Outputs (48)

Study of overload for basic compounds in reversed-phase high performance liquid chromatography as a function of mobile phase pH (2006)
Journal Article

The retention and overloading properties for eight basic solutes and two quaternary ammonium compounds were studied over the pH range 2.7-10.0 using phosphate and carbonate buffers. At low pH, a hybrid inorganic-organic silica-ODS phase (XTerra RP-18... Read More about Study of overload for basic compounds in reversed-phase high performance liquid chromatography as a function of mobile phase pH.

Overload for ionized solutes in reversed-phase high-performance liquid chromatography (2006)
Journal Article

Overloading occurs for submicrogram quantities of ionized solutes particularly when using low ionic strength mobile phases at low pH (e.g., formic acid), even with highly inert silica RP-HPLC columns of normal dimensions. Much higher loads can produc... Read More about Overload for ionized solutes in reversed-phase high-performance liquid chromatography.

Study of overloading of basic drugs and peptides in reversed-phase high-performance liquid chromatography using pH adjustment of weak acid mobile phases suitable for mass spectrometry (2005)
Journal Article

Serious losses in column efficiency for ionised basic drugs and peptides occur due to overloading of C18 phases when weak acid mobile phases of low ionic strength suitable for mass spectrometric detection are used. Measurable changes in retention tim... Read More about Study of overloading of basic drugs and peptides in reversed-phase high-performance liquid chromatography using pH adjustment of weak acid mobile phases suitable for mass spectrometry.

Effect of buffer on peak shape of peptides in reversed-phase high performance liquid chromatography (2004)
Journal Article

The effect of changing the buffer at constant low pH in the mobile phase is investigated with respect to the separation of a mixture of basic peptides. Considerably worse peak shapes, leading to poorer resolution of complex peptide mixtures, were obt... Read More about Effect of buffer on peak shape of peptides in reversed-phase high performance liquid chromatography.

Rationalization of retention and overloading behavior of basic compounds in reversed-phase HPLC using low ionic strength buffers suitable for mass spectrometric detection (2003)
Journal Article

The retention and overloading behavior of some basic (and acidic) compounds has been studied on different RP-HPLC columns in buffers of varying ionic strength. Anomalous retention patterns of acids and bases were found on one phase in low-pH, volatil... Read More about Rationalization of retention and overloading behavior of basic compounds in reversed-phase HPLC using low ionic strength buffers suitable for mass spectrometric detection.

Overloading study of bases using polymeric RP-HPLC columns as an aid to rationalization of overloading on silica-ODS phases (2002)
Journal Article

The separation of ionized bases by reversed-phase liquid chromatography with alkyl silica columns often leads to severely tailed bands that are highly detrimental. Band shape and its dependence on sample mass are notably different when mobile-phase p... Read More about Overloading study of bases using polymeric RP-HPLC columns as an aid to rationalization of overloading on silica-ODS phases.

Bile metabolites of polycyclic aromatic hydrocarbons in three species of fish from the severn estuary (2002)
Journal Article

Six metabolites of polycyclic aromatic hydrocarbons (PAHs) were identified and quantified from the bile of 31 common eels (Anguilla anguilla), 29 European flounders (Pleuronectes flesus), and 15 conger eels (Conger conger) collected from the Severn E... Read More about Bile metabolites of polycyclic aromatic hydrocarbons in three species of fish from the severn estuary.

Analysis of estrogens in river waters and effluents (2001)
Journal Article