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Upregulation of oxidative stress markers in human microvascular endothelial cells by complexes of serum albumin and digestion products of glycated casein

Glenn, Josephine V.; Powell, Lesley A.; Stitt, Alan W.; Powell, Lesley; Deo, Permal; Ames, Jennifer

Authors

Josephine V. Glenn

Lesley A. Powell

Alan W. Stitt

Lesley Powell

Permal Deo

Jennifer Ames



Abstract

The extent of absorption of dietary advanced glycation end products (AGEs) is not fully known. The possible physiological impact of these absorbed components on inflammatory processes has been studied little and was the aim of this investigation. Aqueous solutions of bovine casein and glucose were heated at 95°C for 5 h to give AGE-casein (AGE-Cas). Simulated stomach and small intestine digestion of AGE-Cas and dialysis (molecular mass cutoff of membrane = 1 kDa) resulted in a low molecular mass (LMM) fraction of digestion products, which was used to prepare bovine serum albumin (BSA)-LMM-AGE-Cas complexes. Stimulation of human microvascular endothelial cells with BSA-LMM-AGE-Cas complexes significantly increased mRNA expression of the receptor of AGE (RAGE), galectin-3 (AGE-R3), tumor necrosis factor alpha, and a marker of the mitogen-activated protein kinase pathway (MAPK-1), as well as p65NF-κB activation. Cells treated with LMM digestion products of AGE-Cas significantly increased AGE-R3 mRNA expression. Intracellular reactive oxygen species production increased significantly in cells challenged with BSA-LMM-AGE-Cas and LMM-AGE-Cas. In conclusion, in an in vitro cell system, digested dietary AGEs complexed with serum albumin play a role in the regulation of RAGE and downstream inflammatory pathways. AGE-R3 may protect against these effects. © 2009 Wiley Periodicals, Inc.

Citation

Ames, J. M., Stitt, A. W., Powell, L. A., Glenn, J. V., Deo, P., Glenn, J., …Ames, J. (2009). Upregulation of oxidative stress markers in human microvascular endothelial cells by complexes of serum albumin and digestion products of glycated casein. Journal of Biochemical and Molecular Toxicology, 23(5), 364-372. https://doi.org/10.1002/jbt.20301

Journal Article Type Article
Publication Date Sep 1, 2009
Journal Journal of Biochemical and Molecular Toxicology
Print ISSN 1095-6670
Electronic ISSN 1099-0461
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 23
Issue 5
Pages 364-372
DOI https://doi.org/10.1002/jbt.20301
Keywords advanced glycation end products, receptor for advanced glycation end products, nuclear factor-κB, tumor necrosis factor alpha, reactive oxygen species
Public URL https://uwe-repository.worktribe.com/output/1001897
Publisher URL http://dx.doi.org/10.1002/jbt.20301