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All Outputs (61)

Going circular: history, present, and future of circRNAs in cancer. (2023)
Journal Article
Pisignano, G., Michael, D. C., Pirlog, R., Visal, T. H., Ladomery, M., & Calin, G. A. (2023). Going circular: history, present, and future of circRNAs in cancer. Oncogene, 42, 2783–2800. https://doi.org/10.1038/s41388-023-02780-w

To date, thousands of highly abundant and conserved single-stranded RNA molecules shaped into ring structures (circRNAs) have been identified. CircRNAs are multifunctional molecules that have been shown to regulate gene expression transcriptionally a... Read More about Going circular: history, present, and future of circRNAs in cancer..

SPHINX-based combination therapy as a potential novel treatment strategy for acute myeloid leukaemia (2023)
Journal Article
Wodi, C., Belali, T., Morse, R., Porazinski, S., & Ladomery, M. (2023). SPHINX-based combination therapy as a potential novel treatment strategy for acute myeloid leukaemia. British Journal of Biomedical Science, 80, 11041. https://doi.org/10.3389/bjbs.2023.11041

Introduction: Dysregulated alternative splicing is a prominent feature of cancer. The inhibition and knockdown of the SR splice factor kinase SRPK1 reduces tumour growth in vivo. As a result several SPRK1 inhibitors are in development including SPHIN... Read More about SPHINX-based combination therapy as a potential novel treatment strategy for acute myeloid leukaemia.

CDC2-like (CLK) protein kinase inhibition as a novel targeted therapeutic strategy in prostate cancer (2021)
Journal Article
Uzor, S., Porazinski, S., Li, L., Clark, B., Ajiro, M., Iida, K., …Ladomery, M. (2021). CDC2-like (CLK) protein kinase inhibition as a novel targeted therapeutic strategy in prostate cancer. Scientific Reports, 11(1), Article 7963. https://doi.org/10.1038/s41598-021-86908-6

Dysregulation of alternative splicing is a feature of cancer, both in aetiology and progression. It occurs because of mutations in splice sites or sites that regulate splicing, or because of the altered expression and activity of splice factors and o... Read More about CDC2-like (CLK) protein kinase inhibition as a novel targeted therapeutic strategy in prostate cancer.

Epigenenetic regulation of alternative splicing: How lncRNAs tailor the message (2021)
Journal Article
Pisignano, G., & Ladomery, M. (2021). Epigenenetic regulation of alternative splicing: How lncRNAs tailor the message. Non-Coding RNA, 7(1), Article 21. https://doi.org/10.3390/ncrna7010021

Alternative splicing is a highly fine-tuned regulated process and one of the main drivers of proteomic diversity across eukaryotes. The vast majority of human multi-exon genes is alternatively spliced in a cell type-and tissue-specific manner, and de... Read More about Epigenenetic regulation of alternative splicing: How lncRNAs tailor the message.

WT1 activates transcription of the splice factor kinase SRPK1 gene in PC3 and K562 cancer cells in the absence of corepressor BASP1 (2020)
Journal Article
Belali, T., Wodi, C., Clark, B., Cheung, M. K., Craig, T. J., Wheway, G., …Ladomery, M. (2020). WT1 activates transcription of the splice factor kinase SRPK1 gene in PC3 and K562 cancer cells in the absence of corepressor BASP1. BBA - Gene Regulatory Mechanisms, 1863(12), Article 194642. https://doi.org/10.1016/j.bbagrm.2020.194642

Dysregulated alternative splicing plays a prominent role in all hallmarks of cancer. The splice factor kinase SRPK1 drives the activity of oncogenic splice factors such as SRSF1. SRSF1 in turn promotes the expression of splice isoforms that favour tu... Read More about WT1 activates transcription of the splice factor kinase SRPK1 gene in PC3 and K562 cancer cells in the absence of corepressor BASP1.

Targeting the ERG oncogene with splice-switching oligonucleotides as a novel therapeutic strategy in prostate cancer (2020)
Journal Article
Li, L., Hobson, L., Perry, L., Clark, B., Heavey, S., Haider, A., …Ladomery, M. (2020). Targeting the ERG oncogene with splice-switching oligonucleotides as a novel therapeutic strategy in prostate cancer. British Journal of Cancer, 123, 1024-1032. https://doi.org/10.1038/s41416-020-0951-2

Background: The ERG oncogene, a member of the ETS family of transcription factor encoding genes, is a genetic driver of prostate cancer. It is activated through a fusion with the androgen-responsive TMPRSS2 promoter in 50% of cases. There is therefor... Read More about Targeting the ERG oncogene with splice-switching oligonucleotides as a novel therapeutic strategy in prostate cancer.

APC and AXIN2 are promising biomarker candidates for the early detection of adenomas and hyperplastic polyps (2020)
Journal Article
Almond, S., Anaraki, F., Young, C., Ashrafian Bonab, M., Asadzadeh-Aghdaei, H., Rezvani, H., …Ladomery, M. (2020). APC and AXIN2 are promising biomarker candidates for the early detection of adenomas and hyperplastic polyps. Cancer Informatics, 19, 1-8. https://doi.org/10.1177/1176935120972383

Aberrant activation of the WNT/CTNNB1 pathway is notorious in colorectal cancer (CRC). Here, we demonstrate that the expression of specific and crucial WNT signaling pathway genes is linked to disease progression in colonic adenomatous (AP) and hyper... Read More about APC and AXIN2 are promising biomarker candidates for the early detection of adenomas and hyperplastic polyps.

Abscisic acid induced a negative geotropic response in dark-incubated Chlamydomonas reinhardtii (2019)
Journal Article
Al-Hijab, L., Gregg, A., Davies, R., Macdonald, H., Ladomery, M., & Wilson, I. (2019). Abscisic acid induced a negative geotropic response in dark-incubated Chlamydomonas reinhardtii. Scientific Reports, 9(1), Article 12063. https://doi.org/10.1038/s41598-019-48632-0

© 2019, The Author(s). The phytohormone abscisic acid (ABA) plays a role in stresses that alter plant water status and may also regulate root gravitropism and hydrotropism. ABA also exists in the aquatic algal progenitors of land plants, but other th... Read More about Abscisic acid induced a negative geotropic response in dark-incubated Chlamydomonas reinhardtii.

Phenethyl isothiocyanate hampers growth and progression of HER2-positive breast and ovarian carcinoma by targeting their stem cell compartment (2019)
Journal Article
Koschorke, A., Faraci, S., Giani, D., Chiodoni, C., Iorio, E., Canese, R., …Pupa, S. M. (2019). Phenethyl isothiocyanate hampers growth and progression of HER2-positive breast and ovarian carcinoma by targeting their stem cell compartment. Cellular Oncology, 42(6), 815-828. https://doi.org/10.1007/s13402-019-00464-w

© 2019, International Society for Cellular Oncology. Purpose: Isothiocyanates elicit anticancer effects by targeting cancer stem cells (CSCs). Here, we tested the antitumor activity of phenethyl-isothiocyanate (PEITC), either alone or in combination... Read More about Phenethyl isothiocyanate hampers growth and progression of HER2-positive breast and ovarian carcinoma by targeting their stem cell compartment.

Altered VEGF splicing isoform balance in tumor endothelium involves activation of splicing factors Srpk1 and Srsf1 by the Wilms’ tumor suppressor Wt1 (2019)
Journal Article
Ladomery, M. (2019). Altered VEGF splicing isoform balance in tumor endothelium involves activation of splicing factors Srpk1 and Srsf1 by the Wilms’ tumor suppressor Wt1. Cells, 8(1), 41. https://doi.org/10.339010.3390/cells8010041

Angiogenesis is one hallmark of cancer. Vascular endothelial growth factor (VEGF) is a known inducer of angiogenesis. Many patients benefit from antiangiogenic therapies, which however have limitations. Although VEGF is overexpressed in most tumors,... Read More about Altered VEGF splicing isoform balance in tumor endothelium involves activation of splicing factors Srpk1 and Srsf1 by the Wilms’ tumor suppressor Wt1.

The Evolutionarily Conserved Cassette Exon 7b Drives ERG's Oncogenic Properties (2018)
Journal Article
Jumbe, S., Porazinski, S., Oltean, S., Mansell, J., Vahabi, B., Wilson, I., & Ladomery, M. (2019). The Evolutionarily Conserved Cassette Exon 7b Drives ERG's Oncogenic Properties. Translational Oncology, 12(1), 134-142. https://doi.org/10.1016/j.tranon.2018.09.001

© 2018 The oncogene ERG encodes an ETS family transcription factor and is implicated in blood, vascular, and bone development and in prostate, blood, and bone cancer. The ERG gene is alternatively spliced; of particular interest is its cassette exon... Read More about The Evolutionarily Conserved Cassette Exon 7b Drives ERG's Oncogenic Properties.

Autoregulation of the human splice factor kinase CLK1 through exon skipping and intron retention (2018)
Journal Article
Uzor, S., Zorzou, P., Bowler, E., Porazinski, S., Wilson, I. D., & Ladomery, M. (2018). Autoregulation of the human splice factor kinase CLK1 through exon skipping and intron retention. Gene, 670, 46-54. https://doi.org/10.1016/j.gene.2018.05.095

© 2018 Elsevier B.V. Alternative splicing is a key process required for the regulation of gene expression in normal development and physiology. It is regulated by splice factors whose activities are in turn regulated by splice factor kinases and phos... Read More about Autoregulation of the human splice factor kinase CLK1 through exon skipping and intron retention.

Targeting splicing in prostate cancer (2018)
Journal Article
Antonopoulou, E., & Ladomery, M. (2018). Targeting splicing in prostate cancer. International Journal of Molecular Sciences, 19(5), 1287. https://doi.org/10.3390/ijms19051287

© 2018 by the authors. Licensee MDPI, Basel, Switzerland. Over 95% of human genes are alternatively spliced, expressing splice isoforms that often exhibit antagonistic functions. We describe genes whose alternative splicing has been linked to prostat... Read More about Targeting splicing in prostate cancer.

Hypoxia leads to significant changes in alternative splicing and elevated expression of CLK splice factor kinases in PC3 prostate cancer cells (2018)
Journal Article
Bowler, E., Porazinski, S., Uzor, S., Thibault, P., Durand, M., Lapointe, E., …Ladomery, M. (2018). Hypoxia leads to significant changes in alternative splicing and elevated expression of CLK splice factor kinases in PC3 prostate cancer cells. BMC Cancer, 18(1), 355. https://doi.org/10.1186/s12885-018-4227-7

© 2018 The Author(s). Background: Mounting evidence suggests that one of the ways that cells adapt to hypoxia is through alternative splicing. The aim of this study was firstly to examine the effect of hypoxia on the alternative splicing of cancer as... Read More about Hypoxia leads to significant changes in alternative splicing and elevated expression of CLK splice factor kinases in PC3 prostate cancer cells.

Alternative splicing in the hippo pathway-implications for disease and potential therapeutic targets (2018)
Journal Article
Porazinski, S., & Ladomery, M. (2018). Alternative splicing in the hippo pathway-implications for disease and potential therapeutic targets. Genes, 9(3), 161. https://doi.org/10.3390/genes9030161

© 2018 by the authors. Licensee MDPI, Basel, Switzerland. Alternative splicing is a well-studied gene regulatory mechanism that produces biological diversity by allowing the production of multiple protein isoforms from a single gene. An involvement o... Read More about Alternative splicing in the hippo pathway-implications for disease and potential therapeutic targets.

The oncogenic transcription factor ERG represses the transcription of the tumour suppressor gene PTEN in prostate cancer cells (2017)
Journal Article
Adamo, P., Porazinski, S., Rajatileka, S., Jumbe, S., Hagen, R., Cheung, M. K., …Ladomery, M. (2017). The oncogenic transcription factor ERG represses the transcription of the tumour suppressor gene PTEN in prostate cancer cells. Oncology Letters, 14(5), 5605-5610. https://doi.org/10.3892/ol.2017.6841

© 2017, Spandidos Publications. All rights reserved. The oncogene ETS-related gene (ERG) encodes a transcription factor with roles in the regulation of haematopoiesis, angiogenesis, vasculogenesis, inflammation, migration and invasion. The ERG oncoge... Read More about The oncogenic transcription factor ERG represses the transcription of the tumour suppressor gene PTEN in prostate cancer cells.

Residual ground-water levels of the neonicotinoid thiacloprid perturb chemosensing of Caenorhabditis elegans (2017)
Journal Article
Floyd, K. G., Hopewell, H., Burnell, D., Hancock, J. T., Allainguillaume, J., Ladomery, M., & Wilson, I. D. (2017). Residual ground-water levels of the neonicotinoid thiacloprid perturb chemosensing of Caenorhabditis elegans. Ecotoxicology, 26(7), 981-990. https://doi.org/10.1007/s10646-017-1826-z

© 2017, The Author(s). This study investigated the neurological effects of residual ground-water levels of thiaclopridon the non-target organism Caenorhabditis elegans. Nematodes treated with thiacloprid showed a dose-dependent and significantly incr... Read More about Residual ground-water levels of the neonicotinoid thiacloprid perturb chemosensing of Caenorhabditis elegans.

Insulin receptor isoform variations in prostate cancer cells (2016)
Journal Article
Perks, C. M., Zielinska, H. A., Wang, J., Jarrett, C., Frankow, A., Ladomery, M. R., …Holly, J. M. P. (2016). Insulin receptor isoform variations in prostate cancer cells. Frontiers in Endocrinology, 7(SEP), https://doi.org/10.3389/fendo.2016.00132

� 2016 Perks, Zielinska, Wang, Jarrett, Frankow, Ladomery, Bahl, Rhodes, Oxley and Holly. Men who develop prostate cancer (PCa) increasingly have one of the co-morbidities associated with a Western lifestyle that are characterized by hyperinsulinem... Read More about Insulin receptor isoform variations in prostate cancer cells.

Oligonucleotide-based systems: DNA, microRNAs, DNA/RNA aptamers (2016)
Journal Article
Jolly, P., Estrela, P., & Ladomery, M. (2016). Oligonucleotide-based systems: DNA, microRNAs, DNA/RNA aptamers. Essays in Biochemistry, 60(1), 27-35. https://doi.org/10.1042/EBC20150004

© 2016 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society. There are an increasing number of applications that have been developed for oligonucleotide-based biosensing systems in genetics and biomedicine. Oligonuc... Read More about Oligonucleotide-based systems: DNA, microRNAs, DNA/RNA aptamers.

The oncogene ERG: A key factor in prostate cancer (2016)
Journal Article
Ladomery, M. R., Adamo, P., Adamo, P., & Ladomery, M. (2016). The oncogene ERG: A key factor in prostate cancer. Oncogene, 35(4), 403-414. https://doi.org/10.1038/onc.2015.109

© 2016 Macmillan Publishers Limited All rights reserved. ETS-related gene (ERG) is a member of the E-26 transformation-specific (ETS) family of transcription factors with roles in development that include vasculogenesis, angiogenesis, haematopoiesis... Read More about The oncogene ERG: A key factor in prostate cancer.