Skip to main content

Research Repository

Advanced Search

An in vitro investigation of microbial volatile analysis for diagnosis of wound infection

Slade, Elisabeth

Authors

Elisabeth Slade



Abstract

Microorganisms produce a wide range of volatile compounds as a product of metabolism. As a result of their different metabolic capabilities, the profiles of volatiles produced will differ between species. This research investigates the detection of volatile profiles from bacterial species associated with wound infection, for species discrimination when cultured under wound like conditions.
This work shows that by combing the analysis of bacterial culture headspace using Selected Ion Flow Tube-Mass Spectrometry (SIFT-MS) with multivariate statistical analysis it is possible to discriminate wound-associated bacterial species in planktonic culture, in both complex culture media and a simulated wound fluid. Furthermore, this research demonstrates the detection of volatile profiles from discarded clinical wound dressings. To advance the use of volatile analysis of wound-associated bacteria for species discrimination, a novel collagen based biofilm model providing wound-like culture conditions was developed and characterised. Use of this model for the culture of reproducible steady-state biofilms, and analysis of biofilm headspace volatile profiles was successfully undertaken and subsequently employed in combination with SIFT-MS and multivariate statistical analysis. Results show that the discrimination of wound-associated bacterial biofilms based on headspace volatile profiles under wound like conditions was feasible. In addition, SIFT-MS analysis of multispecies biofilms was undertaken and the detected headspace profiles compared to those of single species biofilms. This indicated that identification of multispecies biofilms using detection of volatile metabolites is more complex, as the headspace was dominated by volatile compounds associated with only one of the species present.
The results of this research indicate that application of volatile analysis to wound diagnostics may be of use for the identification of the cause of infection, particularly within acute wounds, which are often caused by a single species.

Citation

Slade, E. An in vitro investigation of microbial volatile analysis for diagnosis of wound infection. (Thesis). UWE. Retrieved from https://uwe-repository.worktribe.com/output/4917997

Thesis Type Thesis
Deposit Date Dec 20, 2019
Publicly Available Date Mar 28, 2024
Public URL https://uwe-repository.worktribe.com/output/4917997
Award Date Dec 19, 2019

Files

An in vitro investigation of microbial volatile analysis for diagnosis of wound infection (10.7 Mb)
PDF

Licence
http://www.rioxx.net/licenses/all-rights-reserved

Publisher Licence URL
http://www.rioxx.net/licenses/all-rights-reserved

Copyright Statement
The article 'In Vitro discrimination of wound-associated bacteria by volatile compound profiling using selected ion flow tube-mass spectrometry' is included in this thesis under the following conditions in the copyright transfer agreement:

3. Final Published Version. The Owner hereby licenses back to the Contributor the following rights with respect to the final published version of the Contribution (the "Final Published Version"):

b. Re-use in other publications. The right to re-use the Final Published Version or parts thereof for any publication authored or edited by the Contributor (excluding journal articles) where such re-used material
constitutes less than half of the total material in such publication. In such case, any modifications must be accurately noted.




You might also like



Downloadable Citations