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Crystal structures of human mitochondrial branched chain aminotransferase reaction intermediates: Ketimine and pyridoxamine phosphate forms (2002)
Journal Article
Yennawar, N. H., Conway, M. E., Yennawar, H. P., Farber, G. K., & Hutson, S. M. (2002). Crystal structures of human mitochondrial branched chain aminotransferase reaction intermediates: Ketimine and pyridoxamine phosphate forms. Biochemistry, 41(39), 11592-11601. https://doi.org/10.1021/bi020221c

The three-dimensional structures of the isoleucine ketimine and the pyridoxamine phosphate forms of human mitochondrial branched chain aminotransferase (hBCATm) have been determined crystallographically at 1.9 Å resolution. The hBCATm-catalyzed trans... Read More about Crystal structures of human mitochondrial branched chain aminotransferase reaction intermediates: Ketimine and pyridoxamine phosphate forms.

A continuous 96-well plate spectrophotometric assay for branched-chain amino acid aminotransferases (2002)
Journal Article
Cooper, A. J. L., Conway, M., & Hutson, S. M. (2002). A continuous 96-well plate spectrophotometric assay for branched-chain amino acid aminotransferases. Analytical Biochemistry, 308(1), 100-105. https://doi.org/10.1016/S0003-2697%2802%2900243-9

A new, continuous 96-well plate spectrophotometric assay for the branched-chain amino acid aminotransferases is described. Transamination of L-leucine with α-ketoglutarate results in formation of α-ketoisocaproate, which is reductively aminated back... Read More about A continuous 96-well plate spectrophotometric assay for branched-chain amino acid aminotransferases.

Identification of a peroxide-sensitive redox switch at the CXXC motif in the human mitochondrial branched chain aminotransferase (2002)
Journal Article
Conway, M. E., Yennawar, N., Wallin, R., Poole, L. B., & Hutson, S. M. (2002). Identification of a peroxide-sensitive redox switch at the CXXC motif in the human mitochondrial branched chain aminotransferase. Biochemistry, 41(29), 9070-9078. https://doi.org/10.1021/bi020200i

The human mitochondrial branched chain aminotransferase isoenzyme (hBCATm) must be stored in a reducing environment to remain active. Oxidation or labeling of hBCATm with sulfhydryl reagents results in enzyme inhibition. In this study, we investigate... Read More about Identification of a peroxide-sensitive redox switch at the CXXC motif in the human mitochondrial branched chain aminotransferase.