All Outputs (3)

A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM) (2007)
Journal Article
Allen, T. D., Rutherford, S. A., Murray, S., Sanderson, H., Gardiner, F., Kiseleva, E., …Drummond, S. P. (2007). A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM). Nature Protocols, 2(5), 1166-1172. https://doi.org/10.1038/nprot.2007.137

This protocol details methods for the isolation of oocyte nuclear envelopes (NEs) from the African clawed toad Xenopus laevis, immunogold labeling of component proteins and subsequent visualization by field-emission scanning electron microscopy (FESE... Read More about A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM).

Cell biology: Ran, mitosis and the cancer connection (2006)
Journal Article
Sanderson, H. S., & Clarke, P. R. (2006). Cell biology: Ran, mitosis and the cancer connection. Current Biology, 16(12), PR466-R468. https://doi.org/10.1016/j.cub.2006.05.032

The small GTPase Ran has been shown to regulate HURP, a protein that interacts with several mitotic spindle assembly factors. This discovery sheds new light on the role of Ran in the fidelity of mitosis and in cancer. © 2006 Elsevier Ltd. All rights... Read More about Cell biology: Ran, mitosis and the cancer connection.

High resolution analysis of mammalian nuclear structure throughout the cell cycle: Implications for nuclear pore complex assembly during interphase and mitosis (2006)
Journal Article
Drummond, S. P., Rutherford, S. A., Sanderson, H. S., & Allen, T. D. (2006). High resolution analysis of mammalian nuclear structure throughout the cell cycle: Implications for nuclear pore complex assembly during interphase and mitosis. Canadian Journal of Physiology and Pharmacology, 84(3-4), 423-430. https://doi.org/10.1139/y05-148

Nuclear pore complexes (NPCs) are the gateways for both active and passive bidirectional molecular transport between the nucleoplasm and cytoplasm. These mega-dalton assemblies are composed of multiple copies of approximately 30 distinct proteins ter... Read More about High resolution analysis of mammalian nuclear structure throughout the cell cycle: Implications for nuclear pore complex assembly during interphase and mitosis.