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Hyphenated mass spectrometry versus real‐time mass spectrometry techniques for the detection of volatile compounds from the human body

Gould, Oliver; Drabi?ska, Natalia; Ratcliffe, Norman; de Lacy Costello, Ben

Hyphenated mass spectrometry versus real‐time mass spectrometry techniques for the detection of volatile compounds from the human body Thumbnail


Authors

Natalia Drabi?ska

Norman Ratcliffe Norman.Ratcliffe@uwe.ac.uk
Professor in Materials & Sensors Science



Abstract

Mass spectrometry (MS) is an analytical technique that can be used for various applications in a number of scientific areas including environmental, security, forensic science, space exploration, agri‐food, and numerous others. MS is also continuing to offer new insights into the proteomic and metabolomic fields. MS techniques are frequently used for the analysis of volatile compounds (VCs). The detection of VCs from human samples has the potential to aid in the diagnosis of diseases, in monitoring drug metabolites, and in providing insight into metabolic processes. The broad usage of MS has resulted in numerous variations of the technique being developed over the years, which can be divided into hyphenated and real‐time MS techniques. Hyphenated chromatographic techniques coupled with MS offer unparalleled qualitative analysis and high accuracy and sensitivity, even when analysing complex matrices (breath, urine, stool, etc.). However, these benefits are traded for a significantly longer analysis time and a greater need for sample preparation and method development. On the other hand, real‐time MS techniques offer highly sensitive quantitative data. Additionally, real‐time techniques can provide results in a matter of minutes or even seconds, without altering the sample in any way. However, real‐time MS can only offer tentative qualitative data and suffers from molecular weight overlap in complex matrices. This review compares hyphenated and real‐time MS methods and provides examples of applications for each technique for the detection of VCs from humans.

Citation

Gould, O., Drabińska, N., Ratcliffe, N., & de Lacy Costello, B. (2021). Hyphenated mass spectrometry versus real‐time mass spectrometry techniques for the detection of volatile compounds from the human body. Molecules, 26(23), Article 7185. https://doi.org/10.3390/molecules26237185

Journal Article Type Review
Acceptance Date Nov 23, 2021
Online Publication Date Nov 26, 2021
Publication Date Nov 26, 2021
Deposit Date Apr 26, 2022
Publicly Available Date Mar 29, 2024
Journal Molecules
Electronic ISSN 1420-3049
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 26
Issue 23
Article Number 7185
DOI https://doi.org/10.3390/molecules26237185
Keywords Chemistry (miscellaneous); Analytical Chemistry; Organic Chemistry; Physical and Theoretical Chemistry; Molecular Medicine; Drug Discovery; Pharmaceutical Science
Public URL https://uwe-repository.worktribe.com/output/9208378

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