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All Outputs (24)

WT1 activates transcription of the splice factor kinase SRPK1 gene in PC3 and K562 cancer cells in the absence of corepressor BASP1 (2020)
Journal Article
Belali, T., Wodi, C., Clark, B., Cheung, M. K., Craig, T. J., Wheway, G., …Ladomery, M. (2020). WT1 activates transcription of the splice factor kinase SRPK1 gene in PC3 and K562 cancer cells in the absence of corepressor BASP1. BBA - Gene Regulatory Mechanisms, 1863(12), Article 194642. https://doi.org/10.1016/j.bbagrm.2020.194642

Dysregulated alternative splicing plays a prominent role in all hallmarks of cancer. The splice factor kinase SRPK1 drives the activity of oncogenic splice factors such as SRSF1. SRSF1 in turn promotes the expression of splice isoforms that favour tu... Read More about WT1 activates transcription of the splice factor kinase SRPK1 gene in PC3 and K562 cancer cells in the absence of corepressor BASP1.

Mutation spectrum of PRPF31, genotype-phenotype correlation in retinitis pigmentosa, and opportunities for therapy (2020)
Journal Article
Wheway, G., Douglas, A., Baralle, D., & Guillot, E. (2020). Mutation spectrum of PRPF31, genotype-phenotype correlation in retinitis pigmentosa, and opportunities for therapy. Experimental Eye Research, 192, Article 107950. https://doi.org/10.1016/j.exer.2020.107950

Pathogenic variants in pre-messenger RNA (pre-mRNA) splicing factor 31, PRPF31, are the second most common genetic cause of autosomal dominant retinitis pigmentosa (adRP) in most populations. This remains a completely untreatable and incurable form o... Read More about Mutation spectrum of PRPF31, genotype-phenotype correlation in retinitis pigmentosa, and opportunities for therapy.

Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa (2018)
Journal Article
Buskin, A., Zhu, L., Chichagova, V., Basu, B., Mozaffari-Jovin, S., Dolan, D., …Lako, M. (2018). Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa. Nature Communications, 9(1), 4234. https://doi.org/10.1038/s41467-018-06448-y

© 2018, The Author(s). Mutations in pre-mRNA processing factors (PRPFs) cause autosomal-dominant retinitis pigmentosa (RP), but it is unclear why mutations in ubiquitously expressed genes cause non-syndromic retinal disease. Here, we generate transcr... Read More about Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa.

Development and biological evaluation of fluorophosphonate-modified hydroxyapatite for orthopaedic applications (2018)
Journal Article
Neary, G., Blom, A. W., Shiel, A. I., Wheway, G., & Mansell, J. P. (2018). Development and biological evaluation of fluorophosphonate-modified hydroxyapatite for orthopaedic applications. Journal of Materials Science: Materials in Medicine, 29(8), https://doi.org/10.1007/s10856-018-6130-9

© 2018, Springer Science+Business Media, LLC, part of Springer Nature. Abstract: There is an incentive to functionalise hydroxyapatite (HA) for orthopaedic implant use with bioactive agents to encourage superior integration of the implants into host... Read More about Development and biological evaluation of fluorophosphonate-modified hydroxyapatite for orthopaedic applications.

Signaling through the primary cilium (2018)
Journal Article
Wheway, G., Nazlamova, L., & Hancock, J. T. (2018). Signaling through the primary cilium. Frontiers in Cell and Developmental Biology, 6(FEB), https://doi.org/10.3389/fcell.2018.00008

© 2018 Wheway, Nazlamova and Hancock. The presence of single, non-motile "primary" cilia on the surface of epithelial cells has been well described since the 1960s. However, for decades these organelles were believed to be vestigial, with no remainin... Read More about Signaling through the primary cilium.

Meckel-Gruber syndrome: An update on diagnosis, clinical management, and research advances (2017)
Journal Article
Hartill, V., Szymanska, K., Sharif, S. M., Wheway, G., & Johnson, C. A. (2017). Meckel-Gruber syndrome: An update on diagnosis, clinical management, and research advances. Frontiers in Pediatrics, 5(244), 1-9. https://doi.org/10.3389/fped.2017.00244

© 2017 Hartill, Szymanska, Sharif, Wheway and Johnson. Meckel-Gruber syndrome (MKS) is a lethal autosomal recessive congenital anomaly syndrome caused by mutations in genes encoding proteins that are structural or functional components of the primary... Read More about Meckel-Gruber syndrome: An update on diagnosis, clinical management, and research advances.

Particle sorting by Paramecium cilia arrays (2017)
Journal Article
Whiting, J. G., Mayne, R., Wheway, G., Melhuish, C., & Adamatzky, A. (2017). Particle sorting by Paramecium cilia arrays. BioSystems, 156-157, 46-52. https://doi.org/10.1016/j.biosystems.2017.04.001

© 2017 Elsevier B.V. Motile cilia are cell-surface organelles whose purposes, in ciliated protists and certain ciliated metazoan epithelia, include generating fluid flow, sensing and substance uptake. Certain properties of cilia arrays, such as beati... Read More about Particle sorting by Paramecium cilia arrays.

A high-throughput genome-wide siRNA screen for ciliogenesis identifies new ciliary functional components and ciliopathy genes (2015)
Journal Article
Nguyen, T. M., Wheway, G., Szymanska, K., Wheway, G., Doherty, D., Schmidts, M., …Johnson, C. (2015). A high-throughput genome-wide siRNA screen for ciliogenesis identifies new ciliary functional components and ciliopathy genes. Cilia, 4(SUPPLEMENT 1), O12. https://doi.org/10.1186/2046-2530-4-S1-O12

Defects in primary cilium biogenesis underlie the ciliopathies, a growing group of genetic disorders. We describe the first whole genome siRNA-based reverse genetics screen for defects in biogenesis and/or maintenance of the primary cilium, obtaining... Read More about A high-throughput genome-wide siRNA screen for ciliogenesis identifies new ciliary functional components and ciliopathy genes.

The Meckel-Gruber syndrome protein TMEM67 controls basal body positioning and epithelial branching morphogenesis in mice via the non-canonical Wnt pathway (2015)
Journal Article
Abdelhamed, Z. A., Natarajan, S., Wheway, G., Inglehearn, C. F., Toomes, C., Johnson, C. A., & Jagger, D. J. (2015). The Meckel-Gruber syndrome protein TMEM67 controls basal body positioning and epithelial branching morphogenesis in mice via the non-canonical Wnt pathway. Disease Models and Mechanisms, 8(6), 527-541. https://doi.org/10.1242/dmm.019083

© 2015. Published by The Company of Biologists Ltd. Ciliopathies are a group of developmental disorders that manifest with multi-organ anomalies. Mutations in TMEM67 (MKS3) cause a range of human ciliopathies, including Meckel-Gruber and Joubert synd... Read More about The Meckel-Gruber syndrome protein TMEM67 controls basal body positioning and epithelial branching morphogenesis in mice via the non-canonical Wnt pathway.

An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes (2015)
Journal Article
Van Dam, T. J., Letteboer, S. J., Nguyen, T. M. T., Wheway, G., Schmidts, M., Mans, D. A., …Johnson, C. A. (2015). An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes. Nature Cell Biology, 17(8), 1074-1087. https://doi.org/10.1038/ncb3201

© 2015 Macmillan Publishers Limited. Defects in primary cilium biogenesis underlie the ciliopathies, a growing group of genetic disorders. We describe a whole-genome siRNA-based reverse genetics screen for defects in biogenesis and/or maintenance of... Read More about An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes.

Screen-based identification and validation of four new ion channels as regulators of renal ciliogenesis (2015)
Journal Article
Ouden, K. D., Van Balkom, B. W., Slaats, G. G., Wheway, G., Foletto, V., Szymanska, K., …Giles, R. H. (2015). Screen-based identification and validation of four new ion channels as regulators of renal ciliogenesis. Journal of Cell Science, 128(24), 4550-4559. https://doi.org/10.1242/jcs.176065

©2015. To investigate the contribution of ion channels to ciliogenesis, we carried out a small interfering RNA (siRNA)-based reverse genetics screen of all ion channels in the mouse genome in murine inner medullary collecting duct kidney cells. This... Read More about Screen-based identification and validation of four new ion channels as regulators of renal ciliogenesis.

Atmin is a transcriptional regulator of both lung morphogenesis and ciliogenesis (2014)
Journal Article
Stevens, J. L., Goggolidou, P., L. Stevens, J., Agueci, F., Keynton, J., Wheway, G., …Norris, D. P. (2014). Atmin is a transcriptional regulator of both lung morphogenesis and ciliogenesis. Development, 141(20), 3966-3977. https://doi.org/10.1242/dev.107755

© 2014. Initially identified in DNA damage repair, ATM-interactor (ATMIN) further functions as a transcriptional regulator of lung morphogenesis. Here we analyse three mouse mutants, Atmingpg6/gpg6, AtminH210Q/H210Q and Dynll1GT/GT, revealing how ATM... Read More about Atmin is a transcriptional regulator of both lung morphogenesis and ciliogenesis.

Loss-of-function mutations in MICU1 cause a brain and muscle disorder linked to primary alterations in mitochondrial calcium signaling (2014)
Journal Article
Duchen, M. R., Sewry, C. A., Den Dunnen, J. T., Niks, E. H., Santen, G. W., Morgan, J. E., …Sheridan, E. (2014). Loss-of-function mutations in MICU1 cause a brain and muscle disorder linked to primary alterations in mitochondrial calcium signaling. Nature Genetics, 46(2), 188-193. https://doi.org/10.1038/ng.2851

Mitochondrial Ca 2+ uptake has key roles in cell life and death. Physiological Ca 2+ signaling regulates aerobic metabolism, whereas pathological Ca 2+ overload triggers cell death. Mitochondrial Ca 2+ uptake is mediated by the Ca 2+ uniporter comple... Read More about Loss-of-function mutations in MICU1 cause a brain and muscle disorder linked to primary alterations in mitochondrial calcium signaling.

The role of primary cilia in the development and disease of the retina (2013)
Journal Article
Parry, D. A., Johnson, C. A., Wheway, G., Parry, D. A., & Johnson, C. A. (2014). The role of primary cilia in the development and disease of the retina. Organogenesis, 10(1), 69-85. https://doi.org/10.4161/org.26710

The normal development and function of photoreceptors is essential for eye health and visual acuity in vertebrates. Mutations in genes encoding proteins involved in photoreceptor development and function are associated with a suite of inherited retin... Read More about The role of primary cilia in the development and disease of the retina.

The SYSCILIA gold standard (SCGSv1) of known ciliary components and its applications within a systems biology consortium (2013)
Journal Article
Giles, R. H., Slaats, G. G., Van Dam, T. J., Huynen, M. A., van Dam, T. J., Wheway, G., …Giles, R. H. (2013). The SYSCILIA gold standard (SCGSv1) of known ciliary components and its applications within a systems biology consortium. Cilia, 2(1), 7. https://doi.org/10.1186/2046-2530-2-7

The multinational SYSCILIA consortium aims to gain a mechanistic understanding of the cilium. We utilize multiple parallel high-throughput (HTP) initiatives to develop predictive models of relationships between complex genotypes and variable phenotyp... Read More about The SYSCILIA gold standard (SCGSv1) of known ciliary components and its applications within a systems biology consortium.

Aberrant Wnt signalling and cellular over-proliferation in a novel mouse model of Meckel-Gruber syndrome (2013)
Journal Article
Wheway, G., Abdelhamed, Z., Natarajan, S., Toomes, C., Inglehearn, C., & Johnson, C. A. (2013). Aberrant Wnt signalling and cellular over-proliferation in a novel mouse model of Meckel-Gruber syndrome. Developmental Biology, 377(1), 55-66. https://doi.org/10.1016/j.ydbio.2013.02.015

Meckel-Gruber syndrome (MKS) is an embryonic lethal ciliopathy resulting from mutations in genes encoding proteins localising to the primary cilium. Mutations in the basal body protein MKS1 account for 7% of cases of MKS. The condition affects the de... Read More about Aberrant Wnt signalling and cellular over-proliferation in a novel mouse model of Meckel-Gruber syndrome.

Variable expressivity of ciliopathy neurological phenotypes that encompass Meckel - Gruber syndrome and Joubert syndrome is caused by complex de-regulated ciliogenesis, Shh and wnt signalling defects (2013)
Journal Article
Abdelhamed, Z. A., Wheway, G., Szymanska, K., Natarajan, S., Toomes, C., Inglehearn, C., & Johnson, C. A. (2013). Variable expressivity of ciliopathy neurological phenotypes that encompass Meckel - Gruber syndrome and Joubert syndrome is caused by complex de-regulated ciliogenesis, Shh and wnt signalling defects. Human Molecular Genetics, 22(7), 1358-1372. https://doi.org/10.1093/hmg/dds546

The ciliopathies are a group of heterogeneous diseases with considerable variations in phenotype for allelic conditions such as Meckel-Gruber syndrome (MKS) and Joubert syndrome (JBTS) even at the inter-individual level within families. In humans, mu... Read More about Variable expressivity of ciliopathy neurological phenotypes that encompass Meckel - Gruber syndrome and Joubert syndrome is caused by complex de-regulated ciliogenesis, Shh and wnt signalling defects.

Human Homolog of Drosophila Ariadne (HHARI) is a marker of cellular proliferation associated with nuclear bodies (2012)
Journal Article
Elmehdawi, F., Wheway, G., Szymanska, K., Adams, M., High, A. S., Johnson, C. A., & Robinson, P. A. (2013). Human Homolog of Drosophila Ariadne (HHARI) is a marker of cellular proliferation associated with nuclear bodies. Experimental Cell Research, 319(3), 161-172. https://doi.org/10.1016/j.yexcr.2012.10.002

HHARI (also known as ARIH1) is an ubiquitin-protein ligase and is the cognate of the E2, UbcH7 (UBE2L3). To establish a functional role for HHARI in cellular proliferation processes, we performed a reverse genetics screen that identified n=86/522 (16... Read More about Human Homolog of Drosophila Ariadne (HHARI) is a marker of cellular proliferation associated with nuclear bodies.

A meckelin-filamin a interaction mediates ciliogenesis (2012)
Journal Article
Adams, M., Simms, R. J., Abdelhamed, Z., Dawe, H. R., Szymanska, K., Logan, C. V., …Johnson, C. A. (2012). A meckelin-filamin a interaction mediates ciliogenesis. Human Molecular Genetics, 21(6), 1272-1286. https://doi.org/10.1093/hmg/ddr557

MKS3, encoding the transmembrane receptor meckelin, is mutated in Meckel-Gruber syndrome (MKS), an autosomal-recessive ciliopathy. Meckelin localizes to the primary cilium, basal body and elsewhere within the cell. Here, we found that the cytoplasmic... Read More about A meckelin-filamin a interaction mediates ciliogenesis.

High throughput high content reverse genetics visual screens of ciliogenesis and cilia maintenance (2012)
Journal Article
Szymanska, K., Wheway, G., Natarajan, S., Higgins, J., Adams, M., Tomlinson, D. C., & Johnson, C. A. (2012). High throughput high content reverse genetics visual screens of ciliogenesis and cilia maintenance. Cilia, 1(S1), P49. https://doi.org/10.1186/2046-2530-1-S1-P49

Cilia are small, hair-like structures occurring on the apical surface of most of vertebrate cells. Defects in cilia cause a range of developmental phenotypes grouped into conditions called ciliopathies. Our aim is to dissect the structure and functio... Read More about High throughput high content reverse genetics visual screens of ciliogenesis and cilia maintenance.