All Outputs (125)

Development of a novel dual-enzyme screen-printed amperometric biosensor for the analysis of esterified fatty acids (2023)
Journal Article
Smart, A., Crew, A., Doran, O., & Hart, J. (2023). Development of a novel dual-enzyme screen-printed amperometric biosensor for the analysis of esterified fatty acids. Journal of Food Composition and Analysis, 122, Article 105488. https://doi.org/10.1016/j.jfca.2023.105488

This paper describes, for the first time, the development of a novel trilinolein biosensor based on the immobilisation of lipase and lipoxygenase (LOX) onto a cobalt phthalocyanine screen-printed carbon electrode (CoPC-SPCE) in conjunction with amper... Read More about Development of a novel dual-enzyme screen-printed amperometric biosensor for the analysis of esterified fatty acids.

Novel method for the measurement of erythrocyte acetylcholinesterase activity in the Yt blood group system using amperometry in conjunction with screen-printed carbon electrodes (2020)
Journal Article
Crew, A., Karamatic Crew, V., Bagelow, L., Walser, P., Thornton, N., & Hart, J. (2020). Novel method for the measurement of erythrocyte acetylcholinesterase activity in the Yt blood group system using amperometry in conjunction with screen-printed carbon electrodes. Vox Sanguinis, 115(S1), 271-271. https://doi.org/10.1111/vox.13031

Background: The five antigens of the Yt blood group system are located on the membrane GPI‐linked erythrocyte acetylcholinesterase (AChE‐E), which is implicated in a range of clinical issues, including sleep apnoea and neurotoxin poisoning. Conformat... Read More about Novel method for the measurement of erythrocyte acetylcholinesterase activity in the Yt blood group system using amperometry in conjunction with screen-printed carbon electrodes.

Studies towards the development of a novel, screen-printed carbon-based, biosensor for the measurement of polyunsaturated fatty acids (2020)
Journal Article
Smart, A., Hart, J., Crew, A., & Doran, O. (2020). Studies towards the development of a novel, screen-printed carbon-based, biosensor for the measurement of polyunsaturated fatty acids. Applied Sciences, 10(21), 1-13. https://doi.org/10.3390/app10217779

This paper describes the design, development and characterisation of an electrochemical biosensor for the measurement of linoleic and α-linolenic acid, as representative free polyunsaturated fatty acids (PUFAs), that may be implicated in food safety... Read More about Studies towards the development of a novel, screen-printed carbon-based, biosensor for the measurement of polyunsaturated fatty acids.

Screen-printed carbon based biosensors and their applications in agri-food safety (2020)
Journal Article
Smart, A., Crew, A., Pemberton, R., Hughes, G., Doran, O., & Hart, J. P. (2020). Screen-printed carbon based biosensors and their applications in agri-food safety. Trends in Analytical Chemistry, 127, Article 115898. https://doi.org/10.1016/j.trac.2020.115898

This review focuses on the ways in which screen-printed carbon electrodes have been tailored with different biorecognition elements, including enzymes, antibodies, and aptamers, often with other modifiers, such as mediators and nanoparticles, to prod... Read More about Screen-printed carbon based biosensors and their applications in agri-food safety.

Novel, rapid, low-cost screen-printed (bio)sensors for the direct analysis of boar taint compounds androstenone and skatole in porcine adipose tissue: Comparison with a high-resolution gas chromatographic method (2019)
Journal Article
Westmacott, K. L., Crew, A. P., Doran, O., & Hart, J. P. (2020). Novel, rapid, low-cost screen-printed (bio)sensors for the direct analysis of boar taint compounds androstenone and skatole in porcine adipose tissue: Comparison with a high-resolution gas chromatographic method. Biosensors and Bioelectronics, 150, Article 111837. https://doi.org/10.1016/j.bios.2019.111837

© 2019 Elsevier B.V. This is the first report on the fabrication, characterisation and application of an electrochemical (bio)sensor system for the simultaneous measurement of skatole and androstenone. A biosensor for androstenone was fabricated usin... Read More about Novel, rapid, low-cost screen-printed (bio)sensors for the direct analysis of boar taint compounds androstenone and skatole in porcine adipose tissue: Comparison with a high-resolution gas chromatographic method.

An electrocatalytic screen-printed amperometric sensor for the selective measurement of thiamine (Vitamin B1) in food supplements (2019)
Journal Article
Smart, A., Westmacott, K. L., Crew, A., Doran, O., & Hart, J. P. (2019). An electrocatalytic screen-printed amperometric sensor for the selective measurement of thiamine (Vitamin B1) in food supplements. Biosensors, 9(3), Article 98. https://doi.org/10.3390/bios9030098

An electrocatalytic screen-printed sensor has been investigated for the measurement of the biologically important biomolecule vitamin B1 (thiamine) for the first time in food supplements. Under basic conditions, the vitamin was converted to its elect... Read More about An electrocatalytic screen-printed amperometric sensor for the selective measurement of thiamine (Vitamin B1) in food supplements.

Fabrication of miniaturised screen-printed glucose biosensors, using a water-based ink, and the evaluation of their electrochemical behaviour (2018)
Journal Article
Hughes, G., Pemberton, R. . M., Nicholas, P., & Hart, J. P. (2018). Fabrication of miniaturised screen-printed glucose biosensors, using a water-based ink, and the evaluation of their electrochemical behaviour. Electroanalysis, 30(8), 1608-1612. https://doi.org/10.1002/elan.+201+800+104

This paper describes a simple, convenient approach to the fabrication of microband electrodes and microband biosensors based on screen printing technology. These devices were printed in a three-electrode configuration on one strip; a silver/silver ch... Read More about Fabrication of miniaturised screen-printed glucose biosensors, using a water-based ink, and the evaluation of their electrochemical behaviour.

A novel electroanalytical approach to the measurement of B vitamins in food supplements based on screen-printed carbon sensors (2017)
Journal Article
Westmacott, K. L., Crew, A. P., Doran, O., & Hart, J. P. (2018). A novel electroanalytical approach to the measurement of B vitamins in food supplements based on screen-printed carbon sensors. Talanta, 181, 13-18. https://doi.org/10.1016/j.talanta.2017.12.074

© 2017 Elsevier B.V. This paper describes the development of a novel electrochemical assay for the measurement of water-soluble vitamins in food and pharmaceutical products. The optimum conditions for the determination of vitamin B1 (thiamine), B2 (r... Read More about A novel electroanalytical approach to the measurement of B vitamins in food supplements based on screen-printed carbon sensors.

Development of a simple, low cost chronoamperometric assay for fructose based on a commercial graphite-nanoparticle modified screen-printed carbon electrode (2017)
Journal Article
Nicholas, P., Pittson, R., & Hart, J. P. (2018). Development of a simple, low cost chronoamperometric assay for fructose based on a commercial graphite-nanoparticle modified screen-printed carbon electrode. Food Chemistry, 241, 122-126. https://doi.org/10.1016/j.foodchem.2017.08.077

© 2017 Elsevier Ltd This paper describes the development of a simple, low cost chronoamperometric assay, for the measurement of fructose, using a graphite-nanoparticle modified screen-printed electrode (SPCE-G-COOH). Cyclic voltammetry showed that th... Read More about Development of a simple, low cost chronoamperometric assay for fructose based on a commercial graphite-nanoparticle modified screen-printed carbon electrode.

Alpha-synuclein ferrireductase activity is detectible in vivo, is altered in Parkinson's disease and increases the neurotoxicity of DOPAL (2017)
Journal Article
McDowall, J. S., Ntai, I., Honeychurch, K. C., Hart, J. P., Colin, P., Schneider, B. L., & Brown, D. R. (2017). Alpha-synuclein ferrireductase activity is detectible in vivo, is altered in Parkinson's disease and increases the neurotoxicity of DOPAL. Molecular and Cellular Neuroscience, 85, 1-11. https://doi.org/10.1016/j.mcn.2017.08.001

© 2017 Elsevier Inc. The normal cellular role of α-synuclein is of potential importance in understanding diseases in which an aggregated form of the protein has been implicated. A potential loss or change in the normal function of α-synuclein could p... Read More about Alpha-synuclein ferrireductase activity is detectible in vivo, is altered in Parkinson's disease and increases the neurotoxicity of DOPAL.

Recent advances in the fabrication and application of screen-printed electrochemical (bio)sensors based on carbon materials for biomedical, agri-food and environmental analyses (2016)
Journal Article
Pemberton, R. M., Crew, A., Hughes, G., Westmacott, K., Honeychurch, K. C., Crew, A. P., …Hart, J. P. (2016). Recent advances in the fabrication and application of screen-printed electrochemical (bio)sensors based on carbon materials for biomedical, agri-food and environmental analyses. Biosensors, 6(4), 50. https://doi.org/10.3390/bios6040050

This review describes recent advances in the fabrication of electrochemical (bio)sensors based on screen-printing technology involving carbon materials and their application in biomedical, agri-food and environmental analyses. It will focus on the va... Read More about Recent advances in the fabrication and application of screen-printed electrochemical (bio)sensors based on carbon materials for biomedical, agri-food and environmental analyses.

Voltammetric behaviour and determination of clonazepam using a disposable screen-printed sensor and its determination in serum and wine (2016)
Presentation / Conference
Honeychurch, K. C., Brooks, J., & Hart, J. P. (2016, August). Voltammetric behaviour and determination of clonazepam using a disposable screen-printed sensor and its determination in serum and wine. Presented at Electrochem 2016, Leicester, UK

Clonazepam Screen-printed Carbon Electrodes Cyclic Voltammetry Gas chromatography mass spectrometry Adsorptive Stripping Voltammetry Sample Preparation and Analysis Conclusions

Design and development of novel screen-printed microelectrode and microbiosensor arrays fabricated using ultrafast pulsed laser ablation (2016)
Journal Article
Hart, J. P., Liébana, S., Jones, L. J., Drago, G. A., Pittson, R. W., Liu, D., …Hart, J. P. (2016). Design and development of novel screen-printed microelectrode and microbiosensor arrays fabricated using ultrafast pulsed laser ablation. Sensors and Actuators B: Chemical, 231, 384-392. https://doi.org/10.1016/j.snb.2016.02.142

© 2016 Elsevier B.V. All rights reserved. A new generic platform for the development of microbiosensors combining screen-printing and ultrafast pulsed laser technologies has been developed, characterised and evaluated. This new platform consists of a... Read More about Design and development of novel screen-printed microelectrode and microbiosensor arrays fabricated using ultrafast pulsed laser ablation.

A novel reagentless glutamate microband biosensor for real-time cell toxicity monitoring (2016)
Journal Article
Hart, J. P., Pemberton, R. M., Hughes, G., Hughes, G., Pemberton, R., Fielden, P. R., & Hart, J. P. (2016). A novel reagentless glutamate microband biosensor for real-time cell toxicity monitoring. Analytica Chimica Acta, 933, 82-88. https://doi.org/10.1016/j.aca.2016.06.005

© 2016 Elsevier B.V. A reagentless glutamate biosensor was applied to the determination of glutamate released from liver hepatocellular carcinoma cells (HepG2) in response to toxic challenge from various concentrations of paracetamol. A screen printe... Read More about A novel reagentless glutamate microband biosensor for real-time cell toxicity monitoring.

Amperometric Screen-Printed Galactose Biosensor for Cell Toxicity Applications (2016)
Journal Article
Jackson, S. K., Pemberton, R. M., Kanyong, P., Hughes, G., Pemberton, R., Jackson, S., & Hart, J. P. (2016). Amperometric Screen-Printed Galactose Biosensor for Cell Toxicity Applications. Analytical Letters, 49(2), 236-244. https://doi.org/10.1080/00032719.2015.1070166

© 2016, Copyright © Taylor & Francis Group, LLC. ABSTRACT: This paper reports the development and application of a biosensor for the amperometric determination of galactose in the presence of human hepatocellular carcinoma cells with and without a... Read More about Amperometric Screen-Printed Galactose Biosensor for Cell Toxicity Applications.

Sensor and method for detecting androstenone or skatole in boar taint (2016)
Patent
Hart, J., Crew, A., McGuire, N., & Doran, O. (2016). Sensor and method for detecting androstenone or skatole in boar taint. EP2966441 A1

The present application is concerned with a sensor system for and a method of detecting, and preferably quantifying, androstenone (CAS Reg.No. 18339-16-7) and/or skatole (CAS Reg. No. 83-34-1), the chemicals associated with boar taint. In a preferred... Read More about Sensor and method for detecting androstenone or skatole in boar taint.

The design, development and application of electrochemical glutamate biosensors (2015)
Journal Article
Hart, J. P., Pemberton, R. M., Hughes, G., Hughes, G., Pemberton, R., Fielden, P. R., & Hart, J. P. (2016). The design, development and application of electrochemical glutamate biosensors. Trends in Analytical Chemistry, 79, 106-113. https://doi.org/10.1016/j.trac.2015.10.020

© 2015 Elsevier B.V. The development of biosensors for the determination of glutamate has been of great research interest for the past 25 years due to its importance in biomedical and food studies. This review focusses on the various strategies used... Read More about The design, development and application of electrochemical glutamate biosensors.

Development of a voltammetric assay, using screen-printed electrodes, for clonazepam and its application to beverage and serum samples (2015)
Journal Article
Honeychurch, K. C., Brooks, J., & Hart, J. P. (2016). Development of a voltammetric assay, using screen-printed electrodes, for clonazepam and its application to beverage and serum samples. Talanta, 147, 510-515. https://doi.org/10.1016/j.talanta.2015.10.032

© 2015 Elsevier B.V. All rights reserved. This paper describes the development of an electrochemical assay based on screen-printed carbon sensors for the determination of clonazepam in serum and in wine. The cyclic voltammetric behaviour of the drug... Read More about Development of a voltammetric assay, using screen-printed electrodes, for clonazepam and its application to beverage and serum samples.

Development of a novel reagentless, screen-printed amperometric biosensor based on glutamate dehydrogenase and NAD+, integrated with multi-walled carbon nanotubes for the determination of glutamate in food and clinical applications (2015)
Journal Article
Hughes, G., Pemberton, R. M., Fielden, P. R., & Hart, J. P. (2015). Development of a novel reagentless, screen-printed amperometric biosensor based on glutamate dehydrogenase and NAD+, integrated with multi-walled carbon nanotubes for the determination of glutamate in food and clinical applications. Sensors and Actuators B: Chemical, 216, 614-621. https://doi.org/10.1016/j.snb.2015.04.066

© 2015 Elsevier B.V. Abstract A screen printed carbon electrode (SPCE) containing the electrocatalyst Meldola's Blue (MB) has been investigated as the base transducer for a reagentless glutamate biosensor. The biopolymer chitosan (CHIT) and multiwall... Read More about Development of a novel reagentless, screen-printed amperometric biosensor based on glutamate dehydrogenase and NAD+, integrated with multi-walled carbon nanotubes for the determination of glutamate in food and clinical applications.

Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers (2014)
Journal Article
Sage, I. C., Drago, G. A., Pemberton, R., Cox, T., Tuffin, R., Griffiths, J., …Hart, J. P. (2014). Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers. Sensors, 14(11), 20519-20532. https://doi.org/10.3390/s141120519

© 2014 by the authors; licensee MDPI, Basel, Switzerland. This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of... Read More about Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers.