Pantoea vagans sp. nov., Pantoea eucalypti sp. nov., Pantoea deleyi sp. nov. and Pantoea anthophila sp. nov

Brady, Carrie L.; Venter, Stephanus N.; Cleenwerck, Ilse; Engelbeen, Katrien; Vancanneyt, Marc; Swings, Jean; Coutinho, Teresa A.

Abstract

Bacteria isolated from eucalyptus leaves and shoots showing symptoms of blight and die-back collected in Uganda, Uruguay and Argentina and from maize displaying brown stalk rot symptoms in South Africa were tentatively placed in the genus Pantoea on the basis of phenotypic and biochemical tests. These isolates, together with two strains (LMG 2558 and LMG 2560) previously assigned to Pantoea agglomerans based on protein electrophoregrams but later excluded from this species, were further investigated using molecular techniques. 16S rRNA gene sequencing and multilocus sequence analyses (MLSA) revealed that the strains were phylogenetically closely related to Pantoea agglomerans, Pantoea stewartii and Pantoea ananatis. MLSA and amplified fragment length polymorphism analysis placed the strains into four separate clusters, not containing any of the type strains of species of the genus Pantoea. DNA-DNA hybridization confirmed the classification of the isolates into four novel species, for which the names Pantoea vagans sp. nov. (type strain R-21566T 5LMG 24199T 5BCC 105T 5BD 765T), Pantoea eucalypti sp. nov. (type strain R-25678T 5LMG 24197T 5BCC 076T 5BD 769T), Pantoea deleyi sp. nov. (type strain R-31523T 5LMG 24200T 5BCC 109T 5BD 767T) and Pantoea anthophila sp. nov. (type strain LMG 2558T 5BD 871T 5NCPPB 1682T) are proposed. © 2009 IUMS.

• Journal Article Type: Article
• Publication Date: Sep 1, 2009
• Deposit Date: Mar 22, 2024
• Journal: International Journal of Systematic and Evolutionary Microbiology
• Print ISSN: 1466-5026
• Electronic ISSN: 1466-5034
• Publisher: Microbiology Society
• Peer Reviewed: Peer Reviewed
• Volume: 59
• Issue: 9
• Pages: 2339-2345
• DOI: https://doi.org/10.1099/ijs.0.009241-0
• Public URL: https://uwe-repository.worktribe.com/output/11834039