Precursor of human adenovirus core polypeptide Mu targets the nucleolus and modulates the expression of E2 proteins

Lawrence, Fiona; Lee, T. W R; Dauksaite, V.; Akusj�rvi, G.; Blair, G. E.; Matthews, David A.

Abstract

We have examined the subcellular localization properties of human adenovirus 2 (HAdV-2) preMu and mature Mu (pX) proteins as fusions with enhanced green fluorescence protein (EGFP). We determined that preMu is exclusively a nucleolar protein with a single nucleolar accumulation signal within the Mu sequence. In addition, we noted that both preMu-EGFP and Mu-EGFP are excluded from adenovirus DNA-binding protein (DBP)-rich replication centres in adenovirus-infected cells. Surprisingly, we observed that cells in which preMu-EGFP (but not Mu-EGFP) is transiently expressed prior to or shortly after infection with Ad2 did not express late adenovirus genes. Further investigation suggested this might be due to a failure to express pre-terminal protein (preTP) from the E2 region, despite expression of another E2 protein, DBP. Deletion mutagenesis identified a highly conserved region in the C terminus of preMu responsible for these observations. Thus our data suggest that preMu may play a role in modulating accumulation of proteins from the E2 region.

• Journal Article Type: Article
• Publication Date: Jan 1, 2004
• Journal: Journal of General Virology
• Print ISSN: 0022-1317
• Electronic ISSN: 1465-2099
• Publisher: Microbiology Society
• Peer Reviewed: Peer Reviewed
• Volume: 85
• Issue: 1
• Pages: 185-196
• DOI: https://doi.org/10.1099/vir.0.19352-0
• Keywords: adenovirus, E2 proteins
• Public URL: https://uwe-repository.worktribe.com/output/1063832
• Publisher URL: http://dx.doi.org/10.1099/vir.0.19352-0