All Outputs (38)

Differentiation of SH-SY5Y neuroblastoma cells using retinoic acid and BDNF: A model for neuronal and synaptic differentiation in neurodegeneration (2024)
Journal Article
Targett, I. L., Crompton, L. A., Conway, M. E., & Craig, T. J. (in press). Differentiation of SH-SY5Y neuroblastoma cells using retinoic acid and BDNF: A model for neuronal and synaptic differentiation in neurodegeneration. In Vitro Cellular and Developmental Biology - Animal,

There has been much interest in the use of cell culture models of neurones, to avoid the animal welfare and cost issues of using primary and human-induced pluripotent stem cell (hiPSC)-derived neurones respectively. The human neuroblastoma cell line,... Read More about Differentiation of SH-SY5Y neuroblastoma cells using retinoic acid and BDNF: A model for neuronal and synaptic differentiation in neurodegeneration.

Shedding smart light on the effectiveness of chemotherapy: using Raman spectroscopy and machine learning to differentiate the effects of Cytarabine toxicity and crosstalk of leukaemic and bone marrow stromal cells (2021)
Journal Article
Gynn, L., Lamb-Riddell, K., Cox, T., Hansen, M., Conway, M., & May, J. (2021). Shedding smart light on the effectiveness of chemotherapy: using Raman spectroscopy and machine learning to differentiate the effects of Cytarabine toxicity and crosstalk of leukaemic and bone marrow stromal cells. British Journal of Haematology, 193(S1), 46-47

Mesenchymal stromal cells (MSC) protect leukaemic cells from drug-induced toxicity within the bone marrow niche, with increasing evidence of leukaemic impact on supportive stroma. The nucleoside analogue, cytarabine (ara-C), is a front-line agent for... Read More about Shedding smart light on the effectiveness of chemotherapy: using Raman spectroscopy and machine learning to differentiate the effects of Cytarabine toxicity and crosstalk of leukaemic and bone marrow stromal cells.

BCATc modulates crosstalk between the PI3K/Akt and the Ras/ERK pathway regulating proliferation in triple negative breast cancer (2020)
Journal Article
Shafei, M. A., Forshaw, T., Davis, J., Flemban, A., Qualtrough, D., Dean, S., …Conway, M. E. (2020). BCATc modulates crosstalk between the PI3K/Akt and the Ras/ERK pathway regulating proliferation in triple negative breast cancer. Oncotarget, 11, 1971-1987. https://doi.org/10.18632/oncotarget.27607

The cytosolic branched chain aminotransferase (BCATc) protein has been found to be highly expressed in breast cancer subtypes, including triple negative breast cancer (TNBC), compared with normal breast tissue. The catabolism of branched-chain amino... Read More about BCATc modulates crosstalk between the PI3K/Akt and the Ras/ERK pathway regulating proliferation in triple negative breast cancer.

BCAT-induced autophagy regulates Aβ load through an interdependence of redox state and PKC phosphorylation-implications in Alzheimer's disease (2020)
Journal Article
Harris, M., El Hindy, M., Usmari-Moraes, M., Hudd, F., Shafei, M., Dong, M., …Conway, M. E. (in press). BCAT-induced autophagy regulates Aβ load through an interdependence of redox state and PKC phosphorylation-implications in Alzheimer's disease. Free Radical Biology and Medicine, https://doi.org/10.1016/j.freeradbiomed.2020.01.019

Leucine, nutrient signal and substrate for the branched chain aminotransferase (BCAT) activates the mechanistic target of rapamycin (mTORC1) and regulates autophagic flux, mechanisms implicated in the pathogenesis of neurodegenerative conditions such... Read More about BCAT-induced autophagy regulates Aβ load through an interdependence of redox state and PKC phosphorylation-implications in Alzheimer's disease.

Novel blood biomarkers that correlate with cognitive performance and hippocampal volumetry: Potential for early diagnosis of Alzheimer's disease (2019)
Journal Article

© 2019 - IOS Press and the authors. All rights reserved. Background: Differential diagnosis of people presenting with mild cognitive impairment (MCI) that will progress to Alzheimer's disease (AD) remains clinically challenging. Current criteria used... Read More about Novel blood biomarkers that correlate with cognitive performance and hippocampal volumetry: Potential for early diagnosis of Alzheimer's disease.

Immunotherapeutic and pharmacological approaches for the treatment of Alzheimer’s disease (2018)
Journal Article

The development of efficacious treatments targeting Alzheimer’s disease (AD) aetiology has been proved an extensive, time consuming task. Currently prescribed therapies, primarily acetylcholinesterase inhibitors such as donepezil and the NMDAreceptor... Read More about Immunotherapeutic and pharmacological approaches for the treatment of Alzheimer’s disease.

Hypoxia modulates the stem cell population and induces EMT in the MCF-10A breast epithelial cell line (2017)
Journal Article

A common feature among pre-malignant lesions is the induction of hypoxia through increased cell propagation and reduced access to blood flow. Hypoxia in breast cancer has been associated with poor patient prognosis, resistance to chemotherapy and inc... Read More about Hypoxia modulates the stem cell population and induces EMT in the MCF-10A breast epithelial cell line.

Distribution of the branched-chain α-ketoacid dehydrogenase complex E1α subunit and glutamate dehydrogenase in the human brain and their role in neuro-metabolism (2017)
Journal Article

© 2017 Glutamate is the major excitatory neurotransmitter of the central nervous system, with the branched-chain amino acids (BCAAs) acting as key nitrogen donors for de novo glutamate synthesis. Despite the importance of these major metabolites, the... Read More about Distribution of the branched-chain α-ketoacid dehydrogenase complex E1α subunit and glutamate dehydrogenase in the human brain and their role in neuro-metabolism.

The impact of ageing reveals distinct roles for human dentate gyrus and CA3 in pattern separation and object recognition memory (2017)
Journal Article

© 2017 The Author(s). Both recognition of familiar objects and pattern separation, a process that orthogonalises overlapping events, are critical for effective memory. Evidence is emerging that human pattern separation requires dentate gyrus. Dentate... Read More about The impact of ageing reveals distinct roles for human dentate gyrus and CA3 in pattern separation and object recognition memory.

Evaluation of recombinant factor C assay for the detection of divergent lipopolysaccharide structural species and comparison with Limulus amebocyte lysate-based assays and a human monocyte activity assay (2017)
Journal Article

© 2017 The Authors. Purpose. The Limulus amebocytelysate (LAL) assay is widely used for the screening of lipopolysaccharide (LPS) in parenteral pharmaceuticals. However, correlation of LPS in Gram-negative bacterial infections by LAL assay has been p... Read More about Evaluation of recombinant factor C assay for the detection of divergent lipopolysaccharide structural species and comparison with Limulus amebocyte lysate-based assays and a human monocyte activity assay.

Divergent metabolic regulation of autophagy and mTORC1-early events in Alzheimer's disease? (2017)
Journal Article

© 2017 Shafei, Harris and Conway. Alzheimer's disease (AD) is a progressive disease associated with the production and deposition of amyloid β-peptide (Aβ) aggregates and neurofibrillary tangles, which lead to synaptic and neuronal damage. Reduced au... Read More about Divergent metabolic regulation of autophagy and mTORC1-early events in Alzheimer's disease?.

Enhanced task-related brain activation and resting perfusion in healthy older adults after chronic blueberry supplementation (2017)
Journal Article

© 2017, Canadian Science Publishing. All rights reserved. Blueberries are rich in flavonoids, which possess antioxidant and anti-inflammatory properties. High flavonoid intakes attenuate age-related cognitive decline, but data from human intervention... Read More about Enhanced task-related brain activation and resting perfusion in healthy older adults after chronic blueberry supplementation.

Decreased expression of the mitochondrial BCAT protein correlates with improved patient survival in IDH-WT gliomas (2016)
Journal Article
Ellis, H. P., Williams, M., Hull, J., Conway, M. E., Hull, J., El Hindy, M., …Kurian, K. M. (2016). Decreased expression of the mitochondrial BCAT protein correlates with improved patient survival in IDH-WT gliomas. Brain Pathology, 26(6), 789-791. https://doi.org/10.1111/bpa.12385

Background and research question: Gliomas represent 43% of all solid intracranial tumours, of which glioblastomas have the poorest prognosis. Recently, the human cytosolic branched-chain aminotransferase protein (hBCATc), which metabolises the branch... Read More about Decreased expression of the mitochondrial BCAT protein correlates with improved patient survival in IDH-WT gliomas.

Altered Expression of Human Mitochondrial Branched Chain Aminotransferase in Dementia with Lewy Bodies and Vascular Dementia (2016)
Journal Article

© 2016, The Author(s). Cytosolic and mitochondrial human branched chain aminotransferase (hBCATc and hBCATm, respectively) play an integral role in brain glutamate metabolism. Regional increased levels of hBCATc in the CA1 and CA4 region of Alzheimer... Read More about Altered Expression of Human Mitochondrial Branched Chain Aminotransferase in Dementia with Lewy Bodies and Vascular Dementia.

New insights into the role of the branched-chain aminotransferase proteins in the human brain (2015)
Journal Article

© 2015 Wiley Periodicals, Inc. The human cytosolic branched-chain aminotransferase (hBCATc) enzyme is strategically located in glutamatergic neurons, where it is thought to provide approximately 30% of de novo nitrogen for brain glutamate synthesis.... Read More about New insights into the role of the branched-chain aminotransferase proteins in the human brain.

Regional Increase in the Expression of the BCAT Proteins in Alzheimer's Disease Brain: Implications in Glutamate Toxicity (2015)
Journal Article

© 2015 - IOS Press and the authors. All rights reserved. Background: The human branched chain aminotransferases (hBCATm, mitochondrial and hBCATc, cytosolic) are major contributors to brain glutamate production. This excitatory neurotransmitter is th... Read More about Regional Increase in the Expression of the BCAT Proteins in Alzheimer's Disease Brain: Implications in Glutamate Toxicity.

S-nitrosylation of the thioredoxin-like domains of protein disulfide isomerase and its role in neurodegenerative conditions (2015)
Journal Article

© 2015 Conway and Harris. Correct protein folding and inhibition of protein aggregation is facilitated by a cellular "quality control system" that engages a network of protein interactions including molecular chaperones and the ubiquitin proteasome s... Read More about S-nitrosylation of the thioredoxin-like domains of protein disulfide isomerase and its role in neurodegenerative conditions.

The redox switch that regulates molecular chaperones (2015)
Journal Article

© 2015 by De Gruyter. Modification of reactive cysteine residues plays an integral role in redox-regulated reactions. Oxidation of thiolate anions to sulphenic acid can result in disulphide bond formation, or overoxidation to sulphonic acid, represen... Read More about The redox switch that regulates molecular chaperones.

The branched-chain aminotransferase proteins: Novel redox chaperones for protein disulfide isomerase-implications in Alzheimer's disease (2014)
Journal Article

Aims: The human branched-chain aminotransferase proteins (hBCATm and hBCATc) are regulated through oxidation and S-nitrosation. However, it remains unknown whether they share common redox characteristics to enzymes such as protein disulfide isomerase... Read More about The branched-chain aminotransferase proteins: Novel redox chaperones for protein disulfide isomerase-implications in Alzheimer's disease.

Investigation and verification of a bioluminescent biosensor for the quantitation of ara-CTP generation: A biomarker for cytosine arabinoside sensitivity in acute myeloid leukaemia (2014)
Journal Article

A novel whole cell bacterial biosensor, which emits light in response to the active metabolite of cytosine arabinoside (ara-C, cytarabine), ara-CTP, has been investigated and verified. The biosensor has been formulated as an ex vivo assay, designed f... Read More about Investigation and verification of a bioluminescent biosensor for the quantitation of ara-CTP generation: A biomarker for cytosine arabinoside sensitivity in acute myeloid leukaemia.