The reliability of rabbit monoclonal antibodies in the immunohistochemical assessment of estrogen receptors, progesterone receptors, and HER2 in human breast carcinomas

Cribb, Edward C.; Dean, Sarah J.R.; Assam, Emma E.; Rhodes, Anthony; Sarson, Julia; Parker, Andrew

Abstract

The reliability of the rabbit monoclonal antibodies SP1, SP2, SP3, and 4B5 was immunohistochemically assessed on a range of 96 invasive breast carcinomas and the results compared with those achieved with established antibody markers for estrogen receptors (6F11), progesterone receptors (PgR636), and HER2 (polyclonal A0485 and clone CB11), with HER2 status validated by fluorescence in situ hybridization (FISH) and silver in situ hybridization. Optimal results depended on the duration of microwave antigen-retrieval time and the use of a high pH buffer for rabbit and mouse estrogen receptor antibodies (SP1 and 6F11), although only on antigen-retrieval duration for the progesterone receptors SP2 and PgR636. The highest rate of concordance between HER2 overexpression and HER2 gene amplification was with the rabbit monoclonal antibodies (SP3 and 4B5) and FISH. Rabbit monoclonal antibodies are reliable alternatives to established antibody markers for the immunohistochemical testing of estrogen receptors, progesterone receptors, and HER2 in breast cancer. © American Society for Clinical Pathology.

Citation

Cribb, E. C., Dean, S. J., Assam, E. E., Rhodes, A., Sarson, J., & Parker, A. (2010). The reliability of rabbit monoclonal antibodies in the immunohistochemical assessment of estrogen receptors, progesterone receptors, and HER2 in human breast carcinomas. American Journal of Clinical Pathology, 134(4), 621-632. https://doi.org/10.1309/AJCPOG3O3KTPZQNK

• Journal Article Type: Article
• Publication Date: Jan 1, 2010
• Deposit Date: Aug 27, 2010
• Publicly Available Date: Mar 3, 2016
• Journal: American Journal of Clinical Pathology
• Print ISSN: 1943-7722
• Electronic ISSN: 1943-7722
• Publisher: Oxford University Press (OUP)
• Peer Reviewed: Peer Reviewed
• Volume: 134
• Issue: 4
• Pages: 621-632
• DOI: https://doi.org/10.1309/AJCPOG3O3KTPZQNK
• Keywords: rabbit monoclonal antibodies, immunohistochemistry, fluorescence in situ hybridization, FISH, silver in situ hybridization, SISH, estrogen receptors, progesterone receptors, HER2
• Public URL: https://uwe-repository.worktribe.com/output/974738
• Publisher URL: http://dx.doi.org/10.1309/AJCPOG3O3KTPZQNK