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A novel, disposable, screen-printed amperometric biosensor for glucose in serum fabricated using a water-based carbon ink

Cowell, David C.; Pittson, Robin W.; Crouch, Eric; Hoskins, Stephen; Hart, John P.

Authors

David C. Cowell

Robin W. Pittson

Eric Crouch



Abstract

Screen-printed amperometric glucose biosensors have been fabricated using a water-based carbon ink. The enzyme glucose oxidase (GOD) and the electrocatalyst cobalt phthalocyanine were mixed with the carbon ink prior to the screen-printing process; therefore, biosensors are prepared in a one-step fabrication procedure. Optimisation of the biosensor performance was achieved by studying the effects of pH, buffer strength, and applied potential on the analytical response. Calibration studies were performed under optimum conditions, using amperometry in stirred solution, with an operating potential of +500 mV versus SCE. The sensitivity was found to be 1170 nA mM-1, with a linear range of 0.025-2 mM; the former represents the detection limit. The disposable amperometric biosensor was evaluated by carrying out replicate determinations on a sample of bovine serum. This was achieved by the method of multiple standard additions and included a correction for background currents arising from oxidisable serum components. The mean serum concentration was calculated to be 8.63 mM and compared well with the supplier's value of 8.3 mM; the coefficient of variation was calculated to be 3.3% (n = 6). © 2005 Elsevier B.V. All rights reserved.

Citation

Pittson, R. W., Cowell, D. C., Crouch, E., Hoskins, S., & Hart, J. P. (2005). A novel, disposable, screen-printed amperometric biosensor for glucose in serum fabricated using a water-based carbon ink. Biosensors and Bioelectronics, 21(5), 712-718. https://doi.org/10.1016/j.bios.2005.01.003

Journal Article Type Article
Publication Date Nov 15, 2005
Journal Biosensors and Bioelectronics
Print ISSN 0956-5663
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 21
Issue 5
Pages 712-718
DOI https://doi.org/10.1016/j.bios.2005.01.003
Keywords screen-printed, amperometric, biosensor, hydrogen peroxide, glucose, biocomposite ink
Public URL https://uwe-repository.worktribe.com/output/1046279
Publisher URL http://dx.doi.org/10.1016/j.bios.2005.01.003