Generation of spatio-temporal concentration profiles for cell culture systems: A case study in ammonia

Yue, Xicai; Drakakisa, Emmanuel M.; Mantalaris, Athanasios; Cass, Anthony

Abstract

Cellular behavior is influenced by micro-concentration gradients existing in their immediate spatio-environment. A practical real-time measurement system, composed of miniaturized long-lasting sensors placed within a two-dimensional bioreactor and a 32-channel data acquisition system for ammonia, is presented in this paper aiming to generate the spatio-temporal ammonia concentration profiles for the whole culture process. Our results demonstrate the feasibility of the system and its applicability in elucidating the cell growth micro-environment within a bioreactor, which can potentially facilitate the intelligent control of ammonia levels in cell culture systems. The presented system is flexible enough to interface other sensors for generating their spatio-temporal profiles of the sensed cell culture
parameters.

• Journal Article Type: Article
• Online Publication Date: Jun 10, 2010
• Publication Date: Nov 1, 2010
• Deposit Date: May 7, 2021
• Print ISSN: 1536-6367
• Electronic ISSN: 1536-6359
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 43
• Issue: 9
• Pages: 1207-1216
• DOI: https://doi.org/10.1016/j.measurement.2010.05.012
• Public URL: https://uwe-repository.worktribe.com/output/5667469