Activation marker expression and apoptotic susceptibility of T-cell clones derived from CD34+, young and SENIEUR donors

Della Valle, Nicola S.; B�rcia, Rita N.; Pawelec, Graham; McLeod, Julie D.

Abstract

T-cell clones (TCC) derived from human peripheral blood lymphocytes of a young control, a healthy elderly (SENIEUR) donor, or from CD34+ hematopoietic progenitor cells were utilised in this study to examine how in vivo and in vitro ageing affects T-cell apoptotic capability. The role of CD25, CD28 and the intracellular proteins, FLICE-inhibitory protein (FLIP), receptor-interacting protein (RIP) and caspase 3 were investigated. We observed an age-related decline in the expression of the IL-2 receptor α chain CD25, and absence of the co-stimulatory receptor CD28 on three of the four TCC studied. In young donor- and CD34 cell-derived TCC, but not in SENIEUR donor-derived TCC, we observed an age-related increase in susceptibility of the cells to mFas-L-induced apoptosis, which correlated with the age-related decrease of CD25 expression. Expression levels of full-length RIP and FLIP did not show any correlation to apoptotic susceptibility. However, expression levels of the cleaved form of RIP were greatly reduced in the SENIEUR donor-derived TCC, which together with a trend towards increased caspase 3 activity, could indicate an age-related alteration in utilisation of different apoptotic signalling pathways. © 2004 Elsevier Inc. All rights reserved.

Citation

McLeod, J. D., Pawelec, G., Bárcia, R. N., & Della Valle, N. S. (2004). Activation marker expression and apoptotic susceptibility of T-cell clones derived from CD34+, young and SENIEUR donors. Experimental Gerontology, 39(4), 531-538. https://doi.org/10.1016/j.exger.2003.11.015

• Journal Article Type: Conference Paper
• Publication Date: Apr 1, 2004
• Journal: Experimental Gerontology
• Print ISSN: 0531-5565
• Publisher: Elsevier
• Peer Reviewed: Not Peer Reviewed
• Volume: 39
• Issue: 4
• Pages: 531-538
• DOI: https://doi.org/10.1016/j.exger.2003.11.015
• Keywords: T-cell clones, CD25, apoptosis, Fas/Fas-L, receptor-interacting protein, FLICE-inhibitory protein
• Public URL: https://uwe-repository.worktribe.com/output/1062795
• Publisher URL: http://dx.doi.org/10.1016/j.exger.2003.11.015