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Bilirubin oxidase based enzymatic air-breathing cathode: Operation under pristine and contaminated conditions

Erable, Benjamin; Babanova, Sofia; Schuler, Andrew; Atanassov, Plamen; Santoro, Carlo

Authors

Benjamin Erable

Sofia Babanova

Andrew Schuler

Plamen Atanassov



Abstract

© 2015 Elsevier B.V. The performance of bilirubin oxidase (BOx) based air breathing cathode was constantly monitored over 45days. The effect of electrolyte composition on the cathode oxygen reduction reaction (ORR) output was investigated. Particularly, deactivation of the electrocatalytic activity of the enzyme in phosphate buffer saline (PBS) solution and in activated sludge (AS) was evaluated. The greatest drop in current density was observed during the first 3days of constant operation with a decrease of ~60μAcm-2day-1. The rate of decrease slowed to ~10μAcm-2day-1 (day 3 to 9) and then to ~1.5μAcm-2day-1 thereafter (day 9 to 45). Despite the constant decrease in output, the BOx cathode generated residual current after 45days operations with an open circuit potential (OCP) of 475mV vs. Ag/AgCl. Enzyme deactivation was also studied in AS to simulate an environment close to the real waste operation with pollutants, solid particles and bacteria. The presence of low-molecular weight soluble contaminants was identified as the main reason for an immediate enzymatic deactivation within few hours of cathode operation. The presence of solid particles and bacteria does not affect the natural degradation of the enzyme.

Journal Article Type Article
Publication Date Apr 1, 2016
Journal Bioelectrochemistry
Print ISSN 1567-5394
Electronic ISSN 1878-562X
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 108
Pages 1-7
Institution Citation Atanassov, P., Schuler, A., Erable, B., Babanova, S., & Santoro, C. (2016). Bilirubin oxidase based enzymatic air-breathing cathode: Operation under pristine and contaminated conditions. Bioelectrochemistry, 108, 1-7. https://doi.org/10.1016/j.bioelechem.2015.10.005
DOI https://doi.org/10.1016/j.bioelechem.2015.10.005
Keywords Bilirubin oxidase; Oxygen reduction reaction; Enzymes durability; PBS conditions; Pollutants
Publisher URL http://dx.doi.org/10.1016/j.bioelechem.2015.10.005