All Outputs (4)

Influence of metals in the column or instrument on performance in hydrophilic interaction liquid chromatography (2021)
Journal Article
McCalley, D. V. (2022). Influence of metals in the column or instrument on performance in hydrophilic interaction liquid chromatography. Journal of Chromatography A, 1663, Article 462751. https://doi.org/10.1016/j.chroma.2021.462751

A method is proposed for measuring the relative contribution of extracolumn and column effects to the detrimental interactions which occur between metal-sensitive solutes and the complete HPLC system. The method involves the substitution of a length... Read More about Influence of metals in the column or instrument on performance in hydrophilic interaction liquid chromatography.

But my peaks are not Gaussian! Part 3: Physicochemical causes of peak tailing (2021)
Journal Article
Mccalley, D., & Stoll, D. R. (2021). But my peaks are not Gaussian! Part 3: Physicochemical causes of peak tailing. LCGC North America, 39(11), 526–533, 539

Although symmetric peaks with Gaussian shapes are predicted by models of the chromatographic process, "perfect peaks" are not observed often outside of textbooks. Several physico-chemical phenomena can lead to asymmetric peak shapes, including analyt... Read More about But my peaks are not Gaussian! Part 3: Physicochemical causes of peak tailing.

Alternative mobile phase additives for the characterization of protein biopharmaceuticals in liquid chromatography – Mass spectrometry (2021)
Journal Article
Lardeux, H., Duivelshof, B. L., Colas, O., Beck, A., McCalley, D. V., Guillarme, D., & D’Atri, V. (2021). Alternative mobile phase additives for the characterization of protein biopharmaceuticals in liquid chromatography – Mass spectrometry. Analytica Chimica Acta, 1156, Article 338347. https://doi.org/10.1016/j.aca.2021.338347

When analyzing large complex protein biopharmaceuticals, ion-pairing agents imparting low pH are widely used as mobile phase additives to improve the chromatographic performance. However, one of the most effective additives in RPLC and HILIC, trifluo... Read More about Alternative mobile phase additives for the characterization of protein biopharmaceuticals in liquid chromatography – Mass spectrometry.

Evaluation of a linear free energy relationship for the determination of the column void volume in hydrophilic interaction chromatography (2021)
Journal Article
McCalley, D. V. (2021). Evaluation of a linear free energy relationship for the determination of the column void volume in hydrophilic interaction chromatography. Journal of Chromatography A, 1638, Article 461849. https://doi.org/10.1016/j.chroma.2020.461849

The application of a linear free energy relationship (LFER) to a variety of hydrophilic interaction chromatography columns with different bonded ligands and pore sizes was studied in order to determine their void volume Vm. The method was based on th... Read More about Evaluation of a linear free energy relationship for the determination of the column void volume in hydrophilic interaction chromatography.