Stephen Smith
A comparative study of the analysis of human urine headspace using gas chromatography-mass spectrometry
Smith, Stephen; Burden, H.; de Lacy Costello, Ben; Persad, R.; Ratcliffe, Norman M.; Whittington, K.; Probert, C. S.
Authors
H. Burden
Benjamin De Lacy Costello Ben.DeLacyCostello@uwe.ac.uk
Associate Professor in Diagnostics and Bio-Sensing Technology
R. Persad
Norman Ratcliffe Norman.Ratcliffe@uwe.ac.uk
Professor in Materials & Sensors Science
K. Whittington
C. S. Probert
Abstract
First-void urine samples were obtained from 24 elderly, asymptomatic men (median age 62.9 years). The headspace above pH adjusted urine samples were extracted using a carboxen/polydimethylsiloxane solid phase micro-extraction fibre and the volatile organic compounds analysed by gas chromatography/mass spectrometry. A total of 147 compounds were identified in the headspace of urine. The acidified samples recorded a total of 92 compounds, 27 of which were ubiquitous, basified samples 70 compounds, with 12 ubiquitous and unmodified pH samples 49, with 6 ubiquitous. Five compounds were ubiquitous irrespective of pH: acetone, methylene chloride, 4-heptanone, 2-pentanone and 2-butanone. A comparative analysis of unfrozen and frozen-thawed urine (stored at room temperature for 0, 1 and 8 h) showed that samples retained the same number of compounds although variations in the peak areas for some compounds were observed. © 2008 IOP Publishing Ltd.
Journal Article Type | Article |
---|---|
Publication Date | Sep 1, 2008 |
Journal | Journal of Breath Research |
Print ISSN | 1752-7155 |
Electronic ISSN | 1752-7163 |
Publisher | IOP Publishing |
Peer Reviewed | Peer Reviewed |
Volume | 2 |
Issue | 3 |
Pages | 037022 |
DOI | https://doi.org/10.1088/1752-7155/2/3/037022 |
Public URL | https://uwe-repository.worktribe.com/output/1022081 |
Publisher URL | http://dx.doi.org/10.1088/1752-7155/2/3/037022 |
You might also like
Evolving unipolar memristor spiking neural networks
(2015)
Journal Article
Cyclic ortho ester fuel additive
(2014)
Patent
Slime mould logic gates based on frequency changes of electrical potential oscillation
(2014)
Journal Article
Evolving spiking networks with variable resistive memories
(2014)
Journal Article
Emergent spiking in non-ideal memristor networks
(2014)
Journal Article
Downloadable Citations
About UWE Bristol Research Repository
Administrator e-mail: repository@uwe.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search