δ6d) protein in commercial steer chee... Read More about Influence of oilseed supplement ranging in n-6/n-3 ratio on fatty acid composition and δ5-, δ6-desaturase protein expression in steer muscles.

Expression of the hepatic skatole- and androstenone-metabolising enzymes in entire male pigs of two live weights (2012)
Journal Article

The aim of the present study was to investigate an in vivo relationship between increased levels of testicular steroids and protein expression of the key enzymes involved in hepatic metabolism of skatole (cytochrome P4502E1 and CYP2E1 and CYP2A) and... Read More about Expression of the hepatic skatole- and androstenone-metabolising enzymes in entire male pigs of two live weights.

Effect of dietary fatty acids on expression of lipogenic enzymes and fatty acid profile in tissues of bulls (2010)
Journal Article
Martin, J., Herdmann, A., Nuernberg, K., Martin, K., Nuernberg, G., & Doran, O. (2010). Effect of dietary fatty acids on expression of lipogenic enzymes and fatty acid profile in tissues of bulls. animal, 4(5), 755-762. https://doi.org/10.1017/S1751731110000431

This study investigated the effects of dietary linolenic acid (C18:3n-3) v. linoleic acid (C18:2n-6) on fatty acid composition and protein expression of key lipogenic enzymes, acetyl-CoA carboxylase (ACC), stearoyl-CoA desaturase (SCD) and delta 6 de... Read More about Effect of dietary fatty acids on expression of lipogenic enzymes and fatty acid profile in tissues of bulls.

Comparison of two derivatisation methods for conjugated linoleic acid isomer analysis by Ag+-HPLC/DAD in beef fat (2010)
Journal Article
Dance, L. J. E., Doran, O., Hallett, K., Dannenberger, D., Nuernberg, G., & Nuernberg, K. (2010). Comparison of two derivatisation methods for conjugated linoleic acid isomer analysis by Ag+-HPLC/DAD in beef fat. European Journal of Lipid Science and Technology, 112(2), 188-194. https://doi.org/10.1002/ejlt.200900052

Two different procedures for the methylation of conjugated linoleic acid isomers (CLA) were compared by silver ion (Ag+)-HPLC. The base-catalysed method followed by an acid-catalysed method (NaOCH3/ BF3), and base hydrolysis followed by methylation w... Read More about Comparison of two derivatisation methods for conjugated linoleic acid isomer analysis by Ag+-HPLC/DAD in beef fat.

Relationship between the expression of key lipogenic enzymes, fatty acid composition, and intramuscular fat content of Limousin and Aberdeen Angus cattle (2010)
Journal Article
Ward, R. E., Woodward, B., Otter, N., & Doran, O. (2010). Relationship between the expression of key lipogenic enzymes, fatty acid composition, and intramuscular fat content of Limousin and Aberdeen Angus cattle. Livestock Science, 127(1), 22-29. https://doi.org/10.1016/j.livsci.2009.09.005

The amount and fatty acid composition of intramuscular fat (IMF) are major factors in determining the nutritional value of beef. Understanding the mechanisms regulating IMF formation is important for designing strategies for manipulation of IMF quant... Read More about Relationship between the expression of key lipogenic enzymes, fatty acid composition, and intramuscular fat content of Limousin and Aberdeen Angus cattle.

Acetyl-CoA carboxylase and stearoyl-CoA desaturase protein expression in subcutaneouse adipose tissue is reduced in pigs selected for decreased backfat thickness at constant intramuscular fat content (2009)
Journal Article
Canovas, A., Estany, J., Tor, M., Pena, R., & Doran, O. (2009). Acetyl-CoA carboxylase and stearoyl-CoA desaturase protein expression in subcutaneouse adipose tissue is reduced in pigs selected for decreased backfat thickness at constant intramuscular fat content. Journal of Animal Science, 87(12), 3905-3914. https://doi.org/10.2527/jas.2009-2091

Acetyl-CoA carboxylase and stearoyl-CoA desaturase protein expression in subcutaneous adipose tissue is reduced in pigs selected for decreased backfat thickness at constant intramuscular fat content (2009)
Journal Article
Cánovas, A., Estany, J., Tor, M., Pena, R. N., & Doran, O. (2009). Acetyl-CoA carboxylase and stearoyl-CoA desaturase protein expression in subcutaneous adipose tissue is reduced in pigs selected for decreased backfat thickness at constant intramuscular fat content. Journal of Animal Science, 87(12), 3905-3914. https://doi.org/10.2527/jas.2009-2091

The objectives of this study were 1) to determine whether selection toward less subcutaneous fat thickness at constant intramuscular fat content in pigs is related to tissue-specific changes in the expression of lipogenic enzymes acetyl-CoA carboxyla... Read More about Acetyl-CoA carboxylase and stearoyl-CoA desaturase protein expression in subcutaneous adipose tissue is reduced in pigs selected for decreased backfat thickness at constant intramuscular fat content.

Effect of breed on fatty acid composition and stearoyl-CoA desaturase protein expression in the Semimembranosus muscle and subcutaneous adipose tissue of cattle (2009)
Journal Article
Dance, L. J., Matthews, K. R., & Doran, O. (2009). Effect of breed on fatty acid composition and stearoyl-CoA desaturase protein expression in the Semimembranosus muscle and subcutaneous adipose tissue of cattle. Livestock Science, 125(2-3), 291-297. https://doi.org/10.1016/j.livsci.2009.05.009

The present study investigated (i) the effect of breed on the expression of stearoyl-CoA desaturase (SCD) protein and fatty acid composition in Semimembranosus muscle and subcutaneous adipose tissue of beef cattle and (ii) the relationship between SC... Read More about Effect of breed on fatty acid composition and stearoyl-CoA desaturase protein expression in the Semimembranosus muscle and subcutaneous adipose tissue of cattle.

Effect of supplementation of the maternal diet with fish oil or linseed oil on fatty-acid composition and expression of δ5- and δ6-desaturase in tissues of female piglets (2009)
Journal Article
Missotten, J., De Smet, S., Raes, K., & Doran, O. (2009). Effect of supplementation of the maternal diet with fish oil or linseed oil on fatty-acid composition and expression of δ5- and δ6-desaturase in tissues of female piglets. animal, 3(8), 1196-1204. https://doi.org/10.1017/S1751731109004455

The present study investigated whether enrichment of the pig maternal diet with n-3 polyunsaturated fatty acids (PUFA) affects the fatty-acid composition of female piglets via enhancing of expression of the lipogenic enzymes 5-desaturase (5d) and 6-d... Read More about Effect of supplementation of the maternal diet with fish oil or linseed oil on fatty-acid composition and expression of δ5- and δ6-desaturase in tissues of female piglets.

Delta 9-desaturase protein expression and fatty acid composition of longissimus dorsi muscle in lambs fed green herbage or concentrate with or without added tannins (2009)
Journal Article
Vasta, V., Priolo, A., Scerra, M., Hallett, K., Wood, J., & Doran, O. (2009). Delta 9-desaturase protein expression and fatty acid composition of longissimus dorsi muscle in lambs fed green herbage or concentrate with or without added tannins. Meat Science, 82(3), 357-364. https://doi.org/10.1016/j.meatsci.2009.02.007

Δ 9 desaturase protein expression and fatty acid composition of longissimus dorsi muscle in lambs fed green herbage or concentrate with or without added tannins (2009)
Journal Article
Wood, J. D., Hallett, K. G., Vasta, V., Priolo, A., Scerra, M., & Doran, O. (2009). Δ 9 desaturase protein expression and fatty acid composition of longissimus dorsi muscle in lambs fed green herbage or concentrate with or without added tannins. Meat Science, 82(3), 357-364. https://doi.org/10.1016/j.meatsci.2009.02.007

The aims of this study were to investigate the effect of feeding system and of supplementation of tannins (8.93% DM) on the relationship between intramuscular fat content, fatty acid composition and Δ 9 desaturase (Δ 9 d) protein expression in longis... Read More about Δ 9 desaturase protein expression and fatty acid composition of longissimus dorsi muscle in lambs fed green herbage or concentrate with or without added tannins.

Effect of sex steroids on expression of sulfotransferase 2B1 immunoreactive protein in primary cultured porcine hepatocytes (2008)
Journal Article
Panella-Riera, N., Moe, M., Grindflek, E., Oliver, M. A., Wood, J. D., & Doran, O. (2008). Effect of sex steroids on expression of sulfotransferase 2B1 immunoreactive protein in primary cultured porcine hepatocytes. Livestock Science, 118(3), 223-230. https://doi.org/10.1016/j.livsci.2008.01.021

An excessive accumulation of androstenone (5α-androst-16-en-3-one) in pig adipose tissue is one of the two major contributors to the phenomenon of boar taint. High levels of adipose tissue androstenone have been related to a low rate of hepatic andro... Read More about Effect of sex steroids on expression of sulfotransferase 2B1 immunoreactive protein in primary cultured porcine hepatocytes.

Effect of testosterone, estrone sulphate and androstenone on 3β-hydroxysteroid dehydrogenase protein expression in primary cultured hepatocytes (2008)
Journal Article
Nicolau-Solano, S. I., & Doran, O. (2008). Effect of testosterone, estrone sulphate and androstenone on 3β-hydroxysteroid dehydrogenase protein expression in primary cultured hepatocytes. Livestock Science, 114(2-3), 202-210. https://doi.org/10.1016/j.livsci.2007.05.001

Androstenone (5α-androst-16-en-3-one) is a steroid pheromone, excessive accumulation of which in pig adipose tissue contributes to the phenomenon of "boar taint". The incidence of boar taint increases with age and weight of entire male pigs. One of t... Read More about Effect of testosterone, estrone sulphate and androstenone on 3β-hydroxysteroid dehydrogenase protein expression in primary cultured hepatocytes.

Regulation of CYP2A6 protein expression by skatole, indole, and testicular steroids in primary cultured pig hepatocytes (2008)
Journal Article
Wood, J. D., Chen, G., Cue, R. A., Lundstrom, K., & Doran, O. (2008). Regulation of CYP2A6 protein expression by skatole, indole, and testicular steroids in primary cultured pig hepatocytes. Drug Metabolism and Disposition, 36(1), 56-60. https://doi.org/10.1124/dmd.107.017285

CYP2A6 is one of the enzymes involved in the hepatic metabolism of a naturally produced compound, skatole, in the pig. Low CYP2A6 activity has been linked to excessive accumulation of skatole in pig adipose tissue and development of the phenomenon "b... Read More about Regulation of CYP2A6 protein expression by skatole, indole, and testicular steroids in primary cultured pig hepatocytes.

Expression of 3β-hydroxysteroid dehydrogenase, cytochrome P450-c17, and sulfotransferase 2B1 proteins in liver and testis of pigs of two breeds: Relationship with adipose tissue androstenone concentration (2007)
Journal Article
Moe, M., Moe, E., Grindflek, E., & Doran (nee Udovikova), O. (2007). Expression of 3β-hydroxysteroid dehydrogenase, cytochrome P450-c17, and sulfotransferase 2B1 proteins in liver and testis of pigs of two breeds: Relationship with adipose tissue androstenone concentration. Journal of Animal Science, 85(11), 2924-2931. https://doi.org/10.2527/jas.2007-0283

An excessive accumulation of androstenone in pig adipose tissue is a major contributor to the phenomenon of boar taint. Androstenone deposition is dependent on the rate of androstenone biosynthesis in testis and androstenone degradation in liver. The... Read More about Expression of 3β-hydroxysteroid dehydrogenase, cytochrome P450-c17, and sulfotransferase 2B1 proteins in liver and testis of pigs of two breeds: Relationship with adipose tissue androstenone concentration.

Relationship between carcass weight, adipose tissue androstenone level and expression of the hepatic 3β-hydroxysteroid dehydrogenase in entire commercial pigs (2007)
Journal Article
Nicolau-Solano, S. I., Wittington, F. M., Wood, J. D., & Doran (nee Udovikova), O. (2007). Relationship between carcass weight, adipose tissue androstenone level and expression of the hepatic 3β-hydroxysteroid dehydrogenase in entire commercial pigs. animal, 1(7), 1053-1059. https://doi.org/10.1017/S1751731107000274

Boar taint is a major meat-quality defect in pigs and is due to excessive accumulation of skatole and androstenone in adipose tissue. The present work investigated the relationship between carcass weight, levels of skatole and androstenone in adipose... Read More about Relationship between carcass weight, adipose tissue androstenone level and expression of the hepatic 3β-hydroxysteroid dehydrogenase in entire commercial pigs.