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Environmental niche variation and evolutionary diversification of the Brachypodium distachyon grass complex species in their native circum-mediterranean range (2015)
Journal Article
López-Alvarez, D., Manzaneda, A. J., Rey, P. J., Giraldo, P., Benavente, E., Allainguillaume, J., …Catalán, P. (2015). Environmental niche variation and evolutionary diversification of the Brachypodium distachyon grass complex species in their native circum-mediterranean range. American Journal of Botany, 102(7), 1073-1088. https://doi.org/10.3732/ajb.1500128

© 2015 Botanical Society of America. PREMISE OF THE STUDY: We conducted environmental niche modeling (ENM) of the Brachypodium distachyon s.l. complex, a model group of two diploid annual grasses (B. distachyon, B. stacei) and their derived allotetra... Read More about Environmental niche variation and evolutionary diversification of the Brachypodium distachyon grass complex species in their native circum-mediterranean range.

High-resolution melt and morphological analyses of mealybugs (Hemiptera: Pseudococcidae) from cacao: Tools for the control of Cacao swollen shoot virus spread (2015)
Journal Article
Wetten, A., Campbell, C., & Allainguillaume, J. (2016). High-resolution melt and morphological analyses of mealybugs (Hemiptera: Pseudococcidae) from cacao: Tools for the control of Cacao swollen shoot virus spread. Pest Management Science, 72(3), 527-533. https://doi.org/10.1002/ps.4017

© 2015 Society of Chemical Industry. BACKGROUND: Mealybugs (Hemiptera: Coccoidea: Pseudococcidae) are key vectors of badnaviruses, including Cacao swollen shoot virus (CSSV), the most damaging virus affecting cacao (Theobroma cacao L.). The effective... Read More about High-resolution melt and morphological analyses of mealybugs (Hemiptera: Pseudococcidae) from cacao: Tools for the control of Cacao swollen shoot virus spread.

Assessing the value of imperfect biocontainment nationally: Rapeseed in the United Kingdom as an exemplar (2014)
Journal Article
Ford, C. S., Allainguillaume, J., Fu, T. R., Mitchley, J., & Wilkinson, M. J. (2015). Assessing the value of imperfect biocontainment nationally: Rapeseed in the United Kingdom as an exemplar. New Phytologist, 205(3), 1342-1349. https://doi.org/10.1111/nph.13131

© 2014 New Phytologist Trust. Paternal biocontainment methods (PBMs) act by preventing pollen-mediated transgene flow. They are compromised by transgene escape via the crop-maternal line. We therefore assess the efficacy of PBMs for transgenic rapese... Read More about Assessing the value of imperfect biocontainment nationally: Rapeseed in the United Kingdom as an exemplar.

Investigation on Cacao swollen shoot virus (CSSV) pollen transmission through cross-pollination (2013)
Journal Article
Ameyaw, G. A., Wetten, A., Dzahini-Obiatey, H., Domfeh, O., & Allainguillaume, J. (2013). Investigation on Cacao swollen shoot virus (CSSV) pollen transmission through cross-pollination. Plant Pathology, 62(2), 421-427. https://doi.org/10.1111/j.1365-3059.2012.02640.x

DNA- and RNA-based polymerase chain reaction (PCR) systems were used with Cacao swollen shoot virus (CSSV) primers designed from conserved regions of the six published genomic sequences of CSSV to investigate whether the virus is transmissible from i... Read More about Investigation on Cacao swollen shoot virus (CSSV) pollen transmission through cross-pollination.

Analyzing sward and plant species make-up of sports and amenity turf, involves analyzing presence of genetic markers e.g. ribulose-bisphosphate carboxylase gene in sward sample obtained from sports or amenity turf surfaces (2012)
Patent
Allainguillaume, J., & Wilkinson, M. J. (2012). Analyzing sward and plant species make-up of sports and amenity turf, involves analyzing presence of genetic markers e.g. ribulose-bisphosphate carboxylase gene in sward sample obtained from sports or amenity turf surfaces. WO2012131300-A2 ; WO2012131300-A3

Abstract: NOVELTY - Method for analyzing sward, involves analyzing a sample of sward for the presence of one or more genetic markers, where the one or more genetic markers indicates the presence, identity and/or relative abundance of one or more spec... Read More about Analyzing sward and plant species make-up of sports and amenity turf, involves analyzing presence of genetic markers e.g. ribulose-bisphosphate carboxylase gene in sward sample obtained from sports or amenity turf surfaces.

DNA barcoding the native flowering plants and conifers of wales (2012)
Journal Article
Rich, T. C., Steinke, D., de Vere, N., Ford, C. R., Trinder, S. A., Long, C., …Wilkinson, M. J. (2012). DNA barcoding the native flowering plants and conifers of wales. PLoS ONE, 7(6), e37945. https://doi.org/10.1371/journal.pone.0037945

We present the first national DNA barcode resource that covers the native flowering plants and conifers for the nation of Wales (1143 species). Using the plant DNA barcode markers rbcL and matK, we have assembled 97.7% coverage for rbcL, 90.2% for ma... Read More about DNA barcoding the native flowering plants and conifers of wales.

Maintaining functional major histocompatibility complex diversity under inbreeding: The case of a selfing vertebrate (2012)
Journal Article
Allainguillaume, J., Ellison, A., Allainguillaume, J., Girdwood, S., Pachebat, J., Peat, K. M., …Consuegra, S. (2012). Maintaining functional major histocompatibility complex diversity under inbreeding: The case of a selfing vertebrate. Proceedings of the Royal Society B: Biological Sciences, 279(1749), 5004-5013. https://doi.org/10.1098/rspb.2012.1929

Major histocompatibility complex (MHC) genes encode proteins that present pathogen-derived antigens to T-cells, initiating the adaptive immune response in vertebrates. Although populations with low MHC diversity tend to be more susceptible to pathoge... Read More about Maintaining functional major histocompatibility complex diversity under inbreeding: The case of a selfing vertebrate.

Exploiting the brachypodium tool box in cereal and grass research (2011)
Journal Article
Mur, L. A., Allainguillaume, J., Catalán, P., Hasterok, R., Jenkins, G., Lesniewska, K., …Vogel, J. (2011). Exploiting the brachypodium tool box in cereal and grass research. New Phytologist, 191(2), 334-347. https://doi.org/10.1111/j.1469-8137.2011.03748.x

It is now a decade since Brachypodium distachyon (Brachypodium) was suggested as a model species for temperate grasses and cereals. Since then transformation protocols, large expressed sequence tag (EST) databases, tools for forward and reverse genet... Read More about Exploiting the brachypodium tool box in cereal and grass research.

Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2010 - 30 September 2010 (2011)
Journal Article
Smulders, M. J., Reusch, T. B., Pinheiro, J. B., Nguyen, T. T., Messmer, A. M., Goodisman, M. A., …Zucchi, M. I. (2011). Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2010 - 30 September 2010. Molecular Ecology Resources, 11(1), 219-222. https://doi.org/10.1111/j.1755-0998.2010.02944.x

This article documents the addition of 229 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Acacia auriculiformis-×-Acacia mangium hybrid, Alabama argillacea, Anoplopoma fimbria, A... Read More about Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2010 - 30 September 2010.

Use of airborne remote sensing to detect riverside Brassica rapa to aid in risk assessment of transgenic crops (2009)
Journal Article
Elliott, L. M., Mason, D. C., Allainguillaume, J., & Wilkinson, M. J. (2009). Use of airborne remote sensing to detect riverside Brassica rapa to aid in risk assessment of transgenic crops. Journal of Applied Remote Sensing, 3(1), 033562. https://doi.org/10.1117/1.3269615

High resolution descriptions of plant distribution have utility for many ecological applications but are especially useful for predictive modeling of gene flow from transgenic crops. Difficulty lies in the extrapolation errors that occur when limited... Read More about Use of airborne remote sensing to detect riverside Brassica rapa to aid in risk assessment of transgenic crops.

Rapeseed cytoplasm gives advantage in wild relatives and complicates genetically modified crop biocontainment (2009)
Journal Article
Allainguillaume, J., Harwood, T., Ford, C. S., Cuccato, G., Norris, C., Allender, C. J., …Wilkinson, M. J. (2009). Rapeseed cytoplasm gives advantage in wild relatives and complicates genetically modified crop biocontainment. New Phytologist, 183(4), 1201-1211. https://doi.org/10.1111/j.1469-8137.2009.02877.x

Biocontainment methods for genetically modified crops closest to commercial reality (chloroplast transformation, male sterility) would be compromised (in absolute terms) by seed-mediated gene flow leading to chloroplast capture. Even in these circums... Read More about Rapeseed cytoplasm gives advantage in wild relatives and complicates genetically modified crop biocontainment.

Spontaneous capture of oilseed rape (Brassica napus) chloroplasts by wild B. rapa: Implications for the use of chloroplast transformation for biocontainment (2009)
Journal Article
Haider, N., Allainguillaume, J., & Wilkinson, M. J. (2009). Spontaneous capture of oilseed rape (Brassica napus) chloroplasts by wild B. rapa: Implications for the use of chloroplast transformation for biocontainment. Current Genetics, 55(2), 139-150. https://doi.org/10.1007/s00294-009-0230-5

Environmental concerns over the cultivation of Genetically Modified (GM) crops largely centre on the ecological consequences following gene flow to wild relatives. One attractive solution is to deploy biocontainment measures that prevent hybridizatio... Read More about Spontaneous capture of oilseed rape (Brassica napus) chloroplasts by wild B. rapa: Implications for the use of chloroplast transformation for biocontainment.

Transmission of cocoa swollen shoot virus by seeds (2008)
Journal Article
Quainoo, A. K., Wetten, A. C., & Allainguillaume, J. (2008). Transmission of cocoa swollen shoot virus by seeds. Journal of Virological Methods, 150(1-2), 45-49. https://doi.org/10.1016/j.jviromet.2008.03.009

A study was undertaken to determine whether cocoa swollen shoot virus is transmitted by seeds, to improve the robustness of quarantine procedures for international exchange and long term conservation of cocoa germplasm. PCR/capillary electrophoresis,... Read More about Transmission of cocoa swollen shoot virus by seeds.

The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees (2008)
Journal Article
Quainoo, A. K., Wetten, A. C., & Allainguillaume, J. (2008). The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees. Journal of Virological Methods, 149(1), 91-96. https://doi.org/10.1016/j.jviromet.2008.01.007

Investigations were undertaken on the use of somatic embryogenesis to generate cocoa swollen shoot virus (CSSV) disease free clonal propagules from infected trees. Polymerase chain reaction (PCR) capillary electrophoresis revealed the presence of CSS... Read More about The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees.

Simple sequence repeats reveal uneven distribution of genetic diversity in chloroplast genomes of Brassica oleracea L. and (n = 9) wild relatives (2007)
Journal Article
Allender, C. J., Allainguillaume, J., Lynn, J., & King, G. J. (2007). Simple sequence repeats reveal uneven distribution of genetic diversity in chloroplast genomes of Brassica oleracea L. and (n = 9) wild relatives. TAG Theoretical and Applied Genetics, 114(4), 609-618. https://doi.org/10.1007/s00122-006-0461-5

Diversity in the chloroplast genome of 171 accessions representing the Brassica 'C' (n = 9) genome, including domesticated and wild B. oleracea and nine inter-fertile related wild species, was investigated using six chloroplast SSR (microsatellite) m... Read More about Simple sequence repeats reveal uneven distribution of genetic diversity in chloroplast genomes of Brassica oleracea L. and (n = 9) wild relatives.

Spontaneous gene flow from rapeseed (Brassica napus) to wild Brassica oleracea (2006)
Journal Article
Wilkinson, M. J., Allender, C. J., Ford, C. S., Allainguillaume, J., Grilli-Chantler, P., & Cuccato, G. (2006). Spontaneous gene flow from rapeseed (Brassica napus) to wild Brassica oleracea. Proceedings of the Royal Society B: Biological Sciences, 273(1605), 3111-3115. https://doi.org/10.1098/rspb.2006.3686

Research on the environmental risks of gene flow from genetically modified (GM) crops to wild relatives has traditionally emphasized recipients yielding most hybrids. For GM rapeseed (Brassica napus), interest has centred on the 'frequently hybridizi... Read More about Spontaneous gene flow from rapeseed (Brassica napus) to wild Brassica oleracea.

Fitness of hybrids between rapeseed (Brassica napus) and wild Brassica rapa in natural habitats (2006)
Journal Article
Allainguillaume, J., Alexander, M., Bullock, J. M., Saunders, M., Allender, C. J., King, G., …Wilkinson, M. J. (2006). Fitness of hybrids between rapeseed (Brassica napus) and wild Brassica rapa in natural habitats. Molecular Ecology, 15(4), 1175-1184. https://doi.org/10.1111/j.1365-294X.2006.02856.x

Fitness of hybrids between genetically modified (GM) crops and wild relatives influences the likelihood of ecological harm. We measured fitness components in spontaneous (non-GM) rapeseed x Brassica rapa hybrids in natural populations. The F1 hybrids... Read More about Fitness of hybrids between rapeseed (Brassica napus) and wild Brassica rapa in natural habitats.

Genetic diversity and structure of natural and managed populations of Cedrus atlantica (Pinaceae) assessed using random amplified polymorphic DNA (2005)
Journal Article
Renau-Morata, B., Nebauer, S. G., Sales, E., Allainguillaume, J., Caligari, P., & Segura, J. (2005). Genetic diversity and structure of natural and managed populations of Cedrus atlantica (Pinaceae) assessed using random amplified polymorphic DNA. American Journal of Botany, 92(5), 875-884. https://doi.org/10.3732/ajb.92.5.875

Cedrus atlantica (Pinaceae) is a large and exceptionally long-lived conifer native to the Rif and Atlas Mountains of North Africa. To assess levels and patterns of genetic diversity of this species, samples were obtained throughout the natural range... Read More about Genetic diversity and structure of natural and managed populations of Cedrus atlantica (Pinaceae) assessed using random amplified polymorphic DNA.

The role of satellite image-processing for national-scale estimates of gene flow from genetically modified crops: Rapeseed in the UK as a model (2004)
Journal Article
Elliott, L. J., Mason, D. C., Wilkinson, M. J., Allainguillaume, J., Norris, C., Alexander, M., & Welters, R. (2004). The role of satellite image-processing for national-scale estimates of gene flow from genetically modified crops: Rapeseed in the UK as a model. Journal of Applied Ecology, 41(6), 1174-1184. https://doi.org/10.1111/j.0021-8901.2004.00963.x

1. There is concern over the possibility of unwanted environmental change following transgene movement from genetically modified (GM) rapeseed Brassica napus to its wild and weedy relatives. 2. The aim of this research was to develop a remote sensing... Read More about The role of satellite image-processing for national-scale estimates of gene flow from genetically modified crops: Rapeseed in the UK as a model.