Fungal elicitor Pep-25 increases cytosolic calcium ions, H 2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana

Hu, Xiangyang; Neill, Steven; Yang, Yongping; Cai, Weiming

Abstract

Transgenic Arabidopsis thaliana plants expressing the protein aequorin were used to investigate the transient change in cytosolic calcium ions caused by stimulation through the fungal elicitor Pep-25. Our results show that the elicitor Pep-25 derived from Phytophthora sojae can induce H2O 2 production and an increase in cytosolic calcium ions. The transcription of LOX, OPR3, PED, AOS, AOC genes and the protein accumulation of AOS were induced by Pep-25 treatment. Pep-25 also induced an accumulation of jasmonic acid (JA). Blocking the production of H2O2 and the increase of cytosolic calcium ions both suppressed the transcription of LOX, OPR3, PED, AOS, AOC genes, the accumulation of AOS, and the accumulation of JA. These results indicated that the production of H2O2 derived from the plasma-membrane NADPH oxidase and the subsequently increase of cytosolic calcium ions are both required for the activation of the octadecanoid pathway by Pep-25 treatment in A. thaliana. © 2009 Springer-Verlag.

• Journal Article Type: Article
• Publication Date: May 1, 2009
• Journal: Planta
• Print ISSN: 0032-0935
• Electronic ISSN: 1432-2048
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 229
• Issue: 6
• Pages: 1201-1208
• DOI: https://doi.org/10.1007/s00425-009-0909-3
• Keywords: Arabidopsis, aequorin, cytosolic calcium ions, hydrogen peroxide, octadecanoid pathway
• Public URL: https://uwe-repository.worktribe.com/output/1003235
• Publisher URL: http://dx.doi.org/10.1007/s00425-009-0909-3