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A study of retention and overloading of basic compounds with mixed-mode reversed-phase/cation-exchange columns in high performance liquid chromatography

Davies, Nicola H.; Euerby, Melvin R.; McCalley, David V.

Authors

Nicola H. Davies

Melvin R. Euerby



Abstract

The retention and overload of bases were studied on two new mixed-mode, silica based phases possessing ionic carboxylate functionalities of different acidity embedded within a hydrophobic ligand (SiELC Primesep™). At low pH, good peak shapes were obtained for small solute mass, suggesting that the mere presence of a mixed-mode hydrophobic/ionic retention mechanism is not responsible for the poor peak shape that can occur on conventional reversed-phases with ionised silanols. Somewhat inferior, but still acceptable peak shape for bases was obtained on a column containing a mixture of discrete ion exchange and reversed-phase particles (Hypersil Duet). In both types of column, the ionic sites favourably increased the capacity for ionised bases, reducing considerably the deterioration of peak shape with load observed with conventional RP columns. The combined ionic and reversed-phase interaction can give strong retention of bases under certain conditions, necessitating careful choice of stationary and mobile phase. © 2006 Elsevier B.V. All rights reserved.

Citation

Davies, N. H., Euerby, M. R., & McCalley, D. V. (2007). A study of retention and overloading of basic compounds with mixed-mode reversed-phase/cation-exchange columns in high performance liquid chromatography. Journal of Chromatography A, 1138(1-2), 65-72. https://doi.org/10.1016/j.chroma.2006.10.002

Journal Article Type Article
Publication Date Jan 5, 2007
Journal Journal of Chromatography A
Print ISSN 0021-9673
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 1138
Issue 1-2
Pages 65-72
DOI https://doi.org/10.1016/j.chroma.2006.10.002
Keywords basic drugs, HPLC, overload, mixed-mode mechanisms
Public URL https://uwe-repository.worktribe.com/output/1032449
Publisher URL http://dx.doi.org/10.1016/j.chroma.2006.10.002