Dr Oliver Gould Oliver.Gould@uwe.ac.uk
Casual Demonstrator - CHSS
Hyphenated mass spectrometry versus real‐time mass spectrometry techniques for the detection of volatile compounds from the human body
Gould, Oliver; Drabi?ska, Natalia; Ratcliffe, Norman; de Lacy Costello, Ben
Authors
Natalia Drabi?ska
Norman Ratcliffe Norman.Ratcliffe@uwe.ac.uk
Professor in Materials & Sensors Science
Benjamin De Lacy Costello Ben.DeLacyCostello@uwe.ac.uk
Associate Professor in Diagnostics and Bio-Sensing Technology
Abstract
Mass spectrometry (MS) is an analytical technique that can be used for various applications in a number of scientific areas including environmental, security, forensic science, space exploration, agri‐food, and numerous others. MS is also continuing to offer new insights into the proteomic and metabolomic fields. MS techniques are frequently used for the analysis of volatile compounds (VCs). The detection of VCs from human samples has the potential to aid in the diagnosis of diseases, in monitoring drug metabolites, and in providing insight into metabolic processes. The broad usage of MS has resulted in numerous variations of the technique being developed over the years, which can be divided into hyphenated and real‐time MS techniques. Hyphenated chromatographic techniques coupled with MS offer unparalleled qualitative analysis and high accuracy and sensitivity, even when analysing complex matrices (breath, urine, stool, etc.). However, these benefits are traded for a significantly longer analysis time and a greater need for sample preparation and method development. On the other hand, real‐time MS techniques offer highly sensitive quantitative data. Additionally, real‐time techniques can provide results in a matter of minutes or even seconds, without altering the sample in any way. However, real‐time MS can only offer tentative qualitative data and suffers from molecular weight overlap in complex matrices. This review compares hyphenated and real‐time MS methods and provides examples of applications for each technique for the detection of VCs from humans.
Citation
Gould, O., Drabińska, N., Ratcliffe, N., & de Lacy Costello, B. (2021). Hyphenated mass spectrometry versus real‐time mass spectrometry techniques for the detection of volatile compounds from the human body. Molecules, 26(23), Article 7185. https://doi.org/10.3390/molecules26237185
Journal Article Type | Review |
---|---|
Acceptance Date | Nov 23, 2021 |
Online Publication Date | Nov 26, 2021 |
Publication Date | Nov 26, 2021 |
Deposit Date | Apr 26, 2022 |
Publicly Available Date | Mar 29, 2024 |
Journal | Molecules |
Electronic ISSN | 1420-3049 |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 26 |
Issue | 23 |
Article Number | 7185 |
DOI | https://doi.org/10.3390/molecules26237185 |
Keywords | Chemistry (miscellaneous); Analytical Chemistry; Organic Chemistry; Physical and Theoretical Chemistry; Molecular Medicine; Drug Discovery; Pharmaceutical Science |
Public URL | https://uwe-repository.worktribe.com/output/9208378 |
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Hyphenated mass spectrometry versus real-time mass spectrometry techniques for the detection of volatile compounds from the human body
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