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Leukaemic and bone marrow stromal cells alter cytarabine genotoxicity and cytotoxicity in favour of disease (2019)
Journal Article
May, J., Gynn, L., Anderson, E., & Robinson, G. (2019). Leukaemic and bone marrow stromal cells alter cytarabine genotoxicity and cytotoxicity in favour of disease. Mutagenesis, 34(5/6), e5. https://doi.org/10.1093/mutage/gez025

Resistance to therapy remains one of the greatest challenges in the management of the blood and bone marrow (BM) disorder, acute myeloid leukaemia (AML). This study aimed to investigate how leukaemic and BM stromal cells contribute to altered chemo-s... Read More about Leukaemic and bone marrow stromal cells alter cytarabine genotoxicity and cytotoxicity in favour of disease.

Influence of Bone Marrow Stromal and Leukemic Cells on Cytarabine Chemo-Toxicity in Acute Myeloid Leukemia (AML). (2019)
Journal Article
Gynn, L. E., Anderson, E., Robinson, G. M., Wexler, S. A., Upstill-Goddard, G., Cox, C., …Morgan, R. G. (2019). Influence of Bone Marrow Stromal and Leukemic Cells on Cytarabine Chemo-Toxicity in Acute Myeloid Leukemia (AML). Blood, 134(Supplement 1), 2492. https://doi.org/10.1182/blood-2019-122891

Mesenchymal stromal cells (MSC) are known to protect leukemic cells from drug-induced toxicity within the bone marrow (BM) niche, however, less is known about leukemic impact on supportive MSC. The nucleoside-analogue, cytarabine (Ara-C), is a front-... Read More about Influence of Bone Marrow Stromal and Leukemic Cells on Cytarabine Chemo-Toxicity in Acute Myeloid Leukemia (AML)..

Chemotherapy-induced genotoxic damage to bone marrow cells: Long-term implications (2018)
Journal Article
May, J. E., Donaldson, C., Gynn, L., & Ruth Morse, H. (2018). Chemotherapy-induced genotoxic damage to bone marrow cells: Long-term implications. Mutagenesis, 33(3), 241-251. https://doi.org/10.1093/mutage/gey014

© The Author(s) 2018. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. Mesenchymal stem/stromal cells (MSCs) within the bone marrow (BM) are vitally important in forming the micro-environment supporting haematop... Read More about Chemotherapy-induced genotoxic damage to bone marrow cells: Long-term implications.

Rapid in vitro testing and characterization of resistance to chemotherapeutic agents daunorubicin and cytarabine in acute myeloid leukaemia (2017)
Journal Article
May, J., Steven, J., Taylor, A., Dean, S., & Anderson, E. (2017). Rapid in vitro testing and characterization of resistance to chemotherapeutic agents daunorubicin and cytarabine in acute myeloid leukaemia. Mutagenesis, 32(6), 627. https://doi.org/10.1093/mutage/gex033

Treatment success in leukaemia is impacted by patient resistance to chemotherapy including cytarabine (ara-C) and the anthracycline daunorubicin (DNR) requiring rapid pre-screening testing. DNR is converted to daunorubicinol (DNRol) by carbonyl reduc... Read More about Rapid in vitro testing and characterization of resistance to chemotherapeutic agents daunorubicin and cytarabine in acute myeloid leukaemia.

Development of a 2D leukaemia-stroma model for analyses of chemo-toxicity protection using a novel bacterial bioluminescent biosensor (2017)
Journal Article
Gynn, L., May, J., Anderson, E., & Robinson, G. (2017). Development of a 2D leukaemia-stroma model for analyses of chemo-toxicity protection using a novel bacterial bioluminescent biosensor. Mutagenesis, 32(6), 619. https://doi.org/10.1093/mutage/gex033

This study aimed to develop a 2D leukaemia-stroma model of the bone marrow for analysis of stromal-mediated chemo-protection; known to contribute to chemotherapy resistance. The model must facilitate integration of a bioluminescent biosensor HA1 for... Read More about Development of a 2D leukaemia-stroma model for analyses of chemo-toxicity protection using a novel bacterial bioluminescent biosensor.

Cytotoxicity and genotoxicity of electrochemically activated solutions (ECAS) (2017)
Journal Article
May, J., Robinson, G., Thorn, R., & Reynolds, D. (2017). Cytotoxicity and genotoxicity of electrochemically activated solutions (ECAS). Mutagenesis, 32(6), 622-623. https://doi.org/10.1093/mutage/gex033

Electrochemically activated solutions (ECAS) are of interest as potential disinfectants due to high biocidal activity against many microorganisms. They are widely used clinically in some countries (decontamination of surgical instruments and wounds),... Read More about Cytotoxicity and genotoxicity of electrochemically activated solutions (ECAS).

A novel biosensor for prediction of global hypomethylation by decitabine and azacytadine in leukaemic cells (2015)
Journal Article
May, J. E., Allainguillaume, J., Page, K., Stratford-Smith, C., Aruomaren, A., Alloush, H., …Anderson, E. (2015). A novel biosensor for prediction of global hypomethylation by decitabine and azacytadine in leukaemic cells. Mutagenesis, 30(6), 856. https://doi.org/10.1093/mutage/gev074

Decitabine (DAC) and azacytidine (AZA) are cytidine ana- logues and hypomethylating agents used in the manage- ment of patients with myelodysplastic syndrome (MDS), where the main goal is to prevent transformation to acute myeloid leukaemia (AML), wh... Read More about A novel biosensor for prediction of global hypomethylation by decitabine and azacytadine in leukaemic cells.

The stromal cell line HS-5 is an alternative mesenchymal stem cell source for the development of a 3D bone marrow model (2015)
Journal Article
Kabrah, S., May, J. E., Donaldson, C., & Morse, H. R. (2015). The stromal cell line HS-5 is an alternative mesenchymal stem cell source for the development of a 3D bone marrow model. Mutagenesis, 30(6), 861. https://doi.org/10.1093/mutage/gev074

Developing a novel three-dimensional (3D) in vitro model of the bone marrow microenvironment using bone mar- row mesenchymal stem cells (BM-MSCs) in commercial matrices involves a series of distinct processes. This starts with selecting an appropriat... Read More about The stromal cell line HS-5 is an alternative mesenchymal stem cell source for the development of a 3D bone marrow model.

Determination of the appropriate gene for real-time PCR analysis of the immotalized stromal cell line HS-5 in 2D and 3D culture following melphalan exposure (2015)
Journal Article
Kabrah, S., May, J. E., Donaldson, C., & Morse, H. R. (2015). Determination of the appropriate gene for real-time PCR analysis of the immotalized stromal cell line HS-5 in 2D and 3D culture following melphalan exposure. Mutagenesis, 30(6), 874. https://doi.org/10.1093/mutage/gev074

Although gene expression analysis using real-time quan- titative reverse transcription-polymerase chain reaction (RT-qPCR) is an accurate method to evaluate cell activi- ties and phenotypes, the reliability of the analysis depends on the selection of... Read More about Determination of the appropriate gene for real-time PCR analysis of the immotalized stromal cell line HS-5 in 2D and 3D culture following melphalan exposure.

Bone marrow and myeloma cellular communication protects from genotoxicity in a drug-specific manner (2015)
Journal Article
Andrews, S., Morse, H. R., & May, J. E. (2015). Bone marrow and myeloma cellular communication protects from genotoxicity in a drug-specific manner. Mutagenesis, 30(6), 867-868. https://doi.org/10.1093/mutage/gev074

Multiple myeloma (MM) is an incurable haematological malignancy with most patients eventually developing ad- vanced, relapsing disease and drug resistance (1,2). This may result from protection by BM mesenchymal cells (BM— MSC) interacting with MM ce... Read More about Bone marrow and myeloma cellular communication protects from genotoxicity in a drug-specific manner.

Effects of bone marrow stromal cells on sensitivity of leukaemic cells to chemotherapy (2013)
Journal Article
Page, K., Anderson, E., Smith, A., Reid, C., Salisbury, V., Donaldson, C., & May, J. E. (2014). Effects of bone marrow stromal cells on sensitivity of leukaemic cells to chemotherapy. Mutagenesis, 29(1), 92. https://doi.org/10.1093/mutage/get060

Cytosine arabinoside (ara-C) is a key chemotherapy drug used to treat acute myeloid leukaemia (AML), however, ap- proximately 30% of patients do not respond to this therapy. 92 An evaluative assay to determine resistance/sensitivity to ara- C prior... Read More about Effects of bone marrow stromal cells on sensitivity of leukaemic cells to chemotherapy.

Chemotherapy-induced cytotoxicity and genotoxicity to the bone marrow microenvironment (2013)
Journal Article
May, J. E., Morse, H. R., Page, K., Soh, M., Wexler, S., Cox, C., …Donaldson, C. (2014). Chemotherapy-induced cytotoxicity and genotoxicity to the bone marrow microenvironment. Mutagenesis, 29(1), 79. https://doi.org/10.1093/mutage/get060

The bone marrow (BM) microenvironment is largely com- prised of mesenchymal stromal cells (MSCs) and is vital in supporting haematopoiesis. Limited studies have indicated that the BM microenvironment is damaged by chemotherapy; however the extent and... Read More about Chemotherapy-induced cytotoxicity and genotoxicity to the bone marrow microenvironment.

Use of whole blood for analysis of disease-associated biomarkers (2013)
Journal Article
McLeod, J., Pemberton, R. M., May, J. E., Pemberton, R., Hart, J. P., McLeod, J. D., …Doran, O. (2013). Use of whole blood for analysis of disease-associated biomarkers. Analytical Biochemistry, 437(1), 59-61. https://doi.org/10.1016/j.ab.2013.02.024

Analyses for diagnosis and monitoring of pathological conditions often rely on blood samples, partly due to relative ease of collection. However, many interfering substances largely preclude the use of whole blood itself, necessitating separation of... Read More about Use of whole blood for analysis of disease-associated biomarkers.

Multiple myeloma: The bone marrow microenvironment and its relation to treatment (2013)
Journal Article
Andrews, S. W., Kabrah, S., May, J. E., Donaldson, C., & Morse, H. R. (2013). Multiple myeloma: The bone marrow microenvironment and its relation to treatment. British Journal of Biomedical Science, 70(3), 110-120. https://doi.org/10.1080/09674845.2013.11669945

Multiple myeloma is the most common haematological malignancy yet currently it remains incurable. For decades the mainstay in therapy has been non-targeted approaches including genotoxic agents and immunosuppressants. With myeloma predominantly affec... Read More about Multiple myeloma: The bone marrow microenvironment and its relation to treatment.

Development of a novel, physiologically relevant cytotoxicity model: Application to the study of chemotherapeutic damage to mesenchymal stromal cells (2012)
Journal Article
May, J. E., Morse, H. R., Xu, J., & Donaldson, C. (2012). Development of a novel, physiologically relevant cytotoxicity model: Application to the study of chemotherapeutic damage to mesenchymal stromal cells. Toxicology and Applied Pharmacology, 263(3), 374-389. https://doi.org/10.1016/j.taap.2012.07.013

There is an increasing need for development of physiologically relevant in-vitro models for testing toxicity, however determining toxic effects of agents which undergo extensive hepatic metabolism can be particularly challenging. If a source of such... Read More about Development of a novel, physiologically relevant cytotoxicity model: Application to the study of chemotherapeutic damage to mesenchymal stromal cells.


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