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Adrenomedullin receptors on human T cells are glucocorticoid-sensitive

Liverani, Elisabetta; McLeod, Julie D.; Paul, Carolyn

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Authors

Elisabetta Liverani

Julie D. McLeod



Abstract

Adrenomedullin (AM) is a novel vasodilatatory peptide which acts primarily through the calcitonin receptor-like receptor (CLR) in combination with either receptor-activity-modifying-protein (RAMP) 2 or 3 (forming receptors, AM 1 and AM2 respectively). AM plays an important role during inflammation, with its expression increasing following cytokine treatment, promoting macrophage action in situ and high expression by T cells during hypoxic conditions. Examination of T cell AM receptor expression has previously been incomplete, hence we here consider the presentation of AM receptors and their responsiveness to AM and glucocorticoids (GC). AM receptor expression was examined by PCR and flow cytometry in primary human T cells, revealing that RAMP2, 3 and CLR are physiologically expressed in unstimulated T cells, both intracellularly and on the cell surface. PHA stimulation decreased receptor proteins, significantly so for CLR and RAMP3. Incubation with AM elicited limited receptor alterations however, GC treatment (10- 6 M; 24 h) markedly affected cell surface expression, significantly increasing receptor components in unstimulated cells and significantly decreasing the same in stimulated T cells. Our findings indicate that human T cells utilize both AM1 and AM2 receptors, which are GC-sensitive in an activation-state dependent manner. © 2012 Elsevier B.V.

Citation

Liverani, E., McLeod, J. D., & Paul, C. (2012). Adrenomedullin receptors on human T cells are glucocorticoid-sensitive. International Immunopharmacology, 14(1), 75-81. https://doi.org/10.1016/j.intimp.2012.06.011

Journal Article Type Article
Publication Date Sep 1, 2012
Deposit Date Jan 23, 2013
Publicly Available Date Feb 14, 2016
Journal International Immunopharmacology
Print ISSN 1567-5769
Electronic ISSN 1878-1705
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 14
Issue 1
Pages 75-81
DOI https://doi.org/10.1016/j.intimp.2012.06.011
Keywords brain, blood brain barrier, immune, multiple sclerosis
Public URL https://uwe-repository.worktribe.com/output/946111
Publisher URL http://dx.doi.org/10.1016/j.intimp.2012.06.011

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