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BCAT-induced autophagy regulates Aβ load through an interdependence of redox state and PKC phosphorylation-implications in Alzheimer's disease (2020)
Journal Article
Harris, M., El Hindy, M., Usmari-Moraes, M., Hudd, F., Shafei, M., Dong, M., …Conway, M. E. (in press). BCAT-induced autophagy regulates Aβ load through an interdependence of redox state and PKC phosphorylation-implications in Alzheimer's disease. Free Radical Biology and Medicine, https://doi.org/10.1016...eeradbiomed.2020.01.019

Leucine, nutrient signal and substrate for the branched chain aminotransferase (BCAT) activates the mechanistic target of rapamycin (mTORC1) and regulates autophagic flux, mechanisms implicated in the pathogenesis of neurodegenerative conditions such... Read More about BCAT-induced autophagy regulates Aβ load through an interdependence of redox state and PKC phosphorylation-implications in Alzheimer's disease.

Equations to support redox experimentation (2019)
Book Chapter
Hancock, J. T., & Whiteman, M. Equations to support redox experimentation. In J. T. Hancock, & M. E. Conway (Eds.), Redox-Mediated Signal Transduction, (183-195). Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9463-2_15

© Springer Science+Business Media, LLC, part of Springer Nature 2019. Working with redox compounds needs to take into account the oxidation and reduction state of the compound under study. This redox state can be influenced by the media in which the... Read More about Equations to support redox experimentation.

Methods for the addition of redox compounds (2019)
Book Chapter
Hancock, J. T. Methods for the addition of redox compounds. In J. T. Hancock, & M. E. Conway (Eds.), Redox-Mediated Signal Transduction, (13-25). Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9463-2_2

© Springer Science+Business Media, LLC, part of Springer Nature 2019. Often in redox biology experiments there is a need to add compounds which impinge on the redox of the cellular environment cell. Such compounds may include reactive oxygen species... Read More about Methods for the addition of redox compounds.

Investigating ROS, RNS, and H2S-sensitive signaling proteins (2019)
Book Chapter
Williams, E., Whiteman, M., Wood, M. E., Wilson, I. D., Ladomery, M. R., Allainguillaume, J., …Hancock, J. T. (2019). Investigating ROS, RNS, and H2S-sensitive signaling proteins. In J. T. Hancock, & M. E. Conway (Eds.), Redox-Mediated Signal Transduction, (27-42). Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9463-2_3

© Springer Science+Business Media, LLC, part of Springer Nature 2019. The modification of proteins is a key way to alter their activity and function. Often thiols, cysteine residues, on proteins are attractive targets for such modification. Assuming... Read More about Investigating ROS, RNS, and H2S-sensitive signaling proteins.

Predicting the effects of low dose-rate ionizing radiation on redox potential in plant cells (2019)
Book Chapter
Caplin, N., & Willey, N. (2019). Predicting the effects of low dose-rate ionizing radiation on redox potential in plant cells. In J. T. Hancock, & M. E. Conway (Eds.), Redox-Mediated Signal Transduction, (135-142). New York, USA: Humana. https://doi.org/10.1007/978-1-4939-9463-2_11

During exposure of cells to acute high dose-rate ionizing radiation (IR), oxidants from the radiolysis of water can overwhelm antioxidant systems. Protecting flora from the effects of IR released from a nuclear industry of increasing global significa... Read More about Predicting the effects of low dose-rate ionizing radiation on redox potential in plant cells.

Novel Blood Biomarkers that Correlate with Cognitive Performance and Hippocampal Volumetry: Potential for Early Diagnosis of Alzheimer's Disease (2019)
Journal Article
Hudd, F., Shiel, A., Harris, M., Bowdler, P., McCann, B., Tsivos, D., …Conway, M. E. (2019). Novel Blood Biomarkers that Correlate with Cognitive Performance and Hippocampal Volumetry: Potential for Early Diagnosis of Alzheimer's Disease. Journal of Alzheimer's Disease, 67(3), 931-947. https://doi.org/10.3233/JAD-180879

© 2019 - IOS Press and the authors. All rights reserved. Background: Differential diagnosis of people presenting with mild cognitive impairment (MCI) that will progress to Alzheimer's disease (AD) remains clinically challenging. Current criteria used... Read More about Novel Blood Biomarkers that Correlate with Cognitive Performance and Hippocampal Volumetry: Potential for Early Diagnosis of Alzheimer's Disease.

The role of redox in signal transduction (2019)
Book Chapter
Hancock, J. T. (2019). The role of redox in signal transduction. In J. T. Hancock, & M. E. Conway (Eds.), Redox-Mediated Signal Transduction, (1-11). Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9463-2_1

© Springer Science+Business Media, LLC, part of Springer Nature 2019. It is the functioning of efficient cell signaling which is vital for the survival of cells, whether it is a simple prokaryote or a complex eukaryote, including both animals and pla... Read More about The role of redox in signal transduction.

Immunotherapeutic and pharmacological approaches for the treatment of Alzheimer’s disease (2018)
Journal Article
Usmari Moraes, M., Gaudet, T., Hull, J., & Conway, M. E. (2018). Immunotherapeutic and pharmacological approaches for the treatment of Alzheimer’s disease. Bioscience Horizons, 11, 1-7

The development of efficacious treatments targeting Alzheimer’s disease (AD) aetiology has been proved an extensive, time consuming task. Currently prescribed therapies, primarily acetylcholinesterase inhibitors such as donepezil and the NMDAreceptor... Read More about Immunotherapeutic and pharmacological approaches for the treatment of Alzheimer’s disease.

Hypoxia modulates the stem cell population and induces EMT in the MCF-10A breast epithelial cell line (2017)
Journal Article
Daly, C. S., Dean, S. J., Daly, C., Conway, M. E., Qualtrough, D., Shafei, M., & Flemban, A. (2018). Hypoxia modulates the stem cell population and induces EMT in the MCF-10A breast epithelial cell line. Oncology Reports, 39(2), 483-490. https://doi.org/10.3892/or.2017.6125

A common feature among pre-malignant lesions is the induction of hypoxia through increased cell propagation and reduced access to blood flow. Hypoxia in breast cancer has been associated with poor patient prognosis, resistance to chemotherapy and inc... Read More about Hypoxia modulates the stem cell population and induces EMT in the MCF-10A breast epithelial cell line.

Distribution of the branched-chain α-ketoacid dehydrogenase complex E1α subunit and glutamate dehydrogenase in the human brain and their role in neuro-metabolism (2017)
Journal Article
Hull, J., Usmari Moraes, M., Brookes, E., Love, S., & Conway, M. E. (2018). Distribution of the branched-chain α-ketoacid dehydrogenase complex E1α subunit and glutamate dehydrogenase in the human brain and their role in neuro-metabolism. Neurochemistry International, 112, 49-58. https://doi.org/10.1016/j.neuint.2017.10.014

© 2017 Glutamate is the major excitatory neurotransmitter of the central nervous system, with the branched-chain amino acids (BCAAs) acting as key nitrogen donors for de novo glutamate synthesis. Despite the importance of these major metabolites, the... Read More about Distribution of the branched-chain α-ketoacid dehydrogenase complex E1α subunit and glutamate dehydrogenase in the human brain and their role in neuro-metabolism.

The impact of ageing reveals distinct roles for human dentate gyrus and CA3 in pattern separation and object recognition memory (2017)
Journal Article
Dillon, S. E., Tsivos, D., Knight, M., McCann, B., Pennington, C., Shiel, A. I., …Coulthard, E. J. (2017). The impact of ageing reveals distinct roles for human dentate gyrus and CA3 in pattern separation and object recognition memory. Scientific Reports, 7(1), https://doi.org/10.1038/s41598-017-13853-8

© 2017 The Author(s). Both recognition of familiar objects and pattern separation, a process that orthogonalises overlapping events, are critical for effective memory. Evidence is emerging that human pattern separation requires dentate gyrus. Dentate... Read More about The impact of ageing reveals distinct roles for human dentate gyrus and CA3 in pattern separation and object recognition memory.

Evaluation of recombinant factor C assay for the detection of divergent lipopolysaccharide structural species and comparison with Limulus amebocyte lysate-based assays and a human monocyte activity assay (2017)
Journal Article
Jackson, S. K., Sattar, A. A., Abate, W., Sattar, A. A., Liu, J., Conway, M. E., & Jackson, S. K. (2017). Evaluation of recombinant factor C assay for the detection of divergent lipopolysaccharide structural species and comparison with Limulus amebocyte lysate-based assays and a human monocyte activity assay. Journal of Medical Microbiology, 66(7), 888-897. https://doi.org/10.1099/jmm.0.000510

© 2017 The Authors. Purpose. The Limulus amebocytelysate (LAL) assay is widely used for the screening of lipopolysaccharide (LPS) in parenteral pharmaceuticals. However, correlation of LPS in Gram-negative bacterial infections by LAL assay has been p... Read More about Evaluation of recombinant factor C assay for the detection of divergent lipopolysaccharide structural species and comparison with Limulus amebocyte lysate-based assays and a human monocyte activity assay.

Divergent metabolic regulation of autophagy and mTORC1-early events in Alzheimer's disease? (2017)
Journal Article
Harris, M., Shafei, M., & Conway, M. E. (2017). Divergent metabolic regulation of autophagy and mTORC1-early events in Alzheimer's disease?. Frontiers in Aging Neuroscience, 9(JUN), https://doi.org/10.3389/fnagi.2017.00173

© 2017 Shafei, Harris and Conway. Alzheimer's disease (AD) is a progressive disease associated with the production and deposition of amyloid β-peptide (Aβ) aggregates and neurofibrillary tangles, which lead to synaptic and neuronal damage. Reduced au... Read More about Divergent metabolic regulation of autophagy and mTORC1-early events in Alzheimer's disease?.

Enhanced task-related brain activation and resting perfusion in healthy older adults after chronic blueberry supplementation (2017)
Journal Article
Aboo-Bakkar, Z., Bowtell, J. L., Zainie, A., Conway, M. E., Adlam, A. L. R., & Fulford, J. (2017). Enhanced task-related brain activation and resting perfusion in healthy older adults after chronic blueberry supplementation. Applied Physiology, Nutrition, and Metabolism, 42(7), 773-779. https://doi.org/10.1139/apnm-2016-0550

© 2017, Canadian Science Publishing. All rights reserved. Blueberries are rich in flavonoids, which possess antioxidant and anti-inflammatory properties. High flavonoid intakes attenuate age-related cognitive decline, but data from human intervention... Read More about Enhanced task-related brain activation and resting perfusion in healthy older adults after chronic blueberry supplementation.

Decreased expression of the mitochondrial bcat protein correlates with improved patient survival in idh-wt gliomas (2016)
Journal Article
Conway, M. E., Hull, J., El Hindy, M., Taylor, S., El Amraoui, F., Paton-Thomas, C., …Kurian, K. (2016). Decreased expression of the mitochondrial bcat protein correlates with improved patient survival in idh-wt gliomas. Brain Pathology, 26(6), 789-791. https://doi.org/10.1111/bpa.12385

Background and research question: Gliomas represent 43% of all solid intracranial tumours, of which glioblastomas have the poorest prognosis. Recently, the human cytosolic branched-chain aminotransferase protein (hBCATc), which metabolises the branch... Read More about Decreased expression of the mitochondrial bcat protein correlates with improved patient survival in idh-wt gliomas.

Altered Expression of Human Mitochondrial Branched Chain Aminotransferase in Dementia with Lewy Bodies and Vascular Dementia (2016)
Journal Article
Hutson, S. M., Kehoe, P. G., Hull, J., Ashby, E. L., Ashby, E., Kierzkowska, M., …Conway, M. E. (2017). Altered Expression of Human Mitochondrial Branched Chain Aminotransferase in Dementia with Lewy Bodies and Vascular Dementia. Neurochemical Research, 42(1), 306-319. https://doi.org/10.1007/s11064-016-1855-7

© 2016, The Author(s). Cytosolic and mitochondrial human branched chain aminotransferase (hBCATc and hBCATm, respectively) play an integral role in brain glutamate metabolism. Regional increased levels of hBCATc in the CA1 and CA4 region of Alzheimer... Read More about Altered Expression of Human Mitochondrial Branched Chain Aminotransferase in Dementia with Lewy Bodies and Vascular Dementia.

New insights into the role of the branched-chain aminotransferase proteins in the human brain (2015)
Journal Article
Hull, J., Patel, V. B., Hutson, S. M., & Conway, M. E. (2015). New insights into the role of the branched-chain aminotransferase proteins in the human brain. Journal of Neuroscience Research, 93(7), 987-998. https://doi.org/10.1002/jnr.23558

The human cytosolic branched-chain aminotransferase (hBCATc) enzyme is strategically located in glutamatergic neurons, where it is thought to provide approximately 30% of de novo nitrogen for brain glutamate synthesis. In health, glutamate plays a do... Read More about New insights into the role of the branched-chain aminotransferase proteins in the human brain.

Regional increase in the expression of the BCAT proteins in Alzheimer's disease brain: Implications in glutamate toxicity (2015)
Journal Article
Hull, J., Patel, V., El Hindy, M., Lee, C., Odeleye, E., Hezwani, M., …Conway, M. E. (2015). Regional increase in the expression of the BCAT proteins in Alzheimer's disease brain: Implications in glutamate toxicity. Journal of Alzheimer's Disease, 45(3), 891-905. https://doi.org/10.3233/JAD-142970

BACKGROUND The human branched chain aminotransferases (hBCATm, mitochondrial and hBCATc, cytosolic) are major contributors to brain glutamate production. This excitatory neurotransmitter is thought to contribute to neurotoxicity in neurodegenerati... Read More about Regional increase in the expression of the BCAT proteins in Alzheimer's disease brain: Implications in glutamate toxicity.

S-nitrosylation of the thioredoxin-like domains of protein disulphide isomerase and its role in neurodegenerative conditions (2015)
Journal Article
Conway, M. E., & Harris, M. (2015). S-nitrosylation of the thioredoxin-like domains of protein disulphide isomerase and its role in neurodegenerative conditions. Frontiers in Chemistry, 3(27), https://doi.org/10.3389/fchem.2015.00027

Correct protein folding and inhibition of protein aggregation is facilitated by a cellular "quality control system" that engages a network of protein interactions including molecular chaperones and the ubiquitin proteasome system. Key chaperones invo... Read More about S-nitrosylation of the thioredoxin-like domains of protein disulphide isomerase and its role in neurodegenerative conditions.