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Laryngeal transplantation in minipigs: Vascular, myologic and functional outcomes

Birchall, M. A.; Kingham, P. J.; Murison, P. J.; Ayling, S. M.; Burt, R.; Mitchard, L.; Jones, A.; Lear, P.; Stokes, C. R.; Terenghi, G.; Bailey, M.; MacChiarini, P.

Authors

M. A. Birchall

P. J. Kingham

P. J. Murison

R. Burt

L. Mitchard

A. Jones

P. Lear

C. R. Stokes

G. Terenghi

M. Bailey

P. MacChiarini



Abstract

There is no effective way of replacing all the functions of the larynx in those requiring laryngectomy. Regenerative medicine offers promise, but cannot presently deliver implants with functioning neuromuscular units. A single well-documented laryngeal transplant in man was a qualified success, but more information is required before clinical trials may be proposed. We studied the early response of the larynx to laryngeal transplantation between 17 pairs of NIH minipigs full matched at the MHC2 locus. Following iterative technical improvements, pigs had good swallowing and a patent airway at 1 week. No significant changes in mucosal blood flux were observed compared with pre-operative measurements. Changes in muscle morphology and fibre phenotype were observed in transplant muscles retrieved after 7 days: the levels of fast and slow myosin heavy chain (MyHC) protein were reduced and embryonic MyHC was up regulated consistent with denervation induced atrophy. At 1 week laryngeal transplantation can result in good swallowing, and is not associated with clinical evidence of ischemia-reperfusion injury in MHC-matched pigs. © 2010 Springer-Verlag.

Citation

Birchall, M. A., Kingham, P. J., Murison, P. J., Ayling, S. M., Burt, R., Mitchard, L., …MacChiarini, P. (2011). Laryngeal transplantation in minipigs: Vascular, myologic and functional outcomes. European Archives of Oto-Rhino-Laryngology, 268(3), 405-414. https://doi.org/10.1007/s00405-010-1355-3

Journal Article Type Article
Online Publication Date Sep 15, 2010
Publication Date 2011-03
Deposit Date May 19, 2021
Journal European Archives of Oto-Rhino-Laryngology
Print ISSN 0937-4477
Electronic ISSN 1434-4726
Publisher Springer (part of Springer Nature)
Peer Reviewed Peer Reviewed
Volume 268
Issue 3
Pages 405-414
DOI https://doi.org/10.1007/s00405-010-1355-3
Keywords Otorhinolaryngology; General Medicine
Public URL https://uwe-repository.worktribe.com/output/6903111