Identification of a peroxide-sensitive redox switch at the CXXC motif in the human mitochondrial branched chain aminotransferase

Conway, Myra E.; Yennawar, Neela; Wallin, Reidar; Poole, Leslie B.; Hutson, Susan M.

doi:10.1021/bi020200i

All Outputs (3)

Crystal structures of human mitochondrial branched chain aminotransferase reaction intermediates: Ketimine and pyridoxamine phosphate forms (2002)
Journal Article

The three-dimensional structures of the isoleucine ketimine and the pyridoxamine phosphate forms of human mitochondrial branched chain aminotransferase (hBCATm) have been determined crystallographically at 1.9 Å resolution. The hBCATm-catalyzed trans... Read More about Crystal structures of human mitochondrial branched chain aminotransferase reaction intermediates: Ketimine and pyridoxamine phosphate forms.

A continuous 96-well plate spectrophotometric assay for branched-chain amino acid aminotransferases (2002)
Journal Article

A new, continuous 96-well plate spectrophotometric assay for the branched-chain amino acid aminotransferases is described. Transamination of L-leucine with α-ketoglutarate results in formation of α-ketoisocaproate, which is reductively aminated back... Read More about A continuous 96-well plate spectrophotometric assay for branched-chain amino acid aminotransferases.

Identification of a peroxide-sensitive redox switch at the CXXC motif in the human mitochondrial branched chain aminotransferase (2002)
Journal Article

The human mitochondrial branched chain aminotransferase isoenzyme (hBCATm) must be stored in a reducing environment to remain active. Oxidation or labeling of hBCATm with sulfhydryl reagents results in enzyme inhibition. In this study, we investigate... Read More about Identification of a peroxide-sensitive redox switch at the CXXC motif in the human mitochondrial branched chain aminotransferase.