Skip to main content

Research Repository

Advanced Search

All Outputs (1)

An improved conjugation method for Pseudomonas syringae (2020)
Journal Article
Neale, H. C., Hulin, M. T., Harrison, R. J., Jackson, R. W., Mansfield, J. W., & Arnold, D. L. (2020). An improved conjugation method for Pseudomonas syringae. Journal of Microbiological Methods, 177, Article 106025. https://doi.org/10.1016/j.mimet.2020.106025

In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the requ... Read More about An improved conjugation method for Pseudomonas syringae.