Anita Mitra
Comparison of vaginal microbiota sampling techniques: Cytobrush versus swab
Mitra, Anita; MacIntyre, David A.; Mahajan, Vishakha; Lee, Yun S.; Smith, Ann; Marchesi, Julian R.; Lyons, Deirdre; Bennett, Phillip R.; Kyrgiou, Maria
Authors
David A. MacIntyre
Vishakha Mahajan
Yun S. Lee
Ann Smith
Julian R. Marchesi
Deirdre Lyons
Phillip R. Bennett
Maria Kyrgiou
Abstract
© 2017 The Author(s). Evidence suggests the vaginal microbiota (VM) may influence risk of persistent Human Papillomavirus (HPV) infection and cervical carcinogenesis. Established cytology biobanks, typically collected with a cytobrush, constitute a unique resource to study such associations longitudinally. It is plausible that compared to rayon swabs; the most commonly used sampling devices, cytobrushes may disrupt biofilms leading to variation in VM composition. Cervico-vaginal samples were collected with cytobrush and rayon swabs from 30 women with high-grade cervical precancer. Quantitative PCR was used to compare bacterial load and Illumina MiSeq sequencing of the V1-V3 regions of the 16S rRNA gene used to compare VM composition. Cytobrushes collected a higher total bacterial load. Relative abundance of bacterial species was highly comparable between sampling devices (R 2 = 0.993). However, in women with a Lactobacillus-depleted, high-diversity VM, significantly less correlation in relative species abundance was observed between devices when compared to those with a Lactobacillus species-dominant VM (p = 0.0049). Cytobrush and swab sampling provide a comparable VM composition. In a small proportion of cases the cytobrush was able to detect underlying high-diversity community structure, not realized with swab sampling. This study highlights the need to consider sampling devices as potential confounders when comparing multiple studies and datasets.
Journal Article Type | Article |
---|---|
Acceptance Date | Jul 31, 2017 |
Online Publication Date | Aug 29, 2017 |
Publication Date | Dec 1, 2017 |
Deposit Date | Oct 2, 2019 |
Publicly Available Date | Oct 4, 2019 |
Journal | Scientific Reports |
Electronic ISSN | 2045-2322 |
Publisher | Nature Research (part of Springer Nature) |
Peer Reviewed | Peer Reviewed |
Volume | 7 |
Issue | 1 |
DOI | https://doi.org/10.1038/s41598-017-09844-4 |
Public URL | https://uwe-repository.worktribe.com/output/3453296 |
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Comparison of vaginal microbiota sampling techniques: cytobrush versus swab
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