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Development of a novel dual-enzyme screen-printed amperometric biosensor for the analysis of esterified fatty acids (2023)
Journal Article
Smart, A., Crew, A., Doran, O., & Hart, J. (2023). Development of a novel dual-enzyme screen-printed amperometric biosensor for the analysis of esterified fatty acids. Journal of Food Composition and Analysis, 122, Article 105488. https://doi.org/10.1016/j.jfca.2023.105488

This paper describes, for the first time, the development of a novel trilinolein biosensor based on the immobilisation of lipase and lipoxygenase (LOX) onto a cobalt phthalocyanine screen-printed carbon electrode (CoPC-SPCE) in conjunction with amper... Read More about Development of a novel dual-enzyme screen-printed amperometric biosensor for the analysis of esterified fatty acids.

Exploration of shrimp shell chitin as an agricultural biostimulant and its effects on soil microbial respiration (2022)
Presentation / Conference
Swaffield, A. R., Crew, A., & Bonnett, S. (2022, July). Exploration of shrimp shell chitin as an agricultural biostimulant and its effects on soil microbial respiration. Poster presented at 22nd World Congress of Soil Science, Glasgow, UK

Chitin (poly-(1→4)-β-N-acetyl-D-glucosamine) is a novel bio-stimulant derived from an inert by-product from sustainable fish production. This study observed the effect of chitin derived from shrimp shells on agricultural soil microorganism metabolism... Read More about Exploration of shrimp shell chitin as an agricultural biostimulant and its effects on soil microbial respiration.

Novel method for the measurement of erythrocyte acetylcholinesterase activity in the Yt blood group system using amperometry in conjunction with screen-printed carbon electrodes (2020)
Journal Article
Crew, A., Karamatic Crew, V., Bagelow, L., Walser, P., Thornton, N., & Hart, J. (2020). Novel method for the measurement of erythrocyte acetylcholinesterase activity in the Yt blood group system using amperometry in conjunction with screen-printed carbon electrodes. Vox Sanguinis, 115(S1), 271-271. https://doi.org/10.1111/vox.13031

Background: The five antigens of the Yt blood group system are located on the membrane GPI‐linked erythrocyte acetylcholinesterase (AChE‐E), which is implicated in a range of clinical issues, including sleep apnoea and neurotoxin poisoning. Conformat... Read More about Novel method for the measurement of erythrocyte acetylcholinesterase activity in the Yt blood group system using amperometry in conjunction with screen-printed carbon electrodes.

Studies towards the development of a novel, screen-printed carbon-based, biosensor for the measurement of polyunsaturated fatty acids (2020)
Journal Article
Smart, A., Hart, J., Crew, A., & Doran, O. (2020). Studies towards the development of a novel, screen-printed carbon-based, biosensor for the measurement of polyunsaturated fatty acids. Applied Sciences, 10(21), 1-13. https://doi.org/10.3390/app10217779

This paper describes the design, development and characterisation of an electrochemical biosensor for the measurement of linoleic and α-linolenic acid, as representative free polyunsaturated fatty acids (PUFAs), that may be implicated in food safety... Read More about Studies towards the development of a novel, screen-printed carbon-based, biosensor for the measurement of polyunsaturated fatty acids.

Screen-printed carbon based biosensors and their applications in agri-food safety (2020)
Journal Article
Smart, A., Crew, A., Pemberton, R., Hughes, G., Doran, O., & Hart, J. P. (2020). Screen-printed carbon based biosensors and their applications in agri-food safety. Trends in Analytical Chemistry, 127, Article 115898. https://doi.org/10.1016/j.trac.2020.115898

This review focuses on the ways in which screen-printed carbon electrodes have been tailored with different biorecognition elements, including enzymes, antibodies, and aptamers, often with other modifiers, such as mediators and nanoparticles, to prod... Read More about Screen-printed carbon based biosensors and their applications in agri-food safety.

Novel, rapid, low-cost screen-printed (bio)sensors for the direct analysis of boar taint compounds androstenone and skatole in porcine adipose tissue: Comparison with a high-resolution gas chromatographic method (2019)
Journal Article
Westmacott, K. L., Crew, A. P., Doran, O., & Hart, J. P. (2020). Novel, rapid, low-cost screen-printed (bio)sensors for the direct analysis of boar taint compounds androstenone and skatole in porcine adipose tissue: Comparison with a high-resolution gas chromatographic method. Biosensors and Bioelectronics, 150, Article 111837. https://doi.org/10.1016/j.bios.2019.111837

© 2019 Elsevier B.V. This is the first report on the fabrication, characterisation and application of an electrochemical (bio)sensor system for the simultaneous measurement of skatole and androstenone. A biosensor for androstenone was fabricated usin... Read More about Novel, rapid, low-cost screen-printed (bio)sensors for the direct analysis of boar taint compounds androstenone and skatole in porcine adipose tissue: Comparison with a high-resolution gas chromatographic method.

An electrocatalytic screen-printed amperometric sensor for the selective measurement of thiamine (Vitamin B1) in food supplements (2019)
Journal Article
Smart, A., Westmacott, K. L., Crew, A., Doran, O., & Hart, J. P. (2019). An electrocatalytic screen-printed amperometric sensor for the selective measurement of thiamine (Vitamin B1) in food supplements. Biosensors, 9(3), Article 98. https://doi.org/10.3390/bios9030098

An electrocatalytic screen-printed sensor has been investigated for the measurement of the biologically important biomolecule vitamin B1 (thiamine) for the first time in food supplements. Under basic conditions, the vitamin was converted to its elect... Read More about An electrocatalytic screen-printed amperometric sensor for the selective measurement of thiamine (Vitamin B1) in food supplements.

A novel electroanalytical approach to the measurement of B vitamins in food supplements based on screen-printed carbon sensors (2017)
Journal Article
Westmacott, K. L., Crew, A. P., Doran, O., & Hart, J. P. (2018). A novel electroanalytical approach to the measurement of B vitamins in food supplements based on screen-printed carbon sensors. Talanta, 181, 13-18. https://doi.org/10.1016/j.talanta.2017.12.074

© 2017 Elsevier B.V. This paper describes the development of a novel electrochemical assay for the measurement of water-soluble vitamins in food and pharmaceutical products. The optimum conditions for the determination of vitamin B1 (thiamine), B2 (r... Read More about A novel electroanalytical approach to the measurement of B vitamins in food supplements based on screen-printed carbon sensors.

Recent advances in the fabrication and application of screen-printed electrochemical (bio)sensors based on carbon materials for biomedical, agri-food and environmental analyses (2016)
Journal Article
Pemberton, R. M., Crew, A., Hughes, G., Westmacott, K., Honeychurch, K. C., Crew, A. P., …Hart, J. P. (2016). Recent advances in the fabrication and application of screen-printed electrochemical (bio)sensors based on carbon materials for biomedical, agri-food and environmental analyses. Biosensors, 6(4), 50. https://doi.org/10.3390/bios6040050

This review describes recent advances in the fabrication of electrochemical (bio)sensors based on screen-printing technology involving carbon materials and their application in biomedical, agri-food and environmental analyses. It will focus on the va... Read More about Recent advances in the fabrication and application of screen-printed electrochemical (bio)sensors based on carbon materials for biomedical, agri-food and environmental analyses.

Sensor and method for detecting androstenone or skatole in boar taint (2016)
Patent
Hart, J., Crew, A., McGuire, N., & Doran, O. (2016). Sensor and method for detecting androstenone or skatole in boar taint. EP2966441 A1

The present application is concerned with a sensor system for and a method of detecting, and preferably quantifying, androstenone (CAS Reg.No. 18339-16-7) and/or skatole (CAS Reg. No. 83-34-1), the chemicals associated with boar taint. In a preferred... Read More about Sensor and method for detecting androstenone or skatole in boar taint.

The redox behaviour of diazepam (valium) using a disposable screen-printed sensor and its determination in drinks using a novel adsorptive stripping voltammetric assay (2013)
Presentation / Conference
Honeychurch, K. C., Crew, A. P., Northall, H., Radbourne, S., Davies, O., Newman, S., & Hart, J. P. (2013, June). The redox behaviour of diazepam (valium) using a disposable screen-printed sensor and its determination in drinks using a novel adsorptive stripping voltammetric assay. Presented at ISE Satellite Student Regional Symposium: Great Western Electrochemistry Meeting, University of Bath, Bath, England

The redox behaviour of diazepam (Valium®) using a disposable screen-printed sensor and its determination in drinks using a novel adsorptive stripping voltammetric assay (2013)
Journal Article
Crew, A., Honeychurch, K. C., Crew, A. P., Northall, H., Radbourne, S., Davies, O., …Hart, J. P. (2013). The redox behaviour of diazepam (Valium®) using a disposable screen-printed sensor and its determination in drinks using a novel adsorptive stripping voltammetric assay. Talanta, 116, 300-307. https://doi.org/10.1016/j.talanta.2013.05.017

In this study we investigated the possibility of applying disposable electrochemical screen-printed carbon sensors for the rapid identification and quantitative determination of diazepam in beverages. This was achieved utilising a previously unreport... Read More about The redox behaviour of diazepam (Valium®) using a disposable screen-printed sensor and its determination in drinks using a novel adsorptive stripping voltammetric assay.

Voltammetric behaviour of lead at a microband screen-printed carbon electrode and its trace determination in plants by stripping voltammetry (2012)
Presentation / Conference
Phillips, R., Honeychurch, K. C., Crew, A. P., Davies, R., Willey, N., & Hart, J. P. (2012, September). Voltammetric behaviour of lead at a microband screen-printed carbon electrode and its trace determination in plants by stripping voltammetry. Poster presented at Electrochem 2012: Electrochemical Horizons, Trinity College, Dublin

Technology enabled engagement between employer and student to improve employability (2012)
Presentation / Conference
Crew, A., Mitchell, P., Georgiou, C., Horrocks, E., Stringer, P., & Morton, C. (2012, April). Technology enabled engagement between employer and student to improve employability. Paper presented at 9th ALDinHE Conference, University of Leeds, UK

The University of West of England have sought to engage with innovative ways to overcome a shortage of opportunities for undergraduate students studying analytical chemistry, to engage with employers. This 30 minute paper presentation will describe... Read More about Technology enabled engagement between employer and student to improve employability.

A screen-printed, amperometric biosensor array incorporated into a novel automated system for the simultaneous determination of organophosphate pesticides (2011)
Journal Article
Crew, A. P., Lonsdale, D., Byrd, N., Pittson, R., & Hart, J. P. (2011). A screen-printed, amperometric biosensor array incorporated into a novel automated system for the simultaneous determination of organophosphate pesticides. Biosensors and Bioelectronics, 26(6), 2847-2851. https://doi.org/10.1016/j.bios.2010.11.018

Organophosphate pesticides present serious risks to human and environmental health. A rapid reliable, economical and portable analytical system will be of great benefit in the detection and prevention of contamination. A biosensor array based on six... Read More about A screen-printed, amperometric biosensor array incorporated into a novel automated system for the simultaneous determination of organophosphate pesticides.

Development of an anodic stripping voltammetric assay, using a disposable mercury-free screen-printed carbon electrode, for the determination of zinc in human sweat (2008)
Journal Article
Cowell, D. C., Crew, A. P., & Hart, J. P. (2008). Development of an anodic stripping voltammetric assay, using a disposable mercury-free screen-printed carbon electrode, for the determination of zinc in human sweat. Talanta, 75(5), 1221-1226. https://doi.org/10.1016/j.talanta.2008.01.043

This paper reports on the development of a novel electrochemical assay for Zn2+ in human sweat, which involves the use of disposable screen-printed carbon electrodes (SPCEs). Initially, SPCEs were used in conjunction with cyclic voltammetry to study... Read More about Development of an anodic stripping voltammetric assay, using a disposable mercury-free screen-printed carbon electrode, for the determination of zinc in human sweat.

Chapter 23 Screen-printed electrochemical (bio)sensors in biomedical, environmental and industrial applications (2007)
Journal Article
Hart, J. P., Crew, A., Crouch, E., Honeychurch, K. C., & Pemberton, R. (2007). Chapter 23 Screen-printed electrochemical (bio)sensors in biomedical, environmental and industrial applications. https://doi.org/10.1016/S0166-526X%2806%2949023-1

This chapter describes a number of approaches for the fabrication of prototype screen-printed electrochemical (bio) sensors together with selected applications in the areas of biomedical, environmental and industrial analyses. Novel methods of immobi... Read More about Chapter 23 Screen-printed electrochemical (bio)sensors in biomedical, environmental and industrial applications.

Development of a novel electrochemical immuno-assay using a screen printed electrode for the determination of secretory immunoglobulin A in human sweat (2007)
Journal Article
Cowell, D. C., Crew, A. P., Alford, C., & Hart, J. P. (2007). Development of a novel electrochemical immuno-assay using a screen printed electrode for the determination of secretory immunoglobulin A in human sweat. Electrochimica Acta, 52(16), 5232-5237. https://doi.org/10.1016/j.electacta.2007.02.035

This paper reports on the steps involved in the development of a novel electrochemical immunoassay for secretory immunoglobulin A (sIgA) determinations in human sweat. This novel immunoassay involves an initial step whereby sweat sIgA is adsorbed ont... Read More about Development of a novel electrochemical immuno-assay using a screen printed electrode for the determination of secretory immunoglobulin A in human sweat.

A screen-printed, amperometric, biosensor array for the detection of organophosphate pesticides based on inhibition of wild type, and mutant acetylcholinesterases, from Drosophila melanogaster (2004)
Journal Article
Hart, J. P., Crew, A. P., Hart, J. P., Wedge, R., Marty, J. L., & Fournier, D. (2004). A screen-printed, amperometric, biosensor array for the detection of organophosphate pesticides based on inhibition of wild type, and mutant acetylcholinesterases, from Drosophila melanogaster. Analytical Letters, 37(8), 1601-1610. https://doi.org/10.1081/AL-120037590

Screen-printed carbon electrodes (SPCEs) modified with cobalt phthalocyanine (CoPC) have been used as base transducers in the construction of amperometric pesticide biosensors. Six individual biosensors were fabricated by depositing wildtype (WT) ace... Read More about A screen-printed, amperometric, biosensor array for the detection of organophosphate pesticides based on inhibition of wild type, and mutant acetylcholinesterases, from Drosophila melanogaster.

Some Recent Designs and Developments of Screen-Printed Carbon Electrochemical Sensors/Biosensors for Biomedical, Environmental, and Industrial Analyses (2004)
Journal Article
Hart, J. P., Crew, A. P., Crouch, E., Honeychurch, K. C., & Pemberton, R. (2004). Some Recent Designs and Developments of Screen-Printed Carbon Electrochemical Sensors/Biosensors for Biomedical, Environmental, and Industrial Analyses. Analytical Letters, 37(5), 789-830. https://doi.org/10.1081/AL-120030682

This review describes the design and fabrication of electrochemical sensors/biosensors based on screen-printing technology and their applications in pharmaceutical, biomedical, environmental, and industrial analyses. Specific emphasis is placed on na... Read More about Some Recent Designs and Developments of Screen-Printed Carbon Electrochemical Sensors/Biosensors for Biomedical, Environmental, and Industrial Analyses.

Nitrogen leaching losses under a less intensive farming and environment (LIFE) integrated system (2003)
Journal Article
Webster, C., Conway, J., Crew, A. P., & Goulding, K. (2003). Nitrogen leaching losses under a less intensive farming and environment (LIFE) integrated system. Soil Use and Management, 19(1), 36-44. https://doi.org/10.1111/j.1475-2743.2003.tb00277.x

Less Intensive Farming and Environment (LIFE) management is a form of integrated farming which aims to meet farming's economic and environmental requirements. We used a farm-scale LIFE demonstration to measure nitrogen (N) leaching losses over a 6 ye... Read More about Nitrogen leaching losses under a less intensive farming and environment (LIFE) integrated system.

Novel automated electrochemical system, based on a screen-printed amperometric biosensor array, for the simultaneous determination of organophosphate pesticides in environmental samples
Presentation / Conference
Crew, A. P., Pittson, K., Lonsdale, D., & Hart, J. P. Novel automated electrochemical system, based on a screen-printed amperometric biosensor array, for the simultaneous determination of organophosphate pesticides in environmental samples. Paper presented at Electrochemistry Group Faraday and Analytical Division, Royal Society of Chemistry, Electrochemical Technology Group, Society of Chemical Industry, University of Liverpool