A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM)

Allen, T. D.; Rutherford, S. A.; Murray, S.; Sanderson, Helen; Gardiner, F.; Kiseleva, E.; Goldberg, M. W.; Drummond, S. P.

doi:10.1038/nprot.2007.137

All Outputs (2)

Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro-formed nuclei for Western blotting and scanning electron microscopy (SEM) (2007)
Journal Article
Allen, T. D., Rutherford, S. A., Murray, S., Sanderson, H. S., Gardiner, F., Kiseleva, E., …Drummond, S. P. (2007). Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro-formed nuclei for Western blotting and scanning electron microscopy (SEM). Nature Protocols, 2(5), 1173-1179. https://doi.org/10.1038/nprot.2007.138

This protocol details methods for the generation of cell-free extracts and DNA templates from the eggs and sperm chromatin, respectively, of the clawed toad Xenopus laevis. We have used this system with scanning electron microscopy (SEM), as detailed... Read More about Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro-formed nuclei for Western blotting and scanning electron microscopy (SEM).

A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM) (2007)
Journal Article
Allen, T. D., Rutherford, S. A., Murray, S., Sanderson, H., Gardiner, F., Kiseleva, E., …Drummond, S. P. (2007). A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM). Nature Protocols, 2(5), 1166-1172. https://doi.org/10.1038/nprot.2007.137

This protocol details methods for the isolation of oocyte nuclear envelopes (NEs) from the African clawed toad Xenopus laevis, immunogold labeling of component proteins and subsequent visualization by field-emission scanning electron microscopy (FESE... Read More about A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM).