Tatsuo Miyamoto
Insufficiency of BUBR1, a mitotic spindle checkpoint regulator, causes impaired ciliogenesis in vertebrates
Miyamoto, Tatsuo; Porazinski, Sean; Wang, Huijia; Borovina, Antonia; Ciruna, Brian; Shimizu, Atsushi; Kajii, Tadashi; Kikuchi, Akira; Furutani-Seiki, Makoto; Matsuura, Shinya
Authors
Sean Porazinski
Huijia Wang
Antonia Borovina
Brian Ciruna
Atsushi Shimizu
Tadashi Kajii
Akira Kikuchi
Makoto Furutani-Seiki
Shinya Matsuura
Abstract
Budding uninhibited by benzimidazole-related 1 (BUBR1) is a central molecule of the spindle assembly checkpoint. Germline mutations in the budding uninhibited by benzimidazoles 1 homolog beta gene encoding BUBR1 cause premature chromatid separation (mosaic variegated aneuploidy) [PCS (MVA)] syndrome, which is characterized by constitutional aneuploidy and a high risk of childhood cancer. Patients with the syndrome often develop Dandy-Walker complex and polycystic kidneys; implying a critical role of BUBR1 in morphogenesis. However, little is known about the function of BUBR1 other than mitotic control. Here, we report that BUBR1 is essential for the primary cilium formation, and that the PCS (MVA) syndrome is thus a novel ciliopathy. Morpholino knockdown of bubr1 in medaka fish also caused ciliary dysfunction characterized by defects in cerebellar development and perturbed left-right asymmetry of the embryo. Biochemical analyses demonstrated that BUBR1 is required for ubiquitin-mediated proteasomal degradation of cell division cycle protein 20 in the G0 phase and maintains anaphase-promoting complex/cyclosome-CDC20 homolog 1 activity that regulates the optimal level of dishevelled for ciliogenesis. © The Author 2011. Published by Oxford University Press. All rights reserved.
Journal Article Type | Article |
---|---|
Acceptance Date | Feb 28, 2011 |
Publication Date | May 1, 2011 |
Journal | Human Molecular Genetics |
Print ISSN | 0964-6906 |
Electronic ISSN | 1460-2083 |
Publisher | Oxford University Press (OUP) |
Peer Reviewed | Peer Reviewed |
Volume | 20 |
Issue | 10 |
Pages | 2058-2070 |
DOI | https://doi.org/10.1093/hmg/ddr090 |
Keywords | insufficiency, BUBR1, mitotic, spindle, checkpoint, regulator, impaired, ciliogenesis, vertebrates |
Public URL | https://uwe-repository.worktribe.com/output/962621 |
Publisher URL | http://dx.doi.org/10.1093/hmg/ddr090 |
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