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Leukaemic and Bone Marrow Stromal Cells Alter Cytarabine Genotoxicity and Cytotoxicity in Favour of Disease (2019)
Journal Article
May, J., Gynn, L., Anderson, E., & Robinson, G. (2019). Leukaemic and Bone Marrow Stromal Cells Alter Cytarabine Genotoxicity and Cytotoxicity in Favour of Disease. Mutagenesis, 34(5/6), e5. https://doi.org/10.1093/mutage/gez025

Resistance to therapy remains one of the greatest challenges in the management of the blood and bone marrow (BM) disorder, acute myeloid leukaemia (AML). This study aimed to investigate how leukaemic and BM stromal cells contribute to altered chemo-s... Read More about Leukaemic and Bone Marrow Stromal Cells Alter Cytarabine Genotoxicity and Cytotoxicity in Favour of Disease.

Influence of Bone Marrow Stromal and Leukemic Cells on Cytarabine Chemo-Toxicity in Acute Myeloid Leukemia (AML). (2019)
Journal Article
Gynn, L. E., Anderson, E., Robinson, G. M., Wexler, S. A., Upstill-Goddard, G., Cox, C., …Morgan, R. G. (2019). Influence of Bone Marrow Stromal and Leukemic Cells on Cytarabine Chemo-Toxicity in Acute Myeloid Leukemia (AML). Blood, 134(Supplement 1), 2492. https://doi.org/10.1182/blood-2019-122891

Mesenchymal stromal cells (MSC) are known to protect leukemic cells from drug-induced toxicity within the bone marrow (BM) niche, however, less is known about leukemic impact on supportive MSC. The nucleoside-analogue, cytarabine (Ara-C), is a front-... Read More about Influence of Bone Marrow Stromal and Leukemic Cells on Cytarabine Chemo-Toxicity in Acute Myeloid Leukemia (AML)..

CPX-351 exhibits hENT-independent uptake and can be potentiated by fludarabine in leukaemic cells lines and primary refractory AML (2018)
Journal Article
Anderson, E., Mehta, P., Heywood, J., Rees, B., Bone, H., Robinson, G., …Mayer, L. (2018). CPX-351 exhibits hENT-independent uptake and can be potentiated by fludarabine in leukaemic cells lines and primary refractory AML. Leukemia Research, 74, 121-129. https://doi.org/10.1016/j.leukres.2018.08.007

© 2018 Elsevier Ltd CPX-351, a liposomal formulation co-encapsulating cytarabine and daunorubicin (DNR) in a synergistic 5:1 M ratio, has shown favourable response in newly diagnosed elderly high-risk AML. This study assessed intracellular ara-CTP le... Read More about CPX-351 exhibits hENT-independent uptake and can be potentiated by fludarabine in leukaemic cells lines and primary refractory AML.

Cytotoxicity and genotoxicity of electrochemically activated solutions (ECAS) (2017)
Journal Article
May, J., Robinson, G., Thorn, R., & Reynolds, D. (2017). Cytotoxicity and genotoxicity of electrochemically activated solutions (ECAS). Mutagenesis, 32(6), 622-623. https://doi.org/10.1093/mutage/gex033

Electrochemically activated solutions (ECAS) are of interest as potential disinfectants due to high biocidal activity against many microorganisms. They are widely used clinically in some countries (decontamination of surgical instruments and wounds),... Read More about Cytotoxicity and genotoxicity of electrochemically activated solutions (ECAS).

Development of a 2D leukaemia-stroma model for analyses of chemo-toxicity protection using a novel bacterial bioluminescent biosensor (2017)
Journal Article
Gynn, L., May, J., Anderson, E., & Robinson, G. (2017). Development of a 2D leukaemia-stroma model for analyses of chemo-toxicity protection using a novel bacterial bioluminescent biosensor. Mutagenesis, 32(6), 619. https://doi.org/10.1093/mutage/gex033

This study aimed to develop a 2D leukaemia-stroma model of the bone marrow for analysis of stromal-mediated chemo-protection; known to contribute to chemotherapy resistance. The model must facilitate integration of a bioluminescent biosensor HA1 for... Read More about Development of a 2D leukaemia-stroma model for analyses of chemo-toxicity protection using a novel bacterial bioluminescent biosensor.

Dual species biofilm culture of Staphylococcus aureus and a bioluminescent strain of Pseudomonas aeruginosa (2016)
Presentation / Conference
Steven, J., Robinson, G., & Nelson, S. (2016, October). Dual species biofilm culture of Staphylococcus aureus and a bioluminescent strain of Pseudomonas aeruginosa

Poster presentation. Introduction Pseudomonas aeruginosa and Staphylococcus aureus are significant pathogens of wounds and the cystic fibrosis lung. There are likely to be complex interactions between the two species, with biofilm co-culture aff... Read More about Dual species biofilm culture of Staphylococcus aureus and a bioluminescent strain of Pseudomonas aeruginosa.

Low light optical detection of whole-cell bioluminescent bacterial bioreporters for visualisation of surface decontamination using nonthermal plasma (2013)
Presentation / Conference
Robinson, G. M., Thorn, R., Hancock, C., & Reynolds, D. M. (2013, May). Low light optical detection of whole-cell bioluminescent bacterial bioreporters for visualisation of surface decontamination using nonthermal plasma. Paper presented at 3rd International Conference on Bio-Sensing Technology

Introduction: Maintenance of a clean, uncontaminated healthcare environment is instrumental in the delivery of modern healthcare. Nonthermal plasma is a technology with potential applications for sterilisation and disinfection in biological and medi... Read More about Low light optical detection of whole-cell bioluminescent bacterial bioreporters for visualisation of surface decontamination using nonthermal plasma.

Comparative antimicrobial activities of aerosolized sodium hypochlorite, chlorine dioxide, and electrochemically activated solutions evaluated using a novel standardized assay (2013)
Journal Article
Reynolds, D. M., Thorn, R. M., Thorn, R., Reynolds, D. M., & Robinson, G. M. (2013). Comparative antimicrobial activities of aerosolized sodium hypochlorite, chlorine dioxide, and electrochemically activated solutions evaluated using a novel standardized assay. Antimicrobial Agents and Chemotherapy, 57(5), 2216-2225. https://doi.org/10.1128/AAC.02589-12

The main aim of this study was to develop a standardized experimental assay to enable differential antimicrobial comparisons of test biocidal aerosols. This study represents the first chlorine-matched comparative assessment of the antimicrobial activ... Read More about Comparative antimicrobial activities of aerosolized sodium hypochlorite, chlorine dioxide, and electrochemically activated solutions evaluated using a novel standardized assay.

Detection of intracellular ara-C/ara-CTP following treatment with the new anti-leukemic drug elacytarabine using a bioluminescent bacterial biosensor (2013)
Presentation / Conference
Robinson, G. M., Anderson, E., Salisbury, V., Mehta, P., Sandvold, M., & Reynolds, D. M. (2013, April). Detection of intracellular ara-C/ara-CTP following treatment with the new anti-leukemic drug elacytarabine using a bioluminescent bacterial biosensor. Poster presented at American Association for Cancer Research Annual Meeting

Cytarabine (ara-C) is an analogue of deoxycytidine with an established role in treating AML. Cytarabine depends on the human equilibrative nucleoside transporter-1 (hENT-1) for cellular entry (1). Loss of hENT-1 function in vitro has been shown to re... Read More about Detection of intracellular ara-C/ara-CTP following treatment with the new anti-leukemic drug elacytarabine using a bioluminescent bacterial biosensor.

The effect of long-term storage on the physiochemical and bactericidal properties of electrochemically activated solutions (2013)
Journal Article
Robinson, G., Thorn, R., & Reynolds, D. (2013). The effect of long-term storage on the physiochemical and bactericidal properties of electrochemically activated solutions. International Journal of Molecular Sciences, 14(1), 457-469. https://doi.org/10.3390/ijms14010457

Electrochemically activated solutions (ECAS) are generated by electrolysis of NaCl solutions, and demonstrate broad spectrum antimicrobial activity and high environmental compatibility. The biocidal efficacy of ECAS at the point of production is wide... Read More about The effect of long-term storage on the physiochemical and bactericidal properties of electrochemically activated solutions.

Electrochemically activated solutions: Evidence for antimicrobial efficacy and applications in healthcare environments (2012)
Journal Article
Reynolds, D. M., Greenman, J., Robinson, G. M., Lee, S. W., Thorn, R. M., Thorn, R., …Reynolds, D. M. (2012). Electrochemically activated solutions: Evidence for antimicrobial efficacy and applications in healthcare environments. European Journal of Clinical Microbiology and Infectious Diseases, 31(5), 641-653. https://doi.org/10.1007/s10096-011-1369-9

Due to the limitations associated with the use of existing biocidal agents, there is a need to explore new methods of disinfection to help maintain effective bioburden control, especially within the healthcare environment. The transformation of low m... Read More about Electrochemically activated solutions: Evidence for antimicrobial efficacy and applications in healthcare environments.

Application of bacterial bioluminescence to assess the efficacy of fast-acting biocides (2011)
Journal Article
Thorn, R. M., Robinson, G. M., Robinson, G., Tonks, K. M., Thorn, R., & Reynolds, D. M. (2011). Application of bacterial bioluminescence to assess the efficacy of fast-acting biocides. Antimicrobial Agents and Chemotherapy, 55(11), 5214-5219. https://doi.org/10.1128/AAC.00489-11

Traditional microbiological techniques are used to provide reliable data on the rate and extent of kill for a range of biocides. However, such techniques provide very limited data regarding the initial rate of kill of fast-acting biocides over very s... Read More about Application of bacterial bioluminescence to assess the efficacy of fast-acting biocides.

Evaluation of the efficacy of electrochemically activated solutions against nosocomial pathogens and bacterial endospores (2010)
Journal Article
Reynolds, D. M., Salisbury, V. C., Greenman, J., Robinson, G. M., Robinson, G. M., Lee, S. W., …Reynolds, D. M. (2010). Evaluation of the efficacy of electrochemically activated solutions against nosocomial pathogens and bacterial endospores. Letters in Applied Microbiology, 50(3), 289-294. https://doi.org/10.1111/j.1472-765X.2009.02790.x

Aims: Electrochemically activated solutions (ECAS) are generated from halide salt solutions via specially designed electrolytic cells. The active solutions are known to possess high biocidal activity against a wide range of target microbial species,... Read More about Evaluation of the efficacy of electrochemically activated solutions against nosocomial pathogens and bacterial endospores.