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The challenges of the analysis of basic compounds by high performance liquid chromatography: Some possible approaches for improved separations

McCalley, David V.

Authors



Abstract

This review considers some of the difficulties encountered with the analysis of ionised bases using reversed-phase chromatography, such as detrimental interaction with column silanol groups, and overloading which both lead to poor peak shapes. Methods of overcoming these problems in reversed-phase (RP) separations, by judicious selection of the column and mobile phase conditions, are discussed. Hydrophilic interaction chromatography is considered as an alternative method for the separation of some basic compounds. © 2009 Elsevier B.V. All rights reserved.

Citation

McCalley, D. V. (2010). The challenges of the analysis of basic compounds by high performance liquid chromatography: Some possible approaches for improved separations. Journal of Chromatography A, 1217(6), 858-880. https://doi.org/10.1016/j.chroma.2009.11.068

Journal Article Type Review
Publication Date Feb 5, 2010
Journal Journal of Chromatography A
Print ISSN 0021-9673
Publisher Elsevier
Peer Reviewed Not Peer Reviewed
Volume 1217
Issue 6
Pages 858-880
DOI https://doi.org/10.1016/j.chroma.2009.11.068
Keywords pharmaceuticals, biomedically significant compounds, HPLC
basic compounds, HPLC, stationary phases, reversed-phase
HILIC
Public URL https://uwe-repository.worktribe.com/output/981379
Publisher URL http://dx.doi.org/10.1016/j.chroma.2009.11.068