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Virulence of a T6SS Campylobacter jejuni chicken isolate from North Romania

Ungureanu, Vlad A.; Stratakos, Alexandros Ch.; Gundogdu, Ozan; Stef, Lavinia; Pet, Ioan; Pet, Elena; Pacala, Nicolae; Corcionivoschi, Nicolae

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Authors

Vlad A. Ungureanu

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Alexandros Stratakos Alexandros.Stratakos@uwe.ac.uk
Associate Professor in Sustainable Agri-Food Production

Ozan Gundogdu

Lavinia Stef

Ioan Pet

Elena Pet

Nicolae Pacala

Nicolae Corcionivoschi



Abstract

Objectives: In this study we have investigated the in vitro and in vivo virulence characteristics of a new T6SS positive Campylobacter jejuni chicken isolate (SV12) originating from a poultry population in North Romania. A detailed phenotypic characterization was performed and compared to the T6SS negative C. jejuni 81-176 wild strain. Results: Our results indicate that the significantly higher capacity to attach and invade HCT-8 cells of C. jejuni SV12 isolate is associated with increased motility, increased resistance to bile salts and serum resistance, when compared to C. jejuni strain 81-76. Mice infected with the SV12 isolate showed statistically higher levels of colonization at both 7- and 14-days post-inoculation and in the stomach, caecum, duodenum and large intestine. Infection with the SV12 strain induced a stronger immune response as the gene transcript levels of IL-17, TNFα and IFNγ were more pronouncedly up-regulated compared to the C. jejuni strain 81-176. The present study showed that the new isolate SV12 had an enhanced virulence capacity compared to the wild strain which was evident in vivo as well. This work also provides an insight on the colonization pattern and host immune response differences between T6SS positive and T6SS negative C. jejuni.

Citation

Ungureanu, V. A., Stratakos, A. C., Gundogdu, O., Stef, L., Pet, I., Pet, E., …Corcionivoschi, N. (2019). Virulence of a T6SS Campylobacter jejuni chicken isolate from North Romania. BMC Research Notes, 12(1), Article 180. https://doi.org/10.1186/s13104-019-4201-8

Journal Article Type Article
Acceptance Date Mar 16, 2019
Online Publication Date Mar 28, 2019
Publication Date Mar 28, 2019
Deposit Date Jun 25, 2021
Publicly Available Date Mar 29, 2024
Journal BMC Research Notes
Electronic ISSN 1756-0500
Publisher BioMed Central
Peer Reviewed Peer Reviewed
Volume 12
Issue 1
Article Number 180
DOI https://doi.org/10.1186/s13104-019-4201-8
Keywords General Biochemistry, Genetics and Molecular Biology; General Medicine
Public URL https://uwe-repository.worktribe.com/output/7450784
Additional Information Received: 26 November 2018; Accepted: 16 March 2019; First Online: 28 March 2019

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