An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells

Trakarnsanga, Kongtana; Griffiths, Rebecca E.; Wilson, Marieangela C.; Blair, Allison; Satchwell, Timothy J.; Meinders, Marjolein; Cogan, Nicola; Kupzig, Sabine; Kurita, Ryo; Nakamura, Yukio; Toye, Ashley M.; Anstee, David J.; Frayne, Jan

Abstract

With increasing worldwide demand for safe blood, there is much interest in generating red blood cells in vitro as an alternative clinical product. However, available methods for in vitro generation of red cells from adult and cord blood progenitors do not yet provide a sustainable supply, and current systems using pluripotent stem cells as progenitors do not generate viable red cells. We have taken an alternative approach, immortalizing early adult erythroblasts generating a stable line, which provides a continuous supply of red cells. The immortalized cells differentiate efficiently into mature, functional reticulocytes that can be isolated by filtration. Extensive characterization has not revealed any differences between these reticulocytes and in vitro-cultured adult reticulocytes functionally or at the molecular level, and importantly no aberrant protein expression. We demonstrate a feasible approach to the manufacture of red cells for clinical use from in vitro culture.

• Journal Article Type: Article
• Acceptance Date: Jan 26, 2017
• Online Publication Date: Mar 14, 2017
• Publication Date: Mar 14, 2017
• Deposit Date: Jul 11, 2024
• Publicly Available Date: Jul 12, 2024
• Journal: Nature Communications
• Electronic ISSN: 2041-1723
• Publisher: Nature Research (part of Springer Nature)
• Peer Reviewed: Peer Reviewed
• Volume: 8
• Article Number: 14750
• DOI: https://doi.org/10.1038/ncomms14750
• Public URL: https://uwe-repository.worktribe.com/output/12121498