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The impact of pressure and frictional heating on retention, selectivity and efficiency in ultra-high-pressure liquid chromatography

McCalley, David V.

Authors



Abstract

© 2014 Elsevier B.V. The effects of pressure and frictional heating deserve serious consideration in ultra-high-pressure liquid chromatography (UHPLC) separations, as the pressures used can be three times greater than those in conventional high-performance LC (HPLC). We show that the effects of pressure alone can give useful selectivity effects, especially when separating molecules of different size. Frictional heating effects can cause serious losses in column efficiency and may also give changes in the selectivity of the separation. Nevertheless, the detrimental effect of frictional heating can be reduced, for instance by the judicious selection of column thermostat and particle type. In practical situations, pressure and heating effects occur simultaneously and can cause problems in transferring methods from conventional HPLC to UHPLC. In reversed-phase separations, the typical effect of increased retention with increasing pressure is counteracted by the reduction in retention that usually occurs at elevated temperatures.

Journal Article Type Review
Publication Date Dec 1, 2014
Journal TrAC - Trends in Analytical Chemistry
Print ISSN 0165-9936
Electronic ISSN 1879-3142
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 63
Pages 31-43
APA6 Citation McCalley, D. V. (2014). The impact of pressure and frictional heating on retention, selectivity and efficiency in ultra-high-pressure liquid chromatography. Trends in Analytical Chemistry, 63, 31-43. https://doi.org/10.1016/j.trac.2014.06.024
DOI https://doi.org/10.1016/j.trac.2014.06.024
Keywords efficiency, frictional heating, heating effect. high-performance liquid chromatography, HPLC, pressure effect, retention, reversed-phase separation, selectivity, separation, ultra-high-pressure liquid chromatography, UHPLC
Publisher URL http://dx.doi.org/10.1016/j.trac.2014.06.024