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Investigation of Testosterone, Androstenone, and Estradiol Metabolism in HepG2 Cells and Primary Culture Pig Hepatocytes and Their Effects on 17βHSD7 Gene Expression (2012)
Journal Article
Chen, G., Li, S., Dong, X., Bai, Y., Chen, A., Yang, S., …Doran, O. (2012). Investigation of Testosterone, Androstenone, and Estradiol Metabolism in HepG2 Cells and Primary Culture Pig Hepatocytes and Their Effects on 17βHSD7 Gene Expression. PLoS ONE, 7(12), e52255. https://doi.org/10.1371/journal.pone.0052255

Steroid metabolism is important in various species. The accumulation of androgen metabolite, androstenone, in pig adipose tissue is negatively associated with pork flavor, odour and makes the meat unfit for human consumption. The 17β-hydroxysteroid d... Read More about Investigation of Testosterone, Androstenone, and Estradiol Metabolism in HepG2 Cells and Primary Culture Pig Hepatocytes and Their Effects on 17βHSD7 Gene Expression.

The effect of pressure and mobile phase velocity on the retention properties of small analytes and large biomolecules in ultra-high pressure liquid chromatography (2012)
Journal Article
Fekete, S., Veuthey, J. L., McCalley, D. V., & Guillarme, D. (2012). The effect of pressure and mobile phase velocity on the retention properties of small analytes and large biomolecules in ultra-high pressure liquid chromatography. Journal of Chromatography A, 1270, 127-138. https://doi.org/10.1016/j.chroma.2012.10.056

A possible complication of ultra-high pressure liquid chromatography (UHPLC) is related to the effect of pressure and mobile phase velocity on the retention properties of the analytes. In the present work, numerous model compounds have been selected... Read More about The effect of pressure and mobile phase velocity on the retention properties of small analytes and large biomolecules in ultra-high pressure liquid chromatography.

Investigation of testosterone, androstenone, and estradiol metabolism in HepG2 cells and primary culture pig hepatocytes and their effects on 17βHSD7 gene expression (2012)
Journal Article
Chen, G., Li, S., Dong, X., Bai, Y., Chen, A., Yang, S., …Doran, O. (2012). Investigation of testosterone, androstenone, and estradiol metabolism in HepG2 cells and primary culture pig hepatocytes and their effects on 17βHSD7 gene expression. PLoS ONE, 7, e52255. https://doi.org/10.1371/journal.pone.0052255

Influence of oilseed supplement ranging in n-6/n-3 ratio on fatty acid composition and δ5-, δ6-desaturase protein expression in steer muscles (2012)
Journal Article
Pickova, J., Turner, T. D., Mitchell, A., Duynisveld, J., Pickiva, J., Doran, O., & McNiven, M. A. (2012). Influence of oilseed supplement ranging in n-6/n-3 ratio on fatty acid composition and δ5-, δ6-desaturase protein expression in steer muscles. animal, 6(12), 1973-1984. https://doi.org/10.1017/S1751731112000985

This study investigated effects of roasted or extruded oilseed supplementation ranging in n-6/n-3 ratios from 0.3 to 5.0 on the fatty acid composition and expression of delta-5 desaturase (δ5d) and δ6-desaturase (δ6d) protein in commercial steer chee... Read More about Influence of oilseed supplement ranging in n-6/n-3 ratio on fatty acid composition and δ5-, δ6-desaturase protein expression in steer muscles.

Distribution of the branched chain aminotransferase proteins in the human brain and their role in glutamate regulation (2012)
Journal Article
Hull, J., Hindy, M. E., Kehoe, P. G., Chalmers, K., Love, S., & Conway, M. E. (2012). Distribution of the branched chain aminotransferase proteins in the human brain and their role in glutamate regulation. Journal of Neurochemistry, 123(6), 997-1009. https://doi.org/10.1111/jnc.12044

The branched chain aminotransferase enzymes (BCAT) serve as nitrogen donors for the production of 30% of de novo glutamate synthesis in rat brain. Despite the importance of this major metabolite and excitatory neurotransmitter, the distribution of BC... Read More about Distribution of the branched chain aminotransferase proteins in the human brain and their role in glutamate regulation.

Investigating mechanisms for non-linear genotoxic responses, and analysing their effects in binary combination (2012)
Journal Article
Jenkins, G. J. S., Doak, S. H., Thomas, A. D., Verma, J. R., Rees, B. J., Lewis, P. D., …Johnson, G. E. (2012). Investigating mechanisms for non-linear genotoxic responses, and analysing their effects in binary combination. Genes and Environment, 34(4), 179-185. https://doi.org/10.3123/jemsge.34.179

A recent shift by the scientific and regulatory community, towards accepting the existence of non-linear dose responses for certain DNA reactive genotoxic agents, has unveiled a myriad of questions regarding their biological basis. The mechanisms res... Read More about Investigating mechanisms for non-linear genotoxic responses, and analysing their effects in binary combination.

MyRIP interaction with MyoVa on secretory granules is controlled by the cAMP-PKA pathway (2012)
Journal Article
Váradi, A., Molnár, E., Hayward, K., Brozzi, F., Lajus, S., Diraison, F., …Varadi, A. (2012). MyRIP interaction with MyoVa on secretory granules is controlled by the cAMP-PKA pathway. Molecular Biology of the Cell, 23(22), 4444-4455. https://doi.org/10.1091/mbc.E12-05-0369

Myosin- and Rab-interacting protein (MyRIP), which belongs to the protein kinase A (PKA) - anchoring family, is implicated in hormone secretion. However, its mechanism of action is not fully elucidated. Here we investigate the role of MyRIP in myosin... Read More about MyRIP interaction with MyoVa on secretory granules is controlled by the cAMP-PKA pathway.

The chronoamperometric and voltammetric behaviour of glutathione at screen-printed carbon micro-band electrodes modified with cobalt phthalocyanine (2012)
Journal Article
Honeychurch, K. C., & Hart, J. P. (2012). The chronoamperometric and voltammetric behaviour of glutathione at screen-printed carbon micro-band electrodes modified with cobalt phthalocyanine. Advances in Analytical Chemistry, 2(5), 46-52. https://doi.org/10.5923/j.aac.20120205.01

Screen-printed carbon electrodes (SPCEs) incorporating the electrocatalyst cobalt phthalocyanine (CoPC) have been successfully used for the manufacture of microband electrodes for the determination of reduced glutathione (GSH). Initially investigati... Read More about The chronoamperometric and voltammetric behaviour of glutathione at screen-printed carbon micro-band electrodes modified with cobalt phthalocyanine.

Sleep specialists' opinion on sleep disorders and fitness to drive a car: The necessity of continued education (2012)
Journal Article
Verster, J. C., Mets, M. A., Alford, C., & Verster, J. (2012). Sleep specialists' opinion on sleep disorders and fitness to drive a car: The necessity of continued education. Industrial Health, 50(6), 499-508. https://doi.org/10.2486/indhealth.2012-0001

Whether patients with sleep disorders are fit to drive, and who should determine this, is a matter of debate. However, scientific literature is available on these topics to aid clinicians making these decisions. A survey was conducted to assess sleep... Read More about Sleep specialists' opinion on sleep disorders and fitness to drive a car: The necessity of continued education.

Robust feature extraction from impedimetric signals using wavelet packet decomposition with application to cytotoxicity testing (2012)
Journal Article
Luxton, R., Wraith, P., Nibouche, M., Kiely, J., Abdul Djawad, Y., Djawad, Y. A., …Luxton, R. W. (2012). Robust feature extraction from impedimetric signals using wavelet packet decomposition with application to cytotoxicity testing. IET Science Measurement and Technology, 6(6), 456-463. https://doi.org/10.1049/iet-smt.2012.0009

Impedance-based cytotoxicity testing provides a simple, real-time and non-invasive assay technique that interferes minimally with cell morphology and function. In this study, impedimetric measurements of ECV304 cells with increasing concentrations of... Read More about Robust feature extraction from impedimetric signals using wavelet packet decomposition with application to cytotoxicity testing.

BAG-1 suppresses expression of the key regulatory cytokine transforming growth factor β (TGF-β1) in colorectal tumour cells (2012)
Journal Article
Williams, A. C., Paraskeva, C., Townsend, P. A., Hague, A., Southern, S. L., Collard, T. J., …Williams, A. C. (2013). BAG-1 suppresses expression of the key regulatory cytokine transforming growth factor β (TGF-β1) in colorectal tumour cells. Oncogene, 32(38), 4490-4499. https://doi.org/10.1038/onc.2012.480

As colorectal cancer remains the second highest cause of cancer-related deaths in much of the industrialised world, identifying novel strategies to prevent colorectal tumour development remains an important challenge. BAG-1 is a multi-functional prot... Read More about BAG-1 suppresses expression of the key regulatory cytokine transforming growth factor β (TGF-β1) in colorectal tumour cells.

Human Homolog of Drosophila Ariadne (HHARI) is a marker of cellular proliferation associated with nuclear bodies (2012)
Journal Article
Elmehdawi, F., Wheway, G., Szymanska, K., Adams, M., High, A. S., Johnson, C. A., & Robinson, P. A. (2013). Human Homolog of Drosophila Ariadne (HHARI) is a marker of cellular proliferation associated with nuclear bodies. Experimental Cell Research, 319(3), 161-172. https://doi.org/10.1016/j.yexcr.2012.10.002

HHARI (also known as ARIH1) is an ubiquitin-protein ligase and is the cognate of the E2, UbcH7 (UBE2L3). To establish a functional role for HHARI in cellular proliferation processes, we performed a reverse genetics screen that identified n=86/522 (16... Read More about Human Homolog of Drosophila Ariadne (HHARI) is a marker of cellular proliferation associated with nuclear bodies.

Positive interactions can produce species-rich communities and increase species turnover through time (2012)
Journal Article
Ellwood, M. D., Wang, Y., Ellwood, M. D. F., Maestre, F. T., Yang, Z., Wang, G., & Chu, C. (2012). Positive interactions can produce species-rich communities and increase species turnover through time. Journal of Plant Ecology, 5(4), 417-421. https://doi.org/10.1093/jpe/rts005

Aims: The process of facilitation, where a species increases the survival, growth, and fitness of another species, is becoming increasingly recognized as a critical factor in shaping the structure of plant communities. This process is particularly im... Read More about Positive interactions can produce species-rich communities and increase species turnover through time.

An exploration of the collaborative processes of making theatre inspired by science (2012)
Journal Article
Dowell, E., & Weitkamp, E. (2012). An exploration of the collaborative processes of making theatre inspired by science. Public Understanding of Science, 21(7), 891-901. https://doi.org/10.1177/0963662510394278

This research examined the collaborative processes of making theatre inspired by science through the analysis of 16 semi-structured interviews with individual collaborators (eight theatre practitioners and eight scientists). Interviews explored exper... Read More about An exploration of the collaborative processes of making theatre inspired by science.

Banana production systems: Identification of alternative systems for more sustainable production (2012)
Journal Article
Bellamy, A. S. (2013). Banana production systems: Identification of alternative systems for more sustainable production. AMBIO: A Journal of the Human Environment, 42(3), 334-343. https://doi.org/10.1007/s13280-012-0341-y

Large-scale, monoculture production systems dependent on synthetic fertilizers and pesticides, increase yields, but are costly and have deleterious impacts on human health and the environment. This research investigates variations in banana productio... Read More about Banana production systems: Identification of alternative systems for more sustainable production.

MyRIP interaction with MyoVa on secretory granules is controlled by the cAMP-PKA pathway (2012)
Journal Article
Brozzi, F., Lajus, S., Diraison, F., Rajatileka, S., Hayward, K., Regazzi, R., …Varadi, A. (2012). MyRIP interaction with MyoVa on secretory granules is controlled by the cAMP-PKA pathway. Molecular Biology of the Cell, 23(22), 4444-4455. https://doi.org/10.1091/mbc.E12-05-0369

Myosin- and Rab-interacting protein (MyRIP), which belongs to the protein kinase A (PKA)–anchoring family, is implicated in hormone secretion. However, its mechanism of action is not fully elucidated. Here we investigate the role of MyRIP in myosin V... Read More about MyRIP interaction with MyoVa on secretory granules is controlled by the cAMP-PKA pathway.

Evaluation of a silver-based electrocatalyst for the determination of hydrogen peroxide formed via enzymatic oxidation (2012)
Journal Article
Smyth, M. R., Gonzalez-Macia, L., & Killard, A. (2012). Evaluation of a silver-based electrocatalyst for the determination of hydrogen peroxide formed via enzymatic oxidation. Talanta, 99, 989-996. https://doi.org/10.1016/j.talanta.2012.07.082

Silver paste electrodes modified with lyotropic phases formed from dodecyl benzenesulphonic acid and KCl were used as the reductant in the determination of the hydrogen peroxide released from the enzymatic reaction of glucose oxidase with glucose and... Read More about Evaluation of a silver-based electrocatalyst for the determination of hydrogen peroxide formed via enzymatic oxidation.

Development of a novel, physiologically relevant cytotoxicity model: Application to the study of chemotherapeutic damage to mesenchymal stromal cells (2012)
Journal Article
May, J. E., Morse, H. R., Xu, J., & Donaldson, C. (2012). Development of a novel, physiologically relevant cytotoxicity model: Application to the study of chemotherapeutic damage to mesenchymal stromal cells. Toxicology and Applied Pharmacology, 263(3), 374-389. https://doi.org/10.1016/j.taap.2012.07.013

There is an increasing need for development of physiologically relevant in-vitro models for testing toxicity, however determining toxic effects of agents which undergo extensive hepatic metabolism can be particularly challenging. If a source of such... Read More about Development of a novel, physiologically relevant cytotoxicity model: Application to the study of chemotherapeutic damage to mesenchymal stromal cells.