WT1 interacts with the splicing protein RBM4 and regulates its ability to modulate alternative splicing in vivo

Markus, M. Andrea; Heinrich, Bettina; Raitskin, Oleg; Adams, David J.; Mangs, Helena; Goy, Christine; Ladomery, Michael; Sperling, Ruth; Stamm, Stefan; Morris, Brian J.

Abstract

Wilm's tumor protein 1 (WT1), a protein implicated in various cancers and developmental disorders, consists of two major isoforms: WT1(-KTS), a transcription factor, and WT1(+KTS), a post-transcriptional regulator that binds to RNA and can interact with splicing components. Here we show that WT1 interacts with the novel splicing regulator RBM4. Each protein was found to colocalize in nuclear speckles and to cosediment with supraspliceosomes in glycerol gradients. RBM4 conferred dose-dependent and cell-specific regulation of alternative splicing of pre-mRNAs transcribed from several reporter genes. We found that overexpressed WT1(+KTS) abrogated this effect of RBM4 on splice-site selection, whereas WT1(-KTS) did not. We conclude that the (+KTS) form of WT1 is able to inhibit the effect of RBM4 on alternative splicing. © 2006 Elsevier Inc. All rights reserved.

• Journal Article Type: Article
• Publication Date: Oct 15, 2006
• Journal: Experimental Cell Research
• Print ISSN: 0014-4827
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 312
• Issue: 17
• Pages: 3379-3388
• DOI: https://doi.org/10.1016/j.yexcr.2006.07.008
• Keywords: alternative splicing, RNA binding proteins, speckles, Wilm's tumor suppressor, supraspliceosome
• Public URL: https://uwe-repository.worktribe.com/output/1036054
• Publisher URL: http://dx.doi.org/10.1016/j.yexcr.2006.07.008