Skip to main content

Research Repository

Advanced Search

All Outputs (66)

RAP55: Insights into an evolutionarily conserved protein family (2009)
Journal Article
Marnef, A., Sommerville, J., & Ladomery, M. (2009). RAP55: Insights into an evolutionarily conserved protein family. International Journal of Biochemistry and Cell Biology, 41(5), 977-981. https://doi.org/10.1016/j.biocel.2008.06.015

The RAP55 protein family is evolutionarily conserved in eukaryotes. Two highly conserved paralogues, RAP55A and RAP55B, exist in vertebrates; their functional properties and expression patterns remain to be compared. RAP55 proteins share multiple dom... Read More about RAP55: Insights into an evolutionarily conserved protein family.

Expression of pro- and anti-angiogenic isoforms of VEGF is differentially regulated by splicing and growth factors (2008)
Journal Article
Amin, E. M., Nowak, D. G., Woolard, J., Konopatskaya, O., Saleem, M. A., Churchill, A. J., …Bates, D. O. (2008). Expression of pro- and anti-angiogenic isoforms of VEGF is differentially regulated by splicing and growth factors. Journal of Cell Science, 121(20), 3487-3495. https://doi.org/10.1242/jcs.016410

Vascular endothelial growth factor A (VEGFA; hereafter referred to as VEGF) is a key regulator of physiological and pathological angiogenesis. Two families of VEGF isoforms are generated by alternate splice-site selection in the terminal exon. Proxim... Read More about Expression of pro- and anti-angiogenic isoforms of VEGF is differentially regulated by splicing and growth factors.

New insights into the function of the Wilms tumor suppressor gene WT1 in podocytes (2008)
Journal Article
Morrison, A. A., Viney, R. L., Saleem, M. A., & Ladomery, M. (2008). New insights into the function of the Wilms tumor suppressor gene WT1 in podocytes. AJP - Renal Physiology, 295(1), F12-F17. https://doi.org/10.1152/ajprenal.00597.2007

The Wilms tumor suppressor gene WT1 is essential for early urogenital development: homozygous mutations in WT1 result in embryonic lethality due to a failure in the development of kidneys and gonads. In the adult kidney, WT1 expression is limited to... Read More about New insights into the function of the Wilms tumor suppressor gene WT1 in podocytes.

The post-transcriptional roles of WT1, a multifunctional zinc-finger protein (2008)
Journal Article
Morrison, A. A., Viney, R. L., & Ladomery, M. (2008). The post-transcriptional roles of WT1, a multifunctional zinc-finger protein. Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1785(1), 55-62. https://doi.org/10.1016/j.bbcan.2007.10.002

WT1 was first described in 1990 as a tumour suppressor gene associated with Wilms tumour (nephroblastoma). It encodes a typical transcription factor with four C2-H2 zinc fingers in the C-terminus. However WT1 is surprisingly complex at multiple level... Read More about The post-transcriptional roles of WT1, a multifunctional zinc-finger protein.

Alternative splicing in angiogenesis: The vascular endothelial growth factor paradigm (2007)
Journal Article
Ladomery, M., Harper, S. J., & Bates, D. O. (2007). Alternative splicing in angiogenesis: The vascular endothelial growth factor paradigm. Cancer Letters, 249(2), 133-142. https://doi.org/10.1016/j.canlet.2006.08.015

Alternative splicing, first discovered in the 1970s, has emerged as one of the key generators of proteomic diversity. Not surprisingly, alternative splicing is increasingly linked to the etiology of cancer. This is illustrated by vascular endothelial... Read More about Alternative splicing in angiogenesis: The vascular endothelial growth factor paradigm.

A proteomic investigation of glomerular podocytes from a Denys-Drash Syndrome patient with a mutation in the Wilms tumour suppressor gene WT1 (2007)
Journal Article
Van Den Heuver, L. P., Viney, R. L., Morrison, A. A., Ni, L., Mathieson, P. W., Saleem, M. A., & Ladomery, M. (2007). A proteomic investigation of glomerular podocytes from a Denys-Drash Syndrome patient with a mutation in the Wilms tumour suppressor gene WT1. Proteomics, 7(5), 804-815. https://doi.org/10.1002/pmic.200600666

Glomerular podocytes are essential for blood filtration in the kidney underpinned by their unique cytoskeletal morphology. An increasing number of kidney diseases are being associated with key podocyte abnormalities. The Wilms tumour suppressor gene... Read More about A proteomic investigation of glomerular podocytes from a Denys-Drash Syndrome patient with a mutation in the Wilms tumour suppressor gene WT1.

Presence of WT1 in nuclear messenger RNP particles in the human acute myeloid leukemia cell lines HL60 and K562 (2006)
Journal Article
Morrison, A. A., & Ladomery, M. (2006). Presence of WT1 in nuclear messenger RNP particles in the human acute myeloid leukemia cell lines HL60 and K562. Cancer Letters, 244(1), 136-141. https://doi.org/10.1016/j.canlet.2005.12.018

The WT1 gene is a key player in acute myeloid leukaemia, in which it is frequently over-expressed. WT1 encodes a multifunctional zinc finger protein transcription factor, which also binds mRNA. Thus increasing evidence suggests that WT1 works both at... Read More about Presence of WT1 in nuclear messenger RNP particles in the human acute myeloid leukemia cell lines HL60 and K562.

WT1 interacts with the splicing protein RBM4 and regulates its ability to modulate alternative splicing in vivo (2006)
Journal Article
Markus, M. A., Heinrich, B., Raitskin, O., Adams, D. J., Mangs, H., Goy, C., …Morris, B. J. (2006). WT1 interacts with the splicing protein RBM4 and regulates its ability to modulate alternative splicing in vivo. Experimental Cell Research, 312(17), 3379-3388. https://doi.org/10.1016/j.yexcr.2006.07.008

Wilm's tumor protein 1 (WT1), a protein implicated in various cancers and developmental disorders, consists of two major isoforms: WT1(-KTS), a transcription factor, and WT1(+KTS), a post-transcriptional regulator that binds to RNA and can interact w... Read More about WT1 interacts with the splicing protein RBM4 and regulates its ability to modulate alternative splicing in vivo.

The Wilms tumour suppressor protein WT1 (+KTS isoform) binds alpha-actinin 1 mRNA via its zinc-finger domain (2006)
Journal Article
Morrison, A. A., Venables, J. P., Dellaire, G., & Ladomery, M. (2006). The Wilms tumour suppressor protein WT1 (+KTS isoform) binds alpha-actinin 1 mRNA via its zinc-finger domain. Biochemistry and Cell Biology, 84(5), 789-798. https://doi.org/10.1139/O06-065

Mutations in WT1 are associated with developmental syndromes that affect the urogenital system and neoplasms, including Wilms tumour, acute myeloid leukemia, and breast and prostate cancers. The WT1 protein belongs to the early growth response family... Read More about The Wilms tumour suppressor protein WT1 (+KTS isoform) binds alpha-actinin 1 mRNA via its zinc-finger domain.

The Wilms' tumor 1 (WT1) gene (+KTS isoform) functions with a CTE to enhance translation from an unspliced RNA with a retained intron (2006)
Journal Article
Hammarskjöld, M. L., Bor, Y. C., Swartz, J., Morrison, A., Rekosh, D., & Ladomery, M. (2006). The Wilms' tumor 1 (WT1) gene (+KTS isoform) functions with a CTE to enhance translation from an unspliced RNA with a retained intron. Genes and Development, 20(12), 1597-1608. https://doi.org/10.1101/gad.1402306

The Wilms' tumor 1 (WT1) gene plays an important role in mammalian urogenital development, and dysregulation of this gene is observed in many human cancers. Alternative splicing of WT1 RNA leads to the expression of two major protein isoforms, WT1(+K... Read More about The Wilms' tumor 1 (WT1) gene (+KTS isoform) functions with a CTE to enhance translation from an unspliced RNA with a retained intron.

Development of an siRNA-based method for repressing specific genes in renal organ culture and its use to show that the Wt1 tumour suppressor is required for nephron differentiation (2004)
Journal Article
Davies, J. A., Ladomery, M., Hohenstein, P., Michael, L., Shafe, A., Spraggon, L., & Hastie, N. (2004). Development of an siRNA-based method for repressing specific genes in renal organ culture and its use to show that the Wt1 tumour suppressor is required for nephron differentiation. Human Molecular Genetics, 13(2), 235-246. https://doi.org/10.1093/hmg/ddh015

Wt1 is a tumour suppressor gene, mutation of which is a cause of Wilms' tumour, a childhood renal nephroblastoma. Wt1 is expressed in a rich pattern during renal development suggesting that it acts at three stages: determination of the kidney area, t... Read More about Development of an siRNA-based method for repressing specific genes in renal organ culture and its use to show that the Wt1 tumour suppressor is required for nephron differentiation.

Murine Denys-Drash syndrome: Evidence of podocyte de-differentiation and systemic mediation of glomerulosclerosis (2003)
Journal Article
Hastie, N. D., Fleming, S., Patek, C. E., Miles, C. G., Bellamy, C. O., Ladomery, M., …Hooper, M. L. (2003). Murine Denys-Drash syndrome: Evidence of podocyte de-differentiation and systemic mediation of glomerulosclerosis. Human Molecular Genetics, 12(18), 2379-2394. https://doi.org/10.1093/hmg/ddg240

Denys-Drash syndrome (DDS) is caused by dominant mutations of the Wilms' tumour suppressor gene, WT1, and characterized by a nephropathy involving diffuse mesangial sclerosis, male pseudohermaphroditism and/ or Wilms' tumourigenesis. Previously, we r... Read More about Murine Denys-Drash syndrome: Evidence of podocyte de-differentiation and systemic mediation of glomerulosclerosis.

Expression in Xenopus oocytes shows that WT1 binds transcripts in vivo, with a central role for zinc finger one (2003)
Journal Article
Ladomery, M., Sommerville, J., Woolner, S., Slight, J., & Hastie, N. (2003). Expression in Xenopus oocytes shows that WT1 binds transcripts in vivo, with a central role for zinc finger one. Journal of Cell Science, 116(8), 1539-1549. https://doi.org/10.1242/jcs.00324

The Wilms' tumour suppressor gene WT1 encodes a protein involved in urogenital development and disease. The salient feature of WT1 is the presence of four 'Krüppel'-type C2-H2 zinc fingers in the C-terminus. Uniquely to WTI, an evolutionarily conserv... Read More about Expression in Xenopus oocytes shows that WT1 binds transcripts in vivo, with a central role for zinc finger one.

Multifunctional zinc finger proteins in development and disease (2002)
Journal Article
Ladomery, M., & Dellaire, G. (2002). Multifunctional zinc finger proteins in development and disease. Annals of Human Genetics, 66(5-6), 331-342. https://doi.org/10.1046/j.1469-1809.2002.00121.x

Post-transcriptional processes contribute significantly towards the generation of proteomic diversity. An increasing number of mutations have been described that affect genes encoding components of the post-transcriptional machinery. In particular, m... Read More about Multifunctional zinc finger proteins in development and disease.

4SR, a novel zinc-finger protein with SR-repeats, is expressed during early development of Xenopus (2000)
Journal Article
Ladomery, M., Marshall, R., Arif, L., & Sommerville, J. (2000). 4SR, a novel zinc-finger protein with SR-repeats, is expressed during early development of Xenopus. Gene, 256(1-2), 293-302. https://doi.org/10.1016/S0378-1119%2800%2900375-9

The protein C4SR contains two cysteine 4 (C 4) zinc-finger motifs at its amino terminus, a stretch of acidic residues in the middle and a series of serine-arginine (SR) repeats at its carboxyl terminus. A cDNA clone encoding the zinc-finger domain wa... Read More about 4SR, a novel zinc-finger protein with SR-repeats, is expressed during early development of Xenopus.

Presence of WT1, the Wilm's tumor suppressor gene product, in nuclear poly(A)+ ribonucleoprotein (1999)
Journal Article
Hastie, N. D., Ladomery, M. R., Ladomery, M., Slight, J., Mc Ghee, S., & Hastie, N. (1999). Presence of WT1, the Wilm's tumor suppressor gene product, in nuclear poly(A)+ ribonucleoprotein. Journal of Biological Chemistry, 274(51), 36520-36526. https://doi.org/10.1074/jbc.274.51.36520

The tumor suppressor gene WT1 encodes a zinc finger protein, which consists of four C-terminal C2-H2 zinc fingers of the Kruppel type, and at the N terminus a Q/P-rich trans-regulatory domain, both characteristic of transcription factors. However, re... Read More about Presence of WT1, the Wilm's tumor suppressor gene product, in nuclear poly(A)+ ribonucleoprotein.

Multiple roles for the Wilms' tumor suppressor, WT1 (1999)
Journal Article
Davies, R., Moore, A., Schedl, A., Bratt, E., Miyahawa, K., Ladomery, M., …Hastie, N. (1999). Multiple roles for the Wilms' tumor suppressor, WT1. Cancer Research, 59, 1747s-1750s

Wilms' tumor is a childhood kidney tumor that is a striking example of the way that cancer may arise through development gone awry. A proportion of these tumors develop as a result of the loss of function mutations in the Wilms' tumor suppressor gene... Read More about Multiple roles for the Wilms' tumor suppressor, WT1.

Xenopus HDm, a maternally expressed histone deacetylase, belongs to an ancient family of acetyl-metabolizing enzymes (1997)
Journal Article
Ladomery, M., Lyons, S., & Sommerville, J. (1997). Xenopus HDm, a maternally expressed histone deacetylase, belongs to an ancient family of acetyl-metabolizing enzymes. Gene, 198(1-2), 275-280. https://doi.org/10.1016/S0378-1119%2897%2900325-9

Modification of core histones can alter chromatin structure, facilitating the activation and repression of genes. A key example is the acetylation of N-terminal lysines of the core histones. Recently, the mammalian histone deacetylase HD1 was cloned... Read More about Xenopus HDm, a maternally expressed histone deacetylase, belongs to an ancient family of acetyl-metabolizing enzymes.

Multifunctional proteins suggest connections between transcriptional and post-transcriptional processes (1997)
Journal Article
Ladomery, M. (1997). Multifunctional proteins suggest connections between transcriptional and post-transcriptional processes. BioEssays, 19(10), 903-909. https://doi.org/10.1002/bies.950191010

Recent findings indicate that substantial cross-talk may exist between transcriptional and post-transcriptional processes. Firstly, there are suggestions that specific promoters influence the post-transcriptional fate of transcripts, pointing to comm... Read More about Multifunctional proteins suggest connections between transcriptional and post-transcriptional processes.

Xp54, the Xenopus homologue of human RNA helicase p54, is an integral component of stored mRNP particles in oocytes (1997)
Journal Article
Ladomery, M., Wade, E., & Sommerville, J. (1997). Xp54, the Xenopus homologue of human RNA helicase p54, is an integral component of stored mRNP particles in oocytes. Nucleic Acids Research, 25(5), 965-973

In investigating the composition of stored (maternal) mRNP particles in Xenopus oocytes, attention has focussed primarily on the phosphoproteins pp60/56, which are Y-box proteins involved in a general packaging of mRNA. We now identify a third, abund... Read More about Xp54, the Xenopus homologue of human RNA helicase p54, is an integral component of stored mRNP particles in oocytes.