Brenneria goodwinii growth in vitro is improved by competitive interactions with other bacterial species associated with Acute Oak Decline

Brady, Carrie; Orsi, Mario; Doonan, James; Denman, Sandra; Arnold, Dawn

Abstract

Brenneria goodwinii, Rahnella victoriana and Gibbsiella quercinecans are three bacterial species frequently isolated together from oak displaying symptoms of Acute Oak Decline (AOD), which include weeping patches on trunks. All three bacterial species play a role in lesion formation in the current episode of AOD in Britain, although B. goodwinii is the most dominant. The ongoing research into stem lesion formation characteristic of this polybacterial syndrome has been focussed primarily on the pathogenicity, identification and taxonomy of these bacteria. As all three species were newly classified within the past ten years, there are many unanswered questions regarding their ecology and interactions with each other. To determine the effect of bacterial interactions on fitness in vitro, we examined pairwise (diculture) and multispecies (triculture) interactions between B. goodwinii, R. victoriana and G. quercinecans in oak leaf media microcosms. Additionally, the effect of co-culturing on the evolution of these species was determined and the evolved B. goodwinii strains were examined further by whole genome sequencing. Our results indicate that B. goodwinii thrived in monoculture with significantly higher viable cell counts than the other two species. Additionally, B. goodwinii performed well in pairwise culture with mutually competitive interactions observed between B. goodwinii and R. victoriana, and between B. goodwinii and G. quercinecans. In the multispecies triculture, B. goodwinii and R. victoriana appeared to exhibit co-ordinated behaviour to outcompete G. quercinecans. After four weeks B. goodwinii grown in co-culture with the other two species developed greater evolved fitness than the strain grown in monoculture as reflected by the increased viable cell counts. The competitive interactions taking place between the threes species indicated evolving improved fitness of B. goodwinii in vitro, that gave it a growth advantage over both R. victoriana and G. quercinecans which showed no significant changes in fitness. Overall, B. goodwinii gains greater benefit in terms of fitness from in vitro competitive interaction with the other two species.

Citation

Brady, C., Orsi, M., Doonan, J., Denman, S., & Arnold, D. (2022). Brenneria goodwinii growth in vitro is improved by competitive interactions with other bacterial species associated with Acute Oak Decline. Current Research in Microbial Sciences, 3, Article 100102. https://doi.org/10.1016/j.crmicr.2021.100102

• Journal Article Type: Article
• Acceptance Date: Dec 17, 2021
• Online Publication Date: Dec 28, 2021
• Publication Date: 2022
• Deposit Date: Feb 25, 2022
• Publicly Available Date: Feb 25, 2022
• Journal: Current Research in Microbial Sciences
• Electronic ISSN: 2666-5174
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 3
• Article Number: 100102
• DOI: https://doi.org/10.1016/j.crmicr.2021.100102
• Public URL: https://uwe-repository.worktribe.com/output/8467124