Dendritic cell migration assay: A potential prediction model for identification of contact allergens

McLeod, Julie; Gibbs, Susan; Spiekstra, Sander; Corsini, Emanuela; Reinders, Judith

Abstract

This manuscript describes methodology and a prediction model for the MUTZ-LC migration assay. The assay represents the physiological change in Langerhans cell (LC) behavior after exposure to a sensitizing chemical, resulting in LC migration from the epidermis to the dermis. MUTZ-LC are derived from the commercially available MUTZ-3 cell line. Upon exposure to a sensitizer MUTZ-LC migrate preferentially towards CXCL12 whereas upon exposure to a non-sensitizer MUTZ-LC migrate towards CCL5. A CXCL12/CCL5 ratio >1.10 in 2/3 independent experiments is indicative of a sensitizer, whereas a CXCL12/CCL5 ratio 80%); (iii) standard curve to optimize correlation of fluorescence with cell number; and (iv) optimization of the concentration of rhCXCL12 and rhCCL5 in transwell. The protocol has been tested in three European laboratories and results suggest that it may provide working conditions for performing the DC migration assay which is aimed at distinguishing sensitizers from non sensitizers. © 2012 Elsevier Ltd.

Citation

McLeod, J., Gibbs, S., Spiekstra, S., Corsini, E., & Reinders, J. (2013). Dendritic cell migration assay: A potential prediction model for identification of contact allergens. Toxicology in Vitro, 27(3), 1170-1179. https://doi.org/10.1016/j.tiv.2012.05.016

• Journal Article Type: Article
• Publication Date: Apr 1, 2013
• Deposit Date: Jan 23, 2013
• Journal: Toxicology in Vitro
• Print ISSN: 0887-2333
• Electronic ISSN: 1879-3177
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 27
• Issue: 3
• Pages: 1170-1179
• DOI: https://doi.org/10.1016/j.tiv.2012.05.016
• Keywords: biosensors, skin sensitisers, immune, MUTZ-3, migration, ; sensitizer, dendritic cell, in vitro toxicology, alternative method, chemokine
• Public URL: https://uwe-repository.worktribe.com/output/946465
• Publisher URL: http://dx.doi.org/10.1016/j.tiv.2012.05.016