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An improved conjugation method for Pseudomonas syringae (2020)
Journal Article
Neale, H. C., Hulin, M. T., Harrison, R. J., Jackson, R. W., Mansfield, J. W., & Arnold, D. L. (2020). An improved conjugation method for Pseudomonas syringae. Journal of Microbiological Methods, 177, Article 106025. https://doi.org/10.1016/j.mimet.2020.106025

In order to achieve saturating transposon mutagenesis of the genome of plant pathogenic strains of Pseudomonas syringae we needed to improve plasmid conjugation frequency. Manipulation of the growth stage of donor and recipient cells allowed the requ... Read More about An improved conjugation method for Pseudomonas syringae.

Genotypic and phenotypic analyses reveal distinct population structures and ecotypes for sugar beet-associated Pseudomonas in Oxford and Auckland (2020)
Journal Article
Zhang, X., Ritchie, S. R., Chang, H., Arnold, D. L., Jackson, R. W., & Rainey, P. B. (2020). Genotypic and phenotypic analyses reveal distinct population structures and ecotypes for sugar beet-associated Pseudomonas in Oxford and Auckland. Ecology and Evolution, 10(12), 5963-5975. https://doi.org/10.1002/ece3.6334

Fluorescent pseudomonads represent one of the largest groups of bacteria inhabiting the surfaces of plants, but their genetic composition in planta is poorly understood. Here, we examined the population structure and diversity of fluorescent pseudomo... Read More about Genotypic and phenotypic analyses reveal distinct population structures and ecotypes for sugar beet-associated Pseudomonas in Oxford and Auckland.

Pseudomonas kirkiae sp. nov., a novel species isolated from oak in the United Kingdom, and phylogenetic considerations of the genera Pseudomonas, Azotobacter and Azomonas (2020)
Journal Article
Bueno-Gonzalez, V., Brady, C., Denman, S., Allainguillaume, J., & Arnold, D. (2020). Pseudomonas kirkiae sp. nov., a novel species isolated from oak in the United Kingdom, and phylogenetic considerations of the genera Pseudomonas, Azotobacter and Azomonas. International Journal of Systematic and Evolutionary Microbiology, 70(4), 2426-2434. https://doi.org/10.1099/ijsem.0.004055

As the current episode of Acute Oak Decline (AOD) continues to affect native British oak in the United Kingdom, ongoing isola-tions from symptomatic and healthy oak have yielded a large Pseudomonas species population. These strains could be divided i... Read More about Pseudomonas kirkiae sp. nov., a novel species isolated from oak in the United Kingdom, and phylogenetic considerations of the genera Pseudomonas, Azotobacter and Azomonas.

Pseudomonas daroniae sp. nov. and Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK (2019)
Journal Article
Bueno-Gonzalez, V., Brady, C., Denman, S., Plummer, S., Allainguillaume, J., & Arnold, D. (2019). Pseudomonas daroniae sp. nov. and Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK. International Journal of Systematic and Evolutionary Microbiology, 69(11), 3368-3376. https://doi.org/10.1099/ijsem.0.003615

Twenty-two cream-coloured bacterial strains were isolated from oak trees affected by acute oak decline (AOD) in Southern England. Isolates were Gram-negative, motile, slightly curved rods, aerobic, non-spore-forming, catalase positive and oxidase pos... Read More about Pseudomonas daroniae sp. nov. and Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK.

Pseudomonas syringae: Enterprising epiphyte and stealthy parasite (2019)
Journal Article
Arnold, D., & Preston, G. (2019). Pseudomonas syringae: Enterprising epiphyte and stealthy parasite. Microbiology, 165(3), 251-253. https://doi.org/10.1099/mic.0.000715

© 2019 The Authors. Pseudomonas syringae is best known as a plant pathogenic bacterium that causes diseases in a multitude of hosts, and it has been used as a model organism to understand the biology of plant disease. Pathogenic and non-pathogenic is... Read More about Pseudomonas syringae: Enterprising epiphyte and stealthy parasite.

Supercoiling of an excised genomic island represses effector gene expression to prevent activation of host resistance (2018)
Journal Article
Neale, H., Jackson, R., Preston, G., & Arnold, D. (2018). Supercoiling of an excised genomic island represses effector gene expression to prevent activation of host resistance. Molecular Microbiology, 110(3), 444-454. https://doi.org/10.1111/mmi.14111

© 2018 The Authors Molecular Microbiology Published by John Wiley & Sons Ltd. The plant pathogen Pseudomonas syringae pv. phaseolicola, which causes halo blight disease of beans, contains a 106kb genomic island PPHGI-1. PPHGI-1 carries a gene, avrP... Read More about Supercoiling of an excised genomic island represses effector gene expression to prevent activation of host resistance.

Suppression of the in vitro growth and development of Microdochium nivale by phosphite (2018)
Journal Article
Dempsey, J., Wilson, I. D., Spencer-Phillips, P., & Arnold, D. L. (2018). Suppression of the in vitro growth and development of Microdochium nivale by phosphite. Plant Pathology, 67(6), 1296-1306. https://doi.org/10.1111/ppa.12846

© 2018 British Society for Plant Pathology The ascomycete fungus Microdochium nivale is a major pathogen of many species of the gramineae. Control measures rely heavily on chemical fungicides, making alternative means of disease reduction desirable.... Read More about Suppression of the in vitro growth and development of Microdochium nivale by phosphite.

Coping with environmental eukaryotes; identification of pseudomonas syringae genes during the interaction with alternative hosts or predators (2018)
Journal Article
Dorati, F., Barrett, G. A., Sanchez-Contreras, M., Arseneault, T., José, M. S., Studholme, D. J., …Jackson, R. W. (2018). Coping with environmental eukaryotes; identification of pseudomonas syringae genes during the interaction with alternative hosts or predators. Microorganisms, 6(2), Article 32. https://doi.org/10.3390/microorganisms6020032

Understanding the molecular mechanisms underpinning the ecological success of plant pathogens is critical to develop strategies for controlling diseases and protecting crops. Recent observations have shown that plant pathogenic bacteria, particularly... Read More about Coping with environmental eukaryotes; identification of pseudomonas syringae genes during the interaction with alternative hosts or predators.

Taxonomy and identification of bacteria associated with acute oak decline (2017)
Journal Article
Brady, C., Arnold, D. L., McDonald, J., & Denman, S. (2017). Taxonomy and identification of bacteria associated with acute oak decline. World Journal of Microbiology and Biotechnology, 33(7), https://doi.org/10.1007/s11274-017-2296-4

© 2017, The Author(s). Acute oak decline (AOD) is a relatively newly described disorder affecting native oak species in Britain. Symptomatic trees are characterised by stem bleeds from vertical fissures, necrotic lesions in the live tissue beneath an... Read More about Taxonomy and identification of bacteria associated with acute oak decline.

Hydrogen sulfide causes excision of a genomic island in Pseudomonas syringae pv. phaseolicola (2017)
Journal Article
Neale, H., Deshappriya, N., Arnold, D. L., Wood, M. E., Whiteman, M., & Hancock, J. T. (2017). Hydrogen sulfide causes excision of a genomic island in Pseudomonas syringae pv. phaseolicola. European Journal of Plant Pathology, 149(4), 911-921. https://doi.org/10.1007/s10658-017-1240-7

© 2017, The Author(s). Hydrogen sulfide (H2S) is known to be an important signalling molecule in both animals and plants, despite its toxic nature. In plants it has been seen to control stomatal apertures, so altering the ability of bacteria to invad... Read More about Hydrogen sulfide causes excision of a genomic island in Pseudomonas syringae pv. phaseolicola.

Confocal microscopy reveals in planta dynamic interactions between pathogenic, avirulent and non-pathogenic Pseudomonas syringae strains (2017)
Journal Article
Beuzón, C. R., Ruiz-Albert, J., Mansfield, J. W., Corry, D. S., Rufián, J. S., Macho, A. P., & Arnold, D. L. (2018). Confocal microscopy reveals in planta dynamic interactions between pathogenic, avirulent and non-pathogenic Pseudomonas syringae strains. Molecular Plant Pathology, 19(3), 537-551. https://doi.org/10.1111/mpp.12539

© 2017 BSPP AND JOHN WILEY & SONS LTD Recent advances in genomics and single-cell analysis have demonstrated the extraordinary complexity reached by microbial populations within their hosts. Communities range from complex multispecies groups to hom... Read More about Confocal microscopy reveals in planta dynamic interactions between pathogenic, avirulent and non-pathogenic Pseudomonas syringae strains.

Multi-locus sequence typing of Escherichia coli isolates with acquired ampC genes and ampC promoter mutations (2016)
Journal Article
Lawrance, L. M., Moore, P. C., Lewis, J. A., Moore, P. C. L., Arnold, D. L., & Lawrance, L. (2016). Multi-locus sequence typing of Escherichia coli isolates with acquired ampC genes and ampC promoter mutations. Diagnostic Microbiology and Infectious Disease, 86(3), 265-267. https://doi.org/10.1016/j.diagmicrobio.2016.08.008

© 2016 Elsevier Inc. Multi-locus sequence typing was used to reveal a high degree of diversity amongst the E. coli isolates with AmpC plasmid genes, and a high prevalence of the −32 mutation present.

A low frequency persistent reservoir of a genomic island in a pathogen population ensures island survival and improves pathogen fitness in a susceptible host (2016)
Journal Article
Neale, H., Laister, R., Payne, J., Preston, G., Jackson, R., & Arnold, D. L. (2016). A low frequency persistent reservoir of a genomic island in a pathogen population ensures island survival and improves pathogen fitness in a susceptible host. Environmental Microbiology, 18(11), 4144-4152. https://doi.org/10.1111/1462-2920.13482

The co-evolution of bacterial plant pathogens and their hosts is a complex and dynamic process. Host resistance imposes stress on invading pathogens that can lead to changes in the bacterial genome enabling the pathogen to escape host resistance. We... Read More about A low frequency persistent reservoir of a genomic island in a pathogen population ensures island survival and improves pathogen fitness in a susceptible host.

Pseudomonas syringae Differentiates into Phenotypically Distinct Subpopulations During Colonization of a Plant Host (2016)
Journal Article
Rufián, J. S., Sánchez-Romero, M. A., López-Márquez, D., Macho, A. P., Mansfield, J. W., Arnold, D. L., …Beuzón, C. R. (2016). Pseudomonas syringae Differentiates into Phenotypically Distinct Subpopulations During Colonization of a Plant Host. Environmental Microbiology, 18(10), 3593-3605. https://doi.org/10.1111/1462-2920.13497

© 2016 Society for Applied Microbiology and John Wiley & Sons Ltd Bacterial microcolonies with heterogeneous sizes are formed during colonization of Phaseolus vulgaris by Pseudomonas syringae. Heterogeneous expression of structural and regulatory c... Read More about Pseudomonas syringae Differentiates into Phenotypically Distinct Subpopulations During Colonization of a Plant Host.

Early changes in apoplast composition associated with defence and disease in interactions between Phaseolus vulgaris and the halo blight pathogen Pseudomonas syringae Pv. phaseolicola (2016)
Journal Article
O'Leary, B. M., Neale, H. C., Geilfus, C., Jackson, R. W., Arnold, D. L., & Preston, G. M. (2016). Early changes in apoplast composition associated with defence and disease in interactions between Phaseolus vulgaris and the halo blight pathogen Pseudomonas syringae Pv. phaseolicola. Plant, Cell and Environment, 39(10), 2172-2184. https://doi.org/10.1111/pce.12770

© 2016 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd. The apoplast is the arena in which endophytic pathogens such as Pseudomonas syringae grow and interact with plant cells. Using metabolomic and ion analysis techniqu... Read More about Early changes in apoplast composition associated with defence and disease in interactions between Phaseolus vulgaris and the halo blight pathogen Pseudomonas syringae Pv. phaseolicola.

Rapid identification of bacteria associated with Acute Oak Decline by high-resolution melt analysis (2016)
Journal Article
Brady, C., Allainguillaume, J., Denman, S., & Arnold, D. (2016). Rapid identification of bacteria associated with Acute Oak Decline by high-resolution melt analysis. Letters in Applied Microbiology, 63(2), 89-95. https://doi.org/10.1111/lam.12593

© 2016 The Society for Applied Microbiology Two Gram-negative Enterobacteriaceae, Gibbsiella quercinecans and Brenneria goodwinii, are frequently isolated from oak suffering from Acute Oak Decline. These two species are difficult to identify based on... Read More about Rapid identification of bacteria associated with Acute Oak Decline by high-resolution melt analysis.

Gibbsiella papilionis Kim et al. 2013 is a later heterotypic synonym of gibbsiella dentisursi Saito et al. 2013 (2015)
Journal Article
Brady, C., Irisawa, T., Iino, T., Ohkuma, M., Arnold, D. L., & Denman, S. (2015). Gibbsiella papilionis Kim et al. 2013 is a later heterotypic synonym of gibbsiella dentisursi Saito et al. 2013. International Journal of Systematic and Evolutionary Microbiology, 65(12), 4788-4791. https://doi.org/10.1099/ijsem.0.000649

© 2015 IUMS. Synonymy of Gibbsiella dentisursi DSM 23818T (=NUM 1720T) and Gibbsiella papilionis JCM 18389T (=LEN33T) was suspected following multilocus sequence analysis (MLSA) of both type strains in a previous classification study, where they were... Read More about Gibbsiella papilionis Kim et al. 2013 is a later heterotypic synonym of gibbsiella dentisursi Saito et al. 2013.

The identification of genes important in pseudomonas syringae pv. phaseolicola plant colonisation using in vitro screening of transposon libraries (2015)
Journal Article
Manoharan, B., Neale, H. C., Hancock, J. T., Jackson, R. W., & Arnold, D. L. (2015). The identification of genes important in pseudomonas syringae pv. phaseolicola plant colonisation using in vitro screening of transposon libraries. PLoS ONE, 10(9), Article e0137355. https://doi.org/10.1371/journal.pone.0137355

© 2015 Manoharan et al. The bacterial plant pathogen Pseudomonas syringae pv. phaseolicola (Pph) colonises the surface of common bean plants before moving into the interior of plant tissue, via wounds and stomata. In the intercellular spaces the path... Read More about The identification of genes important in pseudomonas syringae pv. phaseolicola plant colonisation using in vitro screening of transposon libraries.

Chromosomal ampC mutations in cefpodoxime-resistant, ESBL-negative uropathogenic Escherichia coli (2015)
Journal Article
Lawrance, L. M., Arnold, D. L., Moore, P. C., Lewis, J. A., Moore, P. M., Arnold, D. L., & Lawrance, L. (2015). Chromosomal ampC mutations in cefpodoxime-resistant, ESBL-negative uropathogenic Escherichia coli. British Journal of Biomedical Science, 72(1), 7-11. https://doi.org/10.1080/09674845.2015.11666789

AmpC β-lactamase is an enzyme commonly produced by Escherichia coli that causes resistance to cephalosporins and penicillins. Enzyme production is controlled by the strength of the promoter encoded by the chromosomal ampC gene, with the level of prod... Read More about Chromosomal ampC mutations in cefpodoxime-resistant, ESBL-negative uropathogenic Escherichia coli.

Rahnella victoriana sp. nov., Rahnella bruchi sp. nov., Rahnella woolbedingensis sp. nov., classification of Rahnella genomospecies 2 and 3 as Rahnella variigena sp. nov. and Rahnella inusitata sp. nov., respectively and emended description of the genus Rahnella (2014)
Journal Article
Brady, C., Hunter, G., Kirk, S., Arnold, D. L., & Denman, S. (2014). Rahnella victoriana sp. nov., Rahnella bruchi sp. nov., Rahnella woolbedingensis sp. nov., classification of Rahnella genomospecies 2 and 3 as Rahnella variigena sp. nov. and Rahnella inusitata sp. nov., respectively and emended description of the genus Rahnella. Systematic and Applied Microbiology, 37(8), 545-552. https://doi.org/10.1016/j.syapm.2014.09.001

© 2014 Published by Elsevier GmbH. Isolations from oak symptomatic of Acute Oak Decline, alder and walnut log tissue, and buprestid beetles in 2009-2012 yielded 32 Gram-negative bacterial strains showing highest gyrB sequence similarity to Rahnella a... Read More about Rahnella victoriana sp. nov., Rahnella bruchi sp. nov., Rahnella woolbedingensis sp. nov., classification of Rahnella genomospecies 2 and 3 as Rahnella variigena sp. nov. and Rahnella inusitata sp. nov., respectively and emended description of the genus Rahnella.