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Outputs (66)

Going circular: history, present, and future of circRNAs in cancer. (2023)
Journal Article
Pisignano, G., Michael, D. C., Pirlog, R., Visal, T. H., Ladomery, M., & Calin, G. A. (2023). Going circular: history, present, and future of circRNAs in cancer. Oncogene, 42, 2783–2800. https://doi.org/10.1038/s41388-023-02780-w

To date, thousands of highly abundant and conserved single-stranded RNA molecules shaped into ring structures (circRNAs) have been identified. CircRNAs are multifunctional molecules that have been shown to regulate gene expression transcriptionally a... Read More about Going circular: history, present, and future of circRNAs in cancer..

SPHINX-based combination therapy as a potential novel treatment strategy for acute myeloid leukaemia (2023)
Journal Article
Wodi, C., Belali, T., Morse, R., Porazinski, S., & Ladomery, M. (2023). SPHINX-based combination therapy as a potential novel treatment strategy for acute myeloid leukaemia. British Journal of Biomedical Science, 80, 11041. https://doi.org/10.3389/bjbs.2023.11041

Introduction: Dysregulated alternative splicing is a prominent feature of cancer. The inhibition and knockdown of the SR splice factor kinase SRPK1 reduces tumour growth in vivo. As a result several SPRK1 inhibitors are in development including SPHIN... Read More about SPHINX-based combination therapy as a potential novel treatment strategy for acute myeloid leukaemia.

CDC2-like (CLK) protein kinase inhibition as a novel targeted therapeutic strategy in prostate cancer (2021)
Journal Article
Uzor, S., Porazinski, S., Li, L., Clark, B., Ajiro, M., Iida, K., …Ladomery, M. (2021). CDC2-like (CLK) protein kinase inhibition as a novel targeted therapeutic strategy in prostate cancer. Scientific Reports, 11(1), Article 7963. https://doi.org/10.1038/s41598-021-86908-6

Dysregulation of alternative splicing is a feature of cancer, both in aetiology and progression. It occurs because of mutations in splice sites or sites that regulate splicing, or because of the altered expression and activity of splice factors and o... Read More about CDC2-like (CLK) protein kinase inhibition as a novel targeted therapeutic strategy in prostate cancer.

Epigenenetic regulation of alternative splicing: How lncRNAs tailor the message (2021)
Journal Article
Pisignano, G., & Ladomery, M. (2021). Epigenenetic regulation of alternative splicing: How lncRNAs tailor the message. Non-Coding RNA, 7(1), Article 21. https://doi.org/10.3390/ncrna7010021

Alternative splicing is a highly fine-tuned regulated process and one of the main drivers of proteomic diversity across eukaryotes. The vast majority of human multi-exon genes is alternatively spliced in a cell type-and tissue-specific manner, and de... Read More about Epigenenetic regulation of alternative splicing: How lncRNAs tailor the message.

WT1 activates transcription of the splice factor kinase SRPK1 gene in PC3 and K562 cancer cells in the absence of corepressor BASP1 (2020)
Journal Article
Belali, T., Wodi, C., Clark, B., Cheung, M. K., Craig, T. J., Wheway, G., …Ladomery, M. (2020). WT1 activates transcription of the splice factor kinase SRPK1 gene in PC3 and K562 cancer cells in the absence of corepressor BASP1. BBA - Gene Regulatory Mechanisms, 1863(12), Article 194642. https://doi.org/10.1016/j.bbagrm.2020.194642

Dysregulated alternative splicing plays a prominent role in all hallmarks of cancer. The splice factor kinase SRPK1 drives the activity of oncogenic splice factors such as SRSF1. SRSF1 in turn promotes the expression of splice isoforms that favour tu... Read More about WT1 activates transcription of the splice factor kinase SRPK1 gene in PC3 and K562 cancer cells in the absence of corepressor BASP1.

Targeting the ERG oncogene with splice-switching oligonucleotides as a novel therapeutic strategy in prostate cancer (2020)
Journal Article
Li, L., Hobson, L., Perry, L., Clark, B., Heavey, S., Haider, A., …Ladomery, M. (2020). Targeting the ERG oncogene with splice-switching oligonucleotides as a novel therapeutic strategy in prostate cancer. British Journal of Cancer, 123, 1024-1032. https://doi.org/10.1038/s41416-020-0951-2

Background: The ERG oncogene, a member of the ETS family of transcription factor encoding genes, is a genetic driver of prostate cancer. It is activated through a fusion with the androgen-responsive TMPRSS2 promoter in 50% of cases. There is therefor... Read More about Targeting the ERG oncogene with splice-switching oligonucleotides as a novel therapeutic strategy in prostate cancer.

APC and AXIN2 are promising biomarker candidates for the early detection of adenomas and hyperplastic polyps (2020)
Journal Article
Almond, S., Anaraki, F., Young, C., Ashrafian Bonab, M., Asadzadeh-Aghdaei, H., Rezvani, H., …Ladomery, M. (2020). APC and AXIN2 are promising biomarker candidates for the early detection of adenomas and hyperplastic polyps. Cancer Informatics, 19, 1-8. https://doi.org/10.1177/1176935120972383

Aberrant activation of the WNT/CTNNB1 pathway is notorious in colorectal cancer (CRC). Here, we demonstrate that the expression of specific and crucial WNT signaling pathway genes is linked to disease progression in colonic adenomatous (AP) and hyper... Read More about APC and AXIN2 are promising biomarker candidates for the early detection of adenomas and hyperplastic polyps.

Abscisic acid induced a negative geotropic response in dark-incubated Chlamydomonas reinhardtii (2019)
Journal Article
Al-Hijab, L., Gregg, A., Davies, R., Macdonald, H., Ladomery, M., & Wilson, I. (2019). Abscisic acid induced a negative geotropic response in dark-incubated Chlamydomonas reinhardtii. Scientific Reports, 9(1), Article 12063. https://doi.org/10.1038/s41598-019-48632-0

© 2019, The Author(s). The phytohormone abscisic acid (ABA) plays a role in stresses that alter plant water status and may also regulate root gravitropism and hydrotropism. ABA also exists in the aquatic algal progenitors of land plants, but other th... Read More about Abscisic acid induced a negative geotropic response in dark-incubated Chlamydomonas reinhardtii.

Phenethyl isothiocyanate hampers growth and progression of HER2-positive breast and ovarian carcinoma by targeting their stem cell compartment (2019)
Journal Article
Koschorke, A., Faraci, S., Giani, D., Chiodoni, C., Iorio, E., Canese, R., …Pupa, S. M. (2019). Phenethyl isothiocyanate hampers growth and progression of HER2-positive breast and ovarian carcinoma by targeting their stem cell compartment. Cellular Oncology, 42(6), 815-828. https://doi.org/10.1007/s13402-019-00464-w

© 2019, International Society for Cellular Oncology. Purpose: Isothiocyanates elicit anticancer effects by targeting cancer stem cells (CSCs). Here, we tested the antitumor activity of phenethyl-isothiocyanate (PEITC), either alone or in combination... Read More about Phenethyl isothiocyanate hampers growth and progression of HER2-positive breast and ovarian carcinoma by targeting their stem cell compartment.

Investigating ROS, RNS, and H2S-sensitive signaling proteins (2019)
Book Chapter
Williams, E., Whiteman, M., Wood, M. E., Wilson, I. D., Ladomery, M. R., Allainguillaume, J., …Hancock, J. T. (2019). Investigating ROS, RNS, and H2S-sensitive signaling proteins. In J. T. Hancock, & M. E. Conway (Eds.), Redox-Mediated Signal Transduction (27-42). Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9463-2_3

© Springer Science+Business Media, LLC, part of Springer Nature 2019. The modification of proteins is a key way to alter their activity and function. Often thiols, cysteine residues, on proteins are attractive targets for such modification. Assuming... Read More about Investigating ROS, RNS, and H2S-sensitive signaling proteins.

Altered VEGF splicing isoform balance in tumor endothelium involves activation of splicing factors Srpk1 and Srsf1 by the Wilms’ tumor suppressor Wt1 (2019)
Journal Article
Ladomery, M. (2019). Altered VEGF splicing isoform balance in tumor endothelium involves activation of splicing factors Srpk1 and Srsf1 by the Wilms’ tumor suppressor Wt1. Cells, 8(1), 41. https://doi.org/10.339010.3390/cells8010041

Angiogenesis is one hallmark of cancer. Vascular endothelial growth factor (VEGF) is a known inducer of angiogenesis. Many patients benefit from antiangiogenic therapies, which however have limitations. Although VEGF is overexpressed in most tumors,... Read More about Altered VEGF splicing isoform balance in tumor endothelium involves activation of splicing factors Srpk1 and Srsf1 by the Wilms’ tumor suppressor Wt1.

The Evolutionarily Conserved Cassette Exon 7b Drives ERG's Oncogenic Properties (2018)
Journal Article
Jumbe, S., Porazinski, S., Oltean, S., Mansell, J., Vahabi, B., Wilson, I., & Ladomery, M. (2019). The Evolutionarily Conserved Cassette Exon 7b Drives ERG's Oncogenic Properties. Translational Oncology, 12(1), 134-142. https://doi.org/10.1016/j.tranon.2018.09.001

© 2018 The oncogene ERG encodes an ETS family transcription factor and is implicated in blood, vascular, and bone development and in prostate, blood, and bone cancer. The ERG gene is alternatively spliced; of particular interest is its cassette exon... Read More about The Evolutionarily Conserved Cassette Exon 7b Drives ERG's Oncogenic Properties.

Autoregulation of the human splice factor kinase CLK1 through exon skipping and intron retention (2018)
Journal Article
Uzor, S., Zorzou, P., Bowler, E., Porazinski, S., Wilson, I. D., & Ladomery, M. (2018). Autoregulation of the human splice factor kinase CLK1 through exon skipping and intron retention. Gene, 670, 46-54. https://doi.org/10.1016/j.gene.2018.05.095

© 2018 Elsevier B.V. Alternative splicing is a key process required for the regulation of gene expression in normal development and physiology. It is regulated by splice factors whose activities are in turn regulated by splice factor kinases and phos... Read More about Autoregulation of the human splice factor kinase CLK1 through exon skipping and intron retention.

Targeting splicing in prostate cancer (2018)
Journal Article
Antonopoulou, E., & Ladomery, M. (2018). Targeting splicing in prostate cancer. International Journal of Molecular Sciences, 19(5), 1287. https://doi.org/10.3390/ijms19051287

© 2018 by the authors. Licensee MDPI, Basel, Switzerland. Over 95% of human genes are alternatively spliced, expressing splice isoforms that often exhibit antagonistic functions. We describe genes whose alternative splicing has been linked to prostat... Read More about Targeting splicing in prostate cancer.

Hypoxia leads to significant changes in alternative splicing and elevated expression of CLK splice factor kinases in PC3 prostate cancer cells (2018)
Journal Article
Bowler, E., Porazinski, S., Uzor, S., Thibault, P., Durand, M., Lapointe, E., …Ladomery, M. (2018). Hypoxia leads to significant changes in alternative splicing and elevated expression of CLK splice factor kinases in PC3 prostate cancer cells. BMC Cancer, 18(1), 355. https://doi.org/10.1186/s12885-018-4227-7

© 2018 The Author(s). Background: Mounting evidence suggests that one of the ways that cells adapt to hypoxia is through alternative splicing. The aim of this study was firstly to examine the effect of hypoxia on the alternative splicing of cancer as... Read More about Hypoxia leads to significant changes in alternative splicing and elevated expression of CLK splice factor kinases in PC3 prostate cancer cells.

Alternative splicing in the hippo pathway-implications for disease and potential therapeutic targets (2018)
Journal Article
Porazinski, S., & Ladomery, M. (2018). Alternative splicing in the hippo pathway-implications for disease and potential therapeutic targets. Genes, 9(3), 161. https://doi.org/10.3390/genes9030161

© 2018 by the authors. Licensee MDPI, Basel, Switzerland. Alternative splicing is a well-studied gene regulatory mechanism that produces biological diversity by allowing the production of multiple protein isoforms from a single gene. An involvement o... Read More about Alternative splicing in the hippo pathway-implications for disease and potential therapeutic targets.

The oncogenic transcription factor ERG represses the transcription of the tumour suppressor gene PTEN in prostate cancer cells (2017)
Journal Article
Adamo, P., Porazinski, S., Rajatileka, S., Jumbe, S., Hagen, R., Cheung, M. K., …Ladomery, M. (2017). The oncogenic transcription factor ERG represses the transcription of the tumour suppressor gene PTEN in prostate cancer cells. Oncology Letters, 14(5), 5605-5610. https://doi.org/10.3892/ol.2017.6841

© 2017, Spandidos Publications. All rights reserved. The oncogene ETS-related gene (ERG) encodes a transcription factor with roles in the regulation of haematopoiesis, angiogenesis, vasculogenesis, inflammation, migration and invasion. The ERG oncoge... Read More about The oncogenic transcription factor ERG represses the transcription of the tumour suppressor gene PTEN in prostate cancer cells.

Residual ground-water levels of the neonicotinoid thiacloprid perturb chemosensing of Caenorhabditis elegans (2017)
Journal Article
Floyd, K. G., Hopewell, H., Burnell, D., Hancock, J. T., Allainguillaume, J., Ladomery, M., & Wilson, I. D. (2017). Residual ground-water levels of the neonicotinoid thiacloprid perturb chemosensing of Caenorhabditis elegans. Ecotoxicology, 26(7), 981-990. https://doi.org/10.1007/s10646-017-1826-z

© 2017, The Author(s). This study investigated the neurological effects of residual ground-water levels of thiaclopridon the non-target organism Caenorhabditis elegans. Nematodes treated with thiacloprid showed a dose-dependent and significantly incr... Read More about Residual ground-water levels of the neonicotinoid thiacloprid perturb chemosensing of Caenorhabditis elegans.

Insulin receptor isoform variations in prostate cancer cells (2016)
Journal Article
Perks, C. M., Zielinska, H. A., Wang, J., Jarrett, C., Frankow, A., Ladomery, M. R., …Holly, J. M. P. (2016). Insulin receptor isoform variations in prostate cancer cells. Frontiers in Endocrinology, 7(SEP), https://doi.org/10.3389/fendo.2016.00132

� 2016 Perks, Zielinska, Wang, Jarrett, Frankow, Ladomery, Bahl, Rhodes, Oxley and Holly. Men who develop prostate cancer (PCa) increasingly have one of the co-morbidities associated with a Western lifestyle that are characterized by hyperinsulinem... Read More about Insulin receptor isoform variations in prostate cancer cells.

Oligonucleotide-based systems: DNA, microRNAs, DNA/RNA aptamers (2016)
Journal Article
Jolly, P., Estrela, P., & Ladomery, M. (2016). Oligonucleotide-based systems: DNA, microRNAs, DNA/RNA aptamers. Essays in Biochemistry, 60(1), 27-35. https://doi.org/10.1042/EBC20150004

© 2016 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society. There are an increasing number of applications that have been developed for oligonucleotide-based biosensing systems in genetics and biomedicine. Oligonuc... Read More about Oligonucleotide-based systems: DNA, microRNAs, DNA/RNA aptamers.

The oncogene ERG: A key factor in prostate cancer (2016)
Journal Article
Ladomery, M. R., Adamo, P., Adamo, P., & Ladomery, M. (2016). The oncogene ERG: A key factor in prostate cancer. Oncogene, 35(4), 403-414. https://doi.org/10.1038/onc.2015.109

© 2016 Macmillan Publishers Limited All rights reserved. ETS-related gene (ERG) is a member of the E-26 transformation-specific (ETS) family of transcription factors with roles in development that include vasculogenesis, angiogenesis, haematopoiesis... Read More about The oncogene ERG: A key factor in prostate cancer.

The Molecular Biology of RNA (2015)
Book
Elliott, D., & Ladomery, M. (2015). The Molecular Biology of RNA. Oxford: Oxford University Press

The Scd6/Lsm14 protein xRAPB has properties different from RAP55 in selecting mRNA for early translation or intracellular distribution in Xenopus oocytes (2015)
Journal Article
Ladomery, M., & Sommerville, J. (2015). The Scd6/Lsm14 protein xRAPB has properties different from RAP55 in selecting mRNA for early translation or intracellular distribution in Xenopus oocytes. BBA - Gene Regulatory Mechanisms, 1849(11), 1363-1373. https://doi.org/10.1016/j.bbagrm.2015.10.002

© 2015 Elsevier B.V. Oocytes accumulate mRNAs in the form of maternal ribonucleoprotein (RNP) particles, the protein components of which determine the location and stability of individual mRNAs prior to translation. Scd6/Lsm14 proteins, typified by R... Read More about The Scd6/Lsm14 protein xRAPB has properties different from RAP55 in selecting mRNA for early translation or intracellular distribution in Xenopus oocytes.

Detection of thiol modifications by hydrogen sulfide (2015)
Book Chapter
Williams, E., Pead, S., Whiteman, M., Wood, M. E., Wilson, I. D., Ladomery, M., …Hancock, J. T. (2015). Detection of thiol modifications by hydrogen sulfide. In E. Cadenas, & L. Packer (Eds.), Hydrogen Sulfide in Redox Biology Part B (233-251). UK: Academic Press

The evolutionarily conserved multifunctional glycine-rich RNA-binding proteins play key roles in development and stress adaptation (2015)
Journal Article
Wilson, I., Ciuzan, O., Hancock, J. T., Pamfil, D., Wilson, I. D., & Ladomery, M. (2015). The evolutionarily conserved multifunctional glycine-rich RNA-binding proteins play key roles in development and stress adaptation. Physiologia Plantarum, 153(1), 1-11. https://doi.org/10.1111/ppl.12286

© 2014 Scandinavian Plant Physiology Society. The class IV glycine-rich RNA-binding proteins are a distinct subgroup within the heterogenous superfamily of glycine-rich proteins (GRPs). They are distinguished by the presence of an RNA-binding domain... Read More about The evolutionarily conserved multifunctional glycine-rich RNA-binding proteins play key roles in development and stress adaptation.

Serine-arginine protein kinase 1 (SRPK1) inhibition as a potential novel targeted therapeutic strategy in prostate cancer (2014)
Journal Article
Mavrou, A., Brakspear, K., Hamdollah-Zadeh, M., Damodaran, G., Babaei-Jadidi, R., Oxley, J., …Oltean, S. (2015). Serine-arginine protein kinase 1 (SRPK1) inhibition as a potential novel targeted therapeutic strategy in prostate cancer. Oncogene, 34, 4311-4319. https://doi.org/10.1038/onc.2014.360

© 2015 Macmillan Publishers Limited. All rights reserved. Angiogenesis is required for tumour growth and is induced principally by vascular endothelial growth factor A (VEGF-A). VEGF-A pre-mRNA is alternatively spliced at the terminal exon to produce... Read More about Serine-arginine protein kinase 1 (SRPK1) inhibition as a potential novel targeted therapeutic strategy in prostate cancer.

Alternative splicing of TIA-1 in human colon cancer regulates VEGF isoform expression, angiogenesis, tumour growth and bevacizumab resistance (2014)
Journal Article
Hamdollah Zadeh, M. A., Amin, E. M., Hoareau-Aveilla, C., Domingo, E., Symonds, K. E., Ye, X., …Bates, D. O. (2015). Alternative splicing of TIA-1 in human colon cancer regulates VEGF isoform expression, angiogenesis, tumour growth and bevacizumab resistance. Molecular Oncology, 9(1), 167-178. https://doi.org/10.1016/j.molonc.2014.07.017

© 2014 The Authors. The angiogenic capability of colorectal carcinomas (CRC), and their susceptibility to anti-angiogenic therapy, is determined by expression of vascular endothelial growth factor (VEGF) isoforms. The intracellular protein T-cell In... Read More about Alternative splicing of TIA-1 in human colon cancer regulates VEGF isoform expression, angiogenesis, tumour growth and bevacizumab resistance.

Quantitative analysis of ERG expression and its splice isoforms in formalin-fixed, paraffin-embedded prostate cancer samples: Association with seminal vesicle invasion and biochemical recurrence (2014)
Journal Article
Hagen, R. M., Adamo, P., Karamat, S., Oxley, J., Aning, J. J., Gillatt, D., …Rhodes, A. (2014). Quantitative analysis of ERG expression and its splice isoforms in formalin-fixed, paraffin-embedded prostate cancer samples: Association with seminal vesicle invasion and biochemical recurrence. American Journal of Clinical Pathology, 142(4), 533-540. https://doi.org/10.1309/AJCPH88QHXARISUP

© American Society for Clinical Pathology. Objectives: The proto-oncogene ETS-related gene (ERG) is consistently overexpressed in prostate cancer. Alternatively spliced isoforms of ERG have variable biological activities; inclusion of exon 11 (72 bas... Read More about Quantitative analysis of ERG expression and its splice isoforms in formalin-fixed, paraffin-embedded prostate cancer samples: Association with seminal vesicle invasion and biochemical recurrence.

Epigallocatechin-3-gallate promotes apoptosis and expression of the caspase 9a splice variant in PC3 prostate cancer cells (2013)
Journal Article
Ladomery, M. R., Morse, H. R., Bowler, E., Mintoff, C. P., Chedea, V. S., Hagen, R. M., & Ladomery, M. (2013). Epigallocatechin-3-gallate promotes apoptosis and expression of the caspase 9a splice variant in PC3 prostate cancer cells. International Journal of Oncology, 43(1), 194-200. https://doi.org/10.3892/ijo.2013.1920

Growing evidence suggests that the flavonoid epigallocatechin-3-gallate (EGCG), notably abundant in green tea, has health-promoting properties. We examined the effect of EGCG on cell survival and apoptosis in the prostate cancer cell line PC3. Cell s... Read More about Epigallocatechin-3-gallate promotes apoptosis and expression of the caspase 9a splice variant in PC3 prostate cancer cells.

Conjugated linoleate reduces prostate cancer viability whereas the effects of oleate and stearate are cell line-dependent (2013)
Journal Article
Hagen, R., Rhodes, A., & Ladomery, M. (2013). Conjugated linoleate reduces prostate cancer viability whereas the effects of oleate and stearate are cell line-dependent. Anticancer Research, 33(10), 4395-4400

BACKGROUND: In this study, responses to fatty acid treatments in commonly used prostate cancer cell culture models and variability of gene expression between them were determined. MATERIALS AND METHODS: PC3, DU145, LNCaP, VCaP and PNT2 cells... Read More about Conjugated linoleate reduces prostate cancer viability whereas the effects of oleate and stearate are cell line-dependent.

Role of splice variants in the metastatic progression of prostate cancer (2012)
Journal Article
Hagen, R. M., & Ladomery, M. (2012). Role of splice variants in the metastatic progression of prostate cancer. Biochemical Society Transactions, 40(4), 870-874. https://doi.org/10.1042/BST20120026

AS (alternative splicing) and its role in disease, especially cancer, has come to forefront in research over the last few years. Alterations in the ratio of splice variants have been widely observed in cancer. Splice variants of cancer-associated gen... Read More about Role of splice variants in the metastatic progression of prostate cancer.

WT1 Mutants Reveal SRPK1 to Be a Downstream Angiogenesis Target by Altering VEGF Splicing (2011)
Journal Article
Bates, D. O., Hinton, D. R., Harper, S. J., Schumacher, V. A., Saleem, M. A., Nowak, D. G., …Ladomery, M. (2011). WT1 Mutants Reveal SRPK1 to Be a Downstream Angiogenesis Target by Altering VEGF Splicing. Cancer Cell, 20(6), 768-780. https://doi.org/10.1016/j.ccr.2011.10.016

Angiogenesis is regulated by the balance of proangiogenic VEGF 165 and antiangiogenic VEGF 165b splice isoforms. Mutations in WT1, the Wilms' tumor suppressor gene, suppress VEGF 165b and cause abnormal gonadogenesis, renal failure, and Wilms' tumors... Read More about WT1 Mutants Reveal SRPK1 to Be a Downstream Angiogenesis Target by Altering VEGF Splicing.

Kinetic behaviour of WT 1's zinc finger domain in binding to the alpha-actinin-1 mRNA (2010)
Journal Article
Nurmemmedov, E., Yengo, R. K., Ladomery, M., & Thunnissen, M. M. (2010). Kinetic behaviour of WT 1's zinc finger domain in binding to the alpha-actinin-1 mRNA. Archives of Biochemistry and Biophysics, 497(1-2), 21-27. https://doi.org/10.1016/j.abb.2010.02.013

The zinc finger transcription factor Wilms tumour protein (WT 1) is known for its essential involvement in the development of the genitourinary system as well as of other organs and tissues. WT 1 is capable of selectively binding either DNA or mRNA t... Read More about Kinetic behaviour of WT 1's zinc finger domain in binding to the alpha-actinin-1 mRNA.

Regulation of Vascular Endothelial Growth Factor (VEGF) splicing from pro-angiogenic to anti-angiogenic isoforms: A novel therapeutic strategy for angiogenesis (2010)
Journal Article
Amin, E. M., Nowak, D. G., Rennel, E. S., Hoareau-Aveilla, C., Gammons, M., Damodoran, G., …Bates, D. O. (2010). Regulation of Vascular Endothelial Growth Factor (VEGF) splicing from pro-angiogenic to anti-angiogenic isoforms: A novel therapeutic strategy for angiogenesis. Journal of Biological Chemistry, 285(8), 5532-5540. https://doi.org/10.1074/jbc.M109.074930

Vascular endothelial growth factor (VEGF) is produced either as a pro-angiogenic or anti-angiogenic protein depending upon splice site choice in the terminal, eighth exon. Proximal splice site selection (PSS) in exon 8 generates pro-angiogenic isofor... Read More about Regulation of Vascular Endothelial Growth Factor (VEGF) splicing from pro-angiogenic to anti-angiogenic isoforms: A novel therapeutic strategy for angiogenesis.

A proteomic analysis of oligo(dT)-bound mRNP containing oxidative stress-induced Arabidopsis thaliana RNA-binding proteins ATGRP7 and ATGRP8 (2010)
Journal Article
Schmidt, F., Marnef, A., Cheung, M. K., Wilson, I. D., Hancock, J. T., Staiger, D., & Ladomery, M. (2010). A proteomic analysis of oligo(dT)-bound mRNP containing oxidative stress-induced Arabidopsis thaliana RNA-binding proteins ATGRP7 and ATGRP8. Molecular Biology Reports, 37(2), 839-845. https://doi.org/10.1007/s11033-009-9636-x

Plants are highly adapted to respond to a range of environmental stresses commonly by altering their gene expression and metabolism as a result of cell signalling which may be mediated by reactive oxygen species. The glycine-rich RNA-binding proteins... Read More about A proteomic analysis of oligo(dT)-bound mRNP containing oxidative stress-induced Arabidopsis thaliana RNA-binding proteins ATGRP7 and ATGRP8.

P19 H-ras induces G1/S phase delay maintaining cells in a reversible quiescence state (2009)
Journal Article
Camats, M., Kokolo, M., Heesom, K. J., Ladomery, M., Bach-Elias, M., & Blagosklonny, M. V. (2009). P19 H-ras induces G1/S phase delay maintaining cells in a reversible quiescence state. PLoS ONE, 4(12), e8513. https://doi.org/10.1371/journal.pone.0008513

Background: Three functional c-ras genes, known as c-H-ras, c-K-ras, and c-N-ras, have been largely studied in mammalian cells with important insights into normal and tumorigenic cellular signal transduction events. Two K-Ras mRNAs are obtained from... Read More about P19 H-ras induces G1/S phase delay maintaining cells in a reversible quiescence state.

RAP55: Insights into an evolutionarily conserved protein family (2009)
Journal Article
Marnef, A., Sommerville, J., & Ladomery, M. (2009). RAP55: Insights into an evolutionarily conserved protein family. International Journal of Biochemistry and Cell Biology, 41(5), 977-981. https://doi.org/10.1016/j.biocel.2008.06.015

The RAP55 protein family is evolutionarily conserved in eukaryotes. Two highly conserved paralogues, RAP55A and RAP55B, exist in vertebrates; their functional properties and expression patterns remain to be compared. RAP55 proteins share multiple dom... Read More about RAP55: Insights into an evolutionarily conserved protein family.

Expression of pro- and anti-angiogenic isoforms of VEGF is differentially regulated by splicing and growth factors (2008)
Journal Article
Amin, E. M., Nowak, D. G., Woolard, J., Konopatskaya, O., Saleem, M. A., Churchill, A. J., …Bates, D. O. (2008). Expression of pro- and anti-angiogenic isoforms of VEGF is differentially regulated by splicing and growth factors. Journal of Cell Science, 121(20), 3487-3495. https://doi.org/10.1242/jcs.016410

Vascular endothelial growth factor A (VEGFA; hereafter referred to as VEGF) is a key regulator of physiological and pathological angiogenesis. Two families of VEGF isoforms are generated by alternate splice-site selection in the terminal exon. Proxim... Read More about Expression of pro- and anti-angiogenic isoforms of VEGF is differentially regulated by splicing and growth factors.

New insights into the function of the Wilms tumor suppressor gene WT1 in podocytes (2008)
Journal Article
Morrison, A. A., Viney, R. L., Saleem, M. A., & Ladomery, M. (2008). New insights into the function of the Wilms tumor suppressor gene WT1 in podocytes. AJP - Renal Physiology, 295(1), F12-F17. https://doi.org/10.1152/ajprenal.00597.2007

The Wilms tumor suppressor gene WT1 is essential for early urogenital development: homozygous mutations in WT1 result in embryonic lethality due to a failure in the development of kidneys and gonads. In the adult kidney, WT1 expression is limited to... Read More about New insights into the function of the Wilms tumor suppressor gene WT1 in podocytes.

The post-transcriptional roles of WT1, a multifunctional zinc-finger protein (2008)
Journal Article
Morrison, A. A., Viney, R. L., & Ladomery, M. (2008). The post-transcriptional roles of WT1, a multifunctional zinc-finger protein. Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1785(1), 55-62. https://doi.org/10.1016/j.bbcan.2007.10.002

WT1 was first described in 1990 as a tumour suppressor gene associated with Wilms tumour (nephroblastoma). It encodes a typical transcription factor with four C2-H2 zinc fingers in the C-terminus. However WT1 is surprisingly complex at multiple level... Read More about The post-transcriptional roles of WT1, a multifunctional zinc-finger protein.

Alternative splicing in angiogenesis: The vascular endothelial growth factor paradigm (2007)
Journal Article
Ladomery, M., Harper, S. J., & Bates, D. O. (2007). Alternative splicing in angiogenesis: The vascular endothelial growth factor paradigm. Cancer Letters, 249(2), 133-142. https://doi.org/10.1016/j.canlet.2006.08.015

Alternative splicing, first discovered in the 1970s, has emerged as one of the key generators of proteomic diversity. Not surprisingly, alternative splicing is increasingly linked to the etiology of cancer. This is illustrated by vascular endothelial... Read More about Alternative splicing in angiogenesis: The vascular endothelial growth factor paradigm.

A proteomic investigation of glomerular podocytes from a Denys-Drash Syndrome patient with a mutation in the Wilms tumour suppressor gene WT1 (2007)
Journal Article
Van Den Heuver, L. P., Viney, R. L., Morrison, A. A., Ni, L., Mathieson, P. W., Saleem, M. A., & Ladomery, M. (2007). A proteomic investigation of glomerular podocytes from a Denys-Drash Syndrome patient with a mutation in the Wilms tumour suppressor gene WT1. Proteomics, 7(5), 804-815. https://doi.org/10.1002/pmic.200600666

Glomerular podocytes are essential for blood filtration in the kidney underpinned by their unique cytoskeletal morphology. An increasing number of kidney diseases are being associated with key podocyte abnormalities. The Wilms tumour suppressor gene... Read More about A proteomic investigation of glomerular podocytes from a Denys-Drash Syndrome patient with a mutation in the Wilms tumour suppressor gene WT1.

Presence of WT1 in nuclear messenger RNP particles in the human acute myeloid leukemia cell lines HL60 and K562 (2006)
Journal Article
Morrison, A. A., & Ladomery, M. (2006). Presence of WT1 in nuclear messenger RNP particles in the human acute myeloid leukemia cell lines HL60 and K562. Cancer Letters, 244(1), 136-141. https://doi.org/10.1016/j.canlet.2005.12.018

The WT1 gene is a key player in acute myeloid leukaemia, in which it is frequently over-expressed. WT1 encodes a multifunctional zinc finger protein transcription factor, which also binds mRNA. Thus increasing evidence suggests that WT1 works both at... Read More about Presence of WT1 in nuclear messenger RNP particles in the human acute myeloid leukemia cell lines HL60 and K562.

WT1 interacts with the splicing protein RBM4 and regulates its ability to modulate alternative splicing in vivo (2006)
Journal Article
Markus, M. A., Heinrich, B., Raitskin, O., Adams, D. J., Mangs, H., Goy, C., …Morris, B. J. (2006). WT1 interacts with the splicing protein RBM4 and regulates its ability to modulate alternative splicing in vivo. Experimental Cell Research, 312(17), 3379-3388. https://doi.org/10.1016/j.yexcr.2006.07.008

Wilm's tumor protein 1 (WT1), a protein implicated in various cancers and developmental disorders, consists of two major isoforms: WT1(-KTS), a transcription factor, and WT1(+KTS), a post-transcriptional regulator that binds to RNA and can interact w... Read More about WT1 interacts with the splicing protein RBM4 and regulates its ability to modulate alternative splicing in vivo.

The Wilms tumour suppressor protein WT1 (+KTS isoform) binds alpha-actinin 1 mRNA via its zinc-finger domain (2006)
Journal Article
Morrison, A. A., Venables, J. P., Dellaire, G., & Ladomery, M. (2006). The Wilms tumour suppressor protein WT1 (+KTS isoform) binds alpha-actinin 1 mRNA via its zinc-finger domain. Biochemistry and Cell Biology, 84(5), 789-798. https://doi.org/10.1139/O06-065

Mutations in WT1 are associated with developmental syndromes that affect the urogenital system and neoplasms, including Wilms tumour, acute myeloid leukemia, and breast and prostate cancers. The WT1 protein belongs to the early growth response family... Read More about The Wilms tumour suppressor protein WT1 (+KTS isoform) binds alpha-actinin 1 mRNA via its zinc-finger domain.

The Wilms' tumor 1 (WT1) gene (+KTS isoform) functions with a CTE to enhance translation from an unspliced RNA with a retained intron (2006)
Journal Article
Hammarskjöld, M. L., Bor, Y. C., Swartz, J., Morrison, A., Rekosh, D., & Ladomery, M. (2006). The Wilms' tumor 1 (WT1) gene (+KTS isoform) functions with a CTE to enhance translation from an unspliced RNA with a retained intron. Genes and Development, 20(12), 1597-1608. https://doi.org/10.1101/gad.1402306

The Wilms' tumor 1 (WT1) gene plays an important role in mammalian urogenital development, and dysregulation of this gene is observed in many human cancers. Alternative splicing of WT1 RNA leads to the expression of two major protein isoforms, WT1(+K... Read More about The Wilms' tumor 1 (WT1) gene (+KTS isoform) functions with a CTE to enhance translation from an unspliced RNA with a retained intron.

Development of an siRNA-based method for repressing specific genes in renal organ culture and its use to show that the Wt1 tumour suppressor is required for nephron differentiation (2004)
Journal Article
Davies, J. A., Ladomery, M., Hohenstein, P., Michael, L., Shafe, A., Spraggon, L., & Hastie, N. (2004). Development of an siRNA-based method for repressing specific genes in renal organ culture and its use to show that the Wt1 tumour suppressor is required for nephron differentiation. Human Molecular Genetics, 13(2), 235-246. https://doi.org/10.1093/hmg/ddh015

Wt1 is a tumour suppressor gene, mutation of which is a cause of Wilms' tumour, a childhood renal nephroblastoma. Wt1 is expressed in a rich pattern during renal development suggesting that it acts at three stages: determination of the kidney area, t... Read More about Development of an siRNA-based method for repressing specific genes in renal organ culture and its use to show that the Wt1 tumour suppressor is required for nephron differentiation.

Murine Denys-Drash syndrome: Evidence of podocyte de-differentiation and systemic mediation of glomerulosclerosis (2003)
Journal Article
Hastie, N. D., Fleming, S., Patek, C. E., Miles, C. G., Bellamy, C. O., Ladomery, M., …Hooper, M. L. (2003). Murine Denys-Drash syndrome: Evidence of podocyte de-differentiation and systemic mediation of glomerulosclerosis. Human Molecular Genetics, 12(18), 2379-2394. https://doi.org/10.1093/hmg/ddg240

Denys-Drash syndrome (DDS) is caused by dominant mutations of the Wilms' tumour suppressor gene, WT1, and characterized by a nephropathy involving diffuse mesangial sclerosis, male pseudohermaphroditism and/ or Wilms' tumourigenesis. Previously, we r... Read More about Murine Denys-Drash syndrome: Evidence of podocyte de-differentiation and systemic mediation of glomerulosclerosis.

Expression in Xenopus oocytes shows that WT1 binds transcripts in vivo, with a central role for zinc finger one (2003)
Journal Article
Ladomery, M., Sommerville, J., Woolner, S., Slight, J., & Hastie, N. (2003). Expression in Xenopus oocytes shows that WT1 binds transcripts in vivo, with a central role for zinc finger one. Journal of Cell Science, 116(8), 1539-1549. https://doi.org/10.1242/jcs.00324

The Wilms' tumour suppressor gene WT1 encodes a protein involved in urogenital development and disease. The salient feature of WT1 is the presence of four 'Krüppel'-type C2-H2 zinc fingers in the C-terminus. Uniquely to WTI, an evolutionarily conserv... Read More about Expression in Xenopus oocytes shows that WT1 binds transcripts in vivo, with a central role for zinc finger one.

Multifunctional zinc finger proteins in development and disease (2002)
Journal Article
Ladomery, M., & Dellaire, G. (2002). Multifunctional zinc finger proteins in development and disease. Annals of Human Genetics, 66(5-6), 331-342. https://doi.org/10.1046/j.1469-1809.2002.00121.x

Post-transcriptional processes contribute significantly towards the generation of proteomic diversity. An increasing number of mutations have been described that affect genes encoding components of the post-transcriptional machinery. In particular, m... Read More about Multifunctional zinc finger proteins in development and disease.

4SR, a novel zinc-finger protein with SR-repeats, is expressed during early development of Xenopus (2000)
Journal Article
Ladomery, M., Marshall, R., Arif, L., & Sommerville, J. (2000). 4SR, a novel zinc-finger protein with SR-repeats, is expressed during early development of Xenopus. Gene, 256(1-2), 293-302. https://doi.org/10.1016/S0378-1119%2800%2900375-9

The protein C4SR contains two cysteine 4 (C 4) zinc-finger motifs at its amino terminus, a stretch of acidic residues in the middle and a series of serine-arginine (SR) repeats at its carboxyl terminus. A cDNA clone encoding the zinc-finger domain wa... Read More about 4SR, a novel zinc-finger protein with SR-repeats, is expressed during early development of Xenopus.

Presence of WT1, the Wilm's tumor suppressor gene product, in nuclear poly(A)+ ribonucleoprotein (1999)
Journal Article
Hastie, N. D., Ladomery, M. R., Ladomery, M., Slight, J., Mc Ghee, S., & Hastie, N. (1999). Presence of WT1, the Wilm's tumor suppressor gene product, in nuclear poly(A)+ ribonucleoprotein. Journal of Biological Chemistry, 274(51), 36520-36526. https://doi.org/10.1074/jbc.274.51.36520

The tumor suppressor gene WT1 encodes a zinc finger protein, which consists of four C-terminal C2-H2 zinc fingers of the Kruppel type, and at the N terminus a Q/P-rich trans-regulatory domain, both characteristic of transcription factors. However, re... Read More about Presence of WT1, the Wilm's tumor suppressor gene product, in nuclear poly(A)+ ribonucleoprotein.

Multiple roles for the Wilms' tumor suppressor, WT1 (1999)
Journal Article
Davies, R., Moore, A., Schedl, A., Bratt, E., Miyahawa, K., Ladomery, M., …Hastie, N. (1999). Multiple roles for the Wilms' tumor suppressor, WT1. Cancer Research, 59, 1747s-1750s

Wilms' tumor is a childhood kidney tumor that is a striking example of the way that cancer may arise through development gone awry. A proportion of these tumors develop as a result of the loss of function mutations in the Wilms' tumor suppressor gene... Read More about Multiple roles for the Wilms' tumor suppressor, WT1.

Xenopus HDm, a maternally expressed histone deacetylase, belongs to an ancient family of acetyl-metabolizing enzymes (1997)
Journal Article
Ladomery, M., Lyons, S., & Sommerville, J. (1997). Xenopus HDm, a maternally expressed histone deacetylase, belongs to an ancient family of acetyl-metabolizing enzymes. Gene, 198(1-2), 275-280. https://doi.org/10.1016/S0378-1119%2897%2900325-9

Modification of core histones can alter chromatin structure, facilitating the activation and repression of genes. A key example is the acetylation of N-terminal lysines of the core histones. Recently, the mammalian histone deacetylase HD1 was cloned... Read More about Xenopus HDm, a maternally expressed histone deacetylase, belongs to an ancient family of acetyl-metabolizing enzymes.

Multifunctional proteins suggest connections between transcriptional and post-transcriptional processes (1997)
Journal Article
Ladomery, M. (1997). Multifunctional proteins suggest connections between transcriptional and post-transcriptional processes. BioEssays, 19(10), 903-909. https://doi.org/10.1002/bies.950191010

Recent findings indicate that substantial cross-talk may exist between transcriptional and post-transcriptional processes. Firstly, there are suggestions that specific promoters influence the post-transcriptional fate of transcripts, pointing to comm... Read More about Multifunctional proteins suggest connections between transcriptional and post-transcriptional processes.

Xp54, the Xenopus homologue of human RNA helicase p54, is an integral component of stored mRNP particles in oocytes (1997)
Journal Article
Ladomery, M., Wade, E., & Sommerville, J. (1997). Xp54, the Xenopus homologue of human RNA helicase p54, is an integral component of stored mRNP particles in oocytes. Nucleic Acids Research, 25(5), 965-973

In investigating the composition of stored (maternal) mRNP particles in Xenopus oocytes, attention has focussed primarily on the phosphoproteins pp60/56, which are Y-box proteins involved in a general packaging of mRNA. We now identify a third, abund... Read More about Xp54, the Xenopus homologue of human RNA helicase p54, is an integral component of stored mRNP particles in oocytes.

Transcription and masking of mRNA in germ cells: Involvement of Y-box proteins (1996)
Journal Article
Sommerville, J., & Ladomery, M. (1996). Transcription and masking of mRNA in germ cells: Involvement of Y-box proteins. Chromosoma, 104(7), 469-478. https://doi.org/10.1007/BF00352111

Gametogenesis is directed by various specialized genetic mechanisms which, to a considerable extent, apply to the production of both eggs and sperm and have been conserved across a wide spectrum of eukaryotic organisms. Two key aspects which are disc... Read More about Transcription and masking of mRNA in germ cells: Involvement of Y-box proteins.

A role for Y‐box proteins in cell proliferation (1995)
Journal Article
Ladomery, M., & Sommerville, J. (1995). A role for Y‐box proteins in cell proliferation. BioEssays, 17(1), 9-11. https://doi.org/10.1002/bies.950170104

Members of the Y‐box (YB) family of transcription factors are expressed in a wide range of cell types and are implicated in the regulation of a rapidly increasing number of genes. Although the biological activities of YB proteins appear to be varied,... Read More about A role for Y‐box proteins in cell proliferation.

Binding of Y-box proteins to RNA: Involvement of different protein domains (1994)
Journal Article
Ladomery, M., & Sommerville, J. (1994). Binding of Y-box proteins to RNA: Involvement of different protein domains. Nucleic Acids Research, 22(25), 5582-5589

Eukaryotlc Y-box proteins are reported to interact with a wide variety of nucleic acid structures to act as transcription factors and mRNA masking proteins. The modular structure of Y-box proteins Includes a highly conserved N-termlnal cold-shock... Read More about Binding of Y-box proteins to RNA: Involvement of different protein domains.