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μ-Opioid receptor desensitization: Homologous or heterologous? (2012)
Journal Article
Llorente, J., Lowe, J. D., Sanderson, H. S., Tsisanova, E., Kelly, E., Henderson, G., & Bailey, C. P. (2012). μ-Opioid receptor desensitization: Homologous or heterologous?. European Journal of Neuroscience, 36(12), 3636-3642. https://doi.org/10.1111/ejn.12003

There is considerable controversy over whether μ-opioid receptor (MOPr) desensitization is homologous or heterologous and over the mechanisms underlying such desensitization. In different cell types MOPr desensitization has been reported to involve r... Read More about μ-Opioid receptor desensitization: Homologous or heterologous?.

Phosphorylation of Mcl-1 by CDK1–cyclin B1 initiates its Cdc20-dependent destruction during mitotic arrest (2010)
Journal Article
Harley, M. E., Allan, L. A., Sanderson, H. S., & Clarke, P. R. (2010). Phosphorylation of Mcl-1 by CDK1–cyclin B1 initiates its Cdc20-dependent destruction during mitotic arrest. EMBO Journal, 29(14), 2407-2420. https://doi.org/10.1038/emboj.2010.112

The balance between cell cycle progression and apoptosis is important for both surveillance against genomic defects and responses to drugs that arrest the cell cycle. In this report, we show that the level of the human anti-apoptotic protein Mcl-1 is... Read More about Phosphorylation of Mcl-1 by CDK1–cyclin B1 initiates its Cdc20-dependent destruction during mitotic arrest.

A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM) (2007)
Journal Article
Allen, T. D., Rutherford, S. A., Murray, S., Sanderson, H., Gardiner, F., Kiseleva, E., …Drummond, S. P. (2007). A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM). Nature Protocols, 2(5), 1166-1172. https://doi.org/10.1038/nprot.2007.137

This protocol details methods for the isolation of oocyte nuclear envelopes (NEs) from the African clawed toad Xenopus laevis, immunogold labeling of component proteins and subsequent visualization by field-emission scanning electron microscopy (FESE... Read More about A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM).

Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro-formed nuclei for Western blotting and scanning electron microscopy (SEM) (2007)
Journal Article
Allen, T. D., Rutherford, S. A., Murray, S., Sanderson, H. S., Gardiner, F., Kiseleva, E., …Drummond, S. P. (2007). Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro-formed nuclei for Western blotting and scanning electron microscopy (SEM). Nature Protocols, 2(5), 1173-1179. https://doi.org/10.1038/nprot.2007.138

This protocol details methods for the generation of cell-free extracts and DNA templates from the eggs and sperm chromatin, respectively, of the clawed toad Xenopus laevis. We have used this system with scanning electron microscopy (SEM), as detailed... Read More about Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro-formed nuclei for Western blotting and scanning electron microscopy (SEM).

A mitotic role for BRCA1/BARD1 in tumor suppression? (2006)
Journal Article
Clarke, P. R., & Sanderson, H. S. (2006). A mitotic role for BRCA1/BARD1 in tumor suppression?. Cell, 127(3), 453-455. https://doi.org/10.1016/j.cell.2006.10.020

The tumor-suppressor protein BRCA1 is thought to act by preserving genomic integrity. In this issue of Cell, Joukov et al. (2006) demonstrate that the BRCA1/BARD1 heterodimer participates in mitotic spindle assembly, a process conducted by the GTPase... Read More about A mitotic role for BRCA1/BARD1 in tumor suppression?.

Cell biology: Ran, mitosis and the cancer connection (2006)
Journal Article
Sanderson, H. S., & Clarke, P. R. (2006). Cell biology: Ran, mitosis and the cancer connection. Current Biology, 16(12), PR466-R468. https://doi.org/10.1016/j.cub.2006.05.032

The small GTPase Ran has been shown to regulate HURP, a protein that interacts with several mitotic spindle assembly factors. This discovery sheds new light on the role of Ran in the fidelity of mitosis and in cancer. © 2006 Elsevier Ltd. All rights... Read More about Cell biology: Ran, mitosis and the cancer connection.

Nup214-Nup88 nucleoporin subcomplex is required for CRM1-mediated 60 S preribosomal nuclear export (2006)
Journal Article
Fornerod, M., Bernad, R., Engelsma, D., Sanderson, H., Pickersgill, H., & Fornerod, . M. (2006). Nup214-Nup88 nucleoporin subcomplex is required for CRM1-mediated 60 S preribosomal nuclear export. Journal of Biological Chemistry, 281(28), 19378-19386. https://doi.org/10.1074/jbc.M512585200

The nuclear pore complex (NPC) conducts macromolecular transport to and from the nucleus and provides a kinetic/hydrophobic barrier composed of phenylalanine-glycine (FG) repeats. Nuclear transport is achieved through permeation of this barrier by tr... Read More about Nup214-Nup88 nucleoporin subcomplex is required for CRM1-mediated 60 S preribosomal nuclear export.

High resolution analysis of mammalian nuclear structure throughout the cell cycle: Implications for nuclear pore complex assembly during interphase and mitosis (2006)
Journal Article
Drummond, S. P., Rutherford, S. A., Sanderson, H. S., & Allen, T. D. (2006). High resolution analysis of mammalian nuclear structure throughout the cell cycle: Implications for nuclear pore complex assembly during interphase and mitosis. Canadian Journal of Physiology and Pharmacology, 84(3-4), 423-430. https://doi.org/10.1139/y05-148

Nuclear pore complexes (NPCs) are the gateways for both active and passive bidirectional molecular transport between the nucleoplasm and cytoplasm. These mega-dalton assemblies are composed of multiple copies of approximately 30 distinct proteins ter... Read More about High resolution analysis of mammalian nuclear structure throughout the cell cycle: Implications for nuclear pore complex assembly during interphase and mitosis.