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Evaluation of inflammatory effects of airborne endotoxin emitted from composting sources

Pankhurst, Louise J.; Deacon, Lewis J.; Tyrrel, Sean F.; Hayes, Enda T; Drew, Gillian H; Liu, Jian; Longhurst, Philip; Abate, Wondwossen; Pollard, Simon; Longhurst, James; Jackson, Simon K.


Louise J. Pankhurst

Lewis J. Deacon

Sean F. Tyrrel

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Enda Hayes
Professor in Air Quality and Carbon Management

Gillian H Drew

Jian Liu

Philip Longhurst

Wondwossen Abate

Simon Pollard

Simon K. Jackson


Because of the lack of effective methodology, the biological effects of environmental endotoxin have not been assessed. Here we have collected and measured airborne endotoxin at different locations around composting sites. Increased endotoxin concentrations were observed close to composting activities and also at nearby boundary areas. Analysis of proinflammatory effects of the environmental endotoxin on interleukin (IL)-8 and IL-6 release from human D562 pharyngeal epithelial and MM6 monocytic cell cultures showed an association between endotoxin level and cytokine induction. The cytokine-inducing effect of bioaerosol extracts was inhibited by polymyxin B, indicating that endotoxin was the cause of cytokine responses we found. The environmental endotoxin was also more active for stimulating cytokines in airway epithelial cells than commercially purified Escherichia coli endotoxin. Our results suggest that these in vitro inflammatory cell models may contribute to the assessment of health impacts of environmental endotoxin. Environ. Toxicol. Chem. 2011; 30:602-606. © 2011 SETAC Copyright © 2011 SETAC.

Journal Article Type Article
Publication Date Mar 1, 2011
Journal Environmental Toxicology and Chemistry
Print ISSN 0730-7268
Electronic ISSN 1552-8618
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 30
Issue 3
Pages 602-606
APA6 Citation Tyrrel, S. F., Longhurst, P. J., Drew, G. H., Hayes, E. T., Deacon, L. J., Pankhurst, L. J., …Jackson, S. K. (2011). Evaluation of inflammatory effects of airborne endotoxin emitted from composting sources. Environmental Toxicology and Chemistry, 30(3), 602-606.
Keywords endotoxin, composting, in vitro, cytokine
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