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Differential effects of reduced protein diets on fatty acid composition and gene expression in muscle and subcutaneous adipose tissue of Alentejana purebred and Large White ×â Landrace ×â Pietrain crossbred pigs (2013)
Journal Article
Prates, J. A., Pires, V. M., Bessa, R. J., Madeira, M. S., Pires, V., Alfaia, C. M., …Prates, J. (2013). Differential effects of reduced protein diets on fatty acid composition and gene expression in muscle and subcutaneous adipose tissue of Alentejana purebred and Large White ×â Landrace ×â Pietrain crossbred pigs. British Journal of Nutrition, 110(02), 216-229. https://doi.org/10.1017/S0007114512004916

The present study assessed the effect of pig genotype (fatty v. lean) and dietary protein and lysine (Lys) levels (normal v. reduced) on intramuscular fat (IMF) content, subcutaneous adipose tissue (SAT) deposition, fatty acid composition and mRNA le... Read More about Differential effects of reduced protein diets on fatty acid composition and gene expression in muscle and subcutaneous adipose tissue of Alentejana purebred and Large White ×â Landrace ×â Pietrain crossbred pigs.

Trpm8 agonists modulate contraction of the pig urinary bladder (2013)
Journal Article
Parsons, B. A., Drake, M. J., Vahabi, B., Parsons, B. A., Doran, O., Rhodes, A., …Drake, M. (2013). Trpm8 agonists modulate contraction of the pig urinary bladder. Canadian Journal of Physiology and Pharmacology, 91(7), 503-509. https://doi.org/10.1139/cjpp-2012-0406

The transient receptor potential melastin-8 (TRPM8) channel is activated by the "cooling" compounds menthol and icilin. Pathophysiologically, it is implicated in the overactive bladder and bladder cooling reflex, but the activity of TRPM8 in normal b... Read More about Trpm8 agonists modulate contraction of the pig urinary bladder.

Use of whole blood for analysis of disease-associated biomarkers (2013)
Journal Article
McLeod, J., Pemberton, R. M., May, J. E., Pemberton, R., Hart, J. P., McLeod, J. D., …Doran, O. (2013). Use of whole blood for analysis of disease-associated biomarkers. Analytical Biochemistry, 437(1), 59-61. https://doi.org/10.1016/j.ab.2013.02.024

Analyses for diagnosis and monitoring of pathological conditions often rely on blood samples, partly due to relative ease of collection. However, many interfering substances largely preclude the use of whole blood itself, necessitating separation of... Read More about Use of whole blood for analysis of disease-associated biomarkers.

Boar taint: Challenges and opportunities (2013)
Presentation / Conference
Doran, O. (2013, March). Boar taint: Challenges and opportunities. Presented at British Pig Executive Board Workshop for UK Meat Industry, Peterborough, UK

Hepatic lipogenic enzyme expression in pigs is affected by selection for decreased backfat thickness at constant intramuscular fat content (2013)
Journal Article
Doran, O., Muñoz, R., Munoz, R., Estany, J., Tor, M., & Doran, O. (2013). Hepatic lipogenic enzyme expression in pigs is affected by selection for decreased backfat thickness at constant intramuscular fat content. Meat Science, 93(3), 746-751. https://doi.org/10.1016/j.meatsci.2012.11.045

This study investigated (i) whether genetic selection for decreased backfat thickness at constant intramuscular fat (IMF) affects the expression of lipogenic enzymes in pig liver and (ii) whether expression of the hepatic lipogenic enzymes is related... Read More about Hepatic lipogenic enzyme expression in pigs is affected by selection for decreased backfat thickness at constant intramuscular fat content.

Fatty acid composition and lipogenic enzyme protein expression in subcutaneous adipose tissue of male pigs vaccinated against boar taint, barrows, and entire boars (2013)
Journal Article
Mackay, J., Pearce, M. C., Thevasagayam, S., & Doran, O. (2013). Fatty acid composition and lipogenic enzyme protein expression in subcutaneous adipose tissue of male pigs vaccinated against boar taint, barrows, and entire boars. Journal of Animal Science, 91(1), 395-404. https://doi.org/10.2527/jas.2011-4685

Objectives of this study were to compare fatty acid composition of subcutaneous adipose tissue of entire boars, barrows, and male pigs vaccinated against boar taint with a vaccine containing a GnRH analogue-protein conjugate (Improvac, Pfizer Animal... Read More about Fatty acid composition and lipogenic enzyme protein expression in subcutaneous adipose tissue of male pigs vaccinated against boar taint, barrows, and entire boars.

Effect of pig breed and dietary protein level on selected fatty acids and stearoylcoenzyme A desaturase protein expression in longissimus muscle and subcutaneous fat (2013)
Journal Article
Doran, O., Jeronimo, E., Prates, J. A., Bessa, R. J., Hughes, R. A., Jeromino, E., …Doran, O. (2013). Effect of pig breed and dietary protein level on selected fatty acids and stearoylcoenzyme A desaturase protein expression in longissimus muscle and subcutaneous fat. Journal of Animal Science, 91(9), 4540-4546. https://doi.org/10.2527/jas.2012-5963

The aims of the study were 1) to investigate effects of a low protein diet on fatty acids content and composition of the LM and subcutaneous adipose tissue in 3 genetically diverse breeds, Large White × Landrace, Alentejano, and Bizaro, and 2) to det... Read More about Effect of pig breed and dietary protein level on selected fatty acids and stearoylcoenzyme A desaturase protein expression in longissimus muscle and subcutaneous fat.

Investigation of Testosterone, Androstenone, and Estradiol Metabolism in HepG2 Cells and Primary Culture Pig Hepatocytes and Their Effects on 17βHSD7 Gene Expression (2012)
Journal Article
Chen, G., Li, S., Dong, X., Bai, Y., Chen, A., Yang, S., …Doran, O. (2012). Investigation of Testosterone, Androstenone, and Estradiol Metabolism in HepG2 Cells and Primary Culture Pig Hepatocytes and Their Effects on 17βHSD7 Gene Expression. PLoS ONE, 7(12), e52255. https://doi.org/10.1371/journal.pone.0052255

Steroid metabolism is important in various species. The accumulation of androgen metabolite, androstenone, in pig adipose tissue is negatively associated with pork flavor, odour and makes the meat unfit for human consumption. The 17β-hydroxysteroid d... Read More about Investigation of Testosterone, Androstenone, and Estradiol Metabolism in HepG2 Cells and Primary Culture Pig Hepatocytes and Their Effects on 17βHSD7 Gene Expression.

Investigation of testosterone, androstenone, and estradiol metabolism in HepG2 cells and primary culture pig hepatocytes and their effects on 17βHSD7 gene expression (2012)
Journal Article
Chen, G., Li, S., Dong, X., Bai, Y., Chen, A., Yang, S., …Doran, O. (2012). Investigation of testosterone, androstenone, and estradiol metabolism in HepG2 cells and primary culture pig hepatocytes and their effects on 17βHSD7 gene expression. PLoS ONE, 7, e52255. https://doi.org/10.1371/journal.pone.0052255

Influence of oilseed supplement ranging in n-6/n-3 ratio on fatty acid composition and δ5-, δ6-desaturase protein expression in steer muscles (2012)
Journal Article
Pickova, J., Turner, T. D., Mitchell, A., Duynisveld, J., Pickiva, J., Doran, O., & McNiven, M. A. (2012). Influence of oilseed supplement ranging in n-6/n-3 ratio on fatty acid composition and δ5-, δ6-desaturase protein expression in steer muscles. animal, 6(12), 1973-1984. https://doi.org/10.1017/S1751731112000985

This study investigated effects of roasted or extruded oilseed supplementation ranging in n-6/n-3 ratios from 0.3 to 5.0 on the fatty acid composition and expression of delta-5 desaturase (δ5d) and δ6-desaturase (δ6d) protein in commercial steer chee... Read More about Influence of oilseed supplement ranging in n-6/n-3 ratio on fatty acid composition and δ5-, δ6-desaturase protein expression in steer muscles.

Expression of the hepatic skatole- and androstenone-metabolising enzymes in entire male pigs of two live weights (2012)
Journal Article
Zamaratskaia, G., Stefanovic, S., Lundstrom, K., & Doran, O. (2012). Expression of the hepatic skatole- and androstenone-metabolising enzymes in entire male pigs of two live weights. Livestock Science, 145(1-3), 124-130. https://doi.org/10.1016/j.livsci.2012.01.006

The aim of the present study was to investigate an in vivo relationship between increased levels of testicular steroids and protein expression of the key enzymes involved in hepatic metabolism of skatole (cytochrome P4502E1 and CYP2E1 and CYP2A) and... Read More about Expression of the hepatic skatole- and androstenone-metabolising enzymes in entire male pigs of two live weights.

Relationship between the hepatic SULT2A1 protein expression and backfat androstenone level in pigs of three breeds (2010)
Presentation / Conference
Panella-Riera, N., Mackay, J., Shevillon, P., Oliver, M. A., Bonneau, M., & Doran, O. (2010, August). Relationship between the hepatic SULT2A1 protein expression and backfat androstenone level in pigs of three breeds. Paper presented at 61st Annual Meeting of the European Association for Animal Production, Heraklion, Greece

Excessive accumulation of androstenone in pig adipose tissue contributes to the phenomenon of boar taint, an off-odour of some cooked pork. One of the reasons for high androstenone accumulation is a low rate of androstenone metabolism in pig liver. T... Read More about Relationship between the hepatic SULT2A1 protein expression and backfat androstenone level in pigs of three breeds.

Effect of dietary fatty acids on expression of lipogenic enzymes and fatty acid profile in tissues of bulls (2010)
Journal Article
Martin, J., Herdmann, A., Nuernberg, K., Martin, K., Nuernberg, G., & Doran, O. (2010). Effect of dietary fatty acids on expression of lipogenic enzymes and fatty acid profile in tissues of bulls. animal, 4(5), 755-762. https://doi.org/10.1017/S1751731110000431

This study investigated the effects of dietary linolenic acid (C18:3n-3) v. linoleic acid (C18:2n-6) on fatty acid composition and protein expression of key lipogenic enzymes, acetyl-CoA carboxylase (ACC), stearoyl-CoA desaturase (SCD) and delta 6 de... Read More about Effect of dietary fatty acids on expression of lipogenic enzymes and fatty acid profile in tissues of bulls.

Comparison of two derivatisation methods for conjugated linoleic acid isomer analysis by Ag+-HPLC/DAD in beef fat (2010)
Journal Article
Dance, L. J. E., Doran, O., Hallett, K., Dannenberger, D., Nuernberg, G., & Nuernberg, K. (2010). Comparison of two derivatisation methods for conjugated linoleic acid isomer analysis by Ag+-HPLC/DAD in beef fat. European Journal of Lipid Science and Technology, 112(2), 188-194. https://doi.org/10.1002/ejlt.200900052

Two different procedures for the methylation of conjugated linoleic acid isomers (CLA) were compared by silver ion (Ag+)-HPLC. The base-catalysed method followed by an acid-catalysed method (NaOCH3/ BF3), and base hydrolysis followed by methylation w... Read More about Comparison of two derivatisation methods for conjugated linoleic acid isomer analysis by Ag+-HPLC/DAD in beef fat.

Relationship between the expression of key lipogenic enzymes, fatty acid composition, and intramuscular fat content of Limousin and Aberdeen Angus cattle (2010)
Journal Article
Ward, R. E., Woodward, B., Otter, N., & Doran, O. (2010). Relationship between the expression of key lipogenic enzymes, fatty acid composition, and intramuscular fat content of Limousin and Aberdeen Angus cattle. Livestock Science, 127(1), 22-29. https://doi.org/10.1016/j.livsci.2009.09.005

The amount and fatty acid composition of intramuscular fat (IMF) are major factors in determining the nutritional value of beef. Understanding the mechanisms regulating IMF formation is important for designing strategies for manipulation of IMF quant... Read More about Relationship between the expression of key lipogenic enzymes, fatty acid composition, and intramuscular fat content of Limousin and Aberdeen Angus cattle.

Acetyl-CoA carboxylase and stearoyl-CoA desaturase protein expression in subcutaneous adipose tissue is reduced in pigs selected for decreased backfat thickness at constant intramuscular fat content (2009)
Journal Article
Cánovas, A., Estany, J., Tor, M., Pena, R. N., & Doran, O. (2009). Acetyl-CoA carboxylase and stearoyl-CoA desaturase protein expression in subcutaneous adipose tissue is reduced in pigs selected for decreased backfat thickness at constant intramuscular fat content. Journal of Animal Science, 87(12), 3905-3914. https://doi.org/10.2527/jas.2009-2091

The objectives of this study were 1) to determine whether selection toward less subcutaneous fat thickness at constant intramuscular fat content in pigs is related to tissue-specific changes in the expression of lipogenic enzymes acetyl-CoA carboxyla... Read More about Acetyl-CoA carboxylase and stearoyl-CoA desaturase protein expression in subcutaneous adipose tissue is reduced in pigs selected for decreased backfat thickness at constant intramuscular fat content.