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Pseudomonas daroniae sp. nov. and Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK (2019)
Journal Article
Bueno-Gonzalez, V., Brady, C., Denman, S., Plummer, S., Allainguillaume, J., & Arnold, D. (2019). Pseudomonas daroniae sp. nov. and Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK. International Journal of Systematic and Evolutionary Microbiology, 69(11), 3368-3376. https://doi.org/10.1099/ijsem.0.003615

Twenty-two cream-coloured bacterial strains were isolated from oak trees affected by acute oak decline (AOD) in Southern England. Isolates were Gram-negative, motile, slightly curved rods, aerobic, non-spore-forming, catalase positive and oxidase pos... Read More about Pseudomonas daroniae sp. nov. and Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK.

Pseudomonas syringae: Enterprising epiphyte and stealthy parasite (2019)
Journal Article
Arnold, D., & Preston, G. (2019). Pseudomonas syringae: Enterprising epiphyte and stealthy parasite. Microbiology, 165(3), 251-253. https://doi.org/10.1099/mic.0.000715

© 2019 The Authors. Pseudomonas syringae is best known as a plant pathogenic bacterium that causes diseases in a multitude of hosts, and it has been used as a model organism to understand the biology of plant disease. Pathogenic and non-pathogenic is... Read More about Pseudomonas syringae: Enterprising epiphyte and stealthy parasite.

Supercoiling of an excised genomic island represses effector gene expression to prevent activation of host resistance (2018)
Journal Article
Neale, H., Jackson, R., Preston, G., & Arnold, D. (2018). Supercoiling of an excised genomic island represses effector gene expression to prevent activation of host resistance. Molecular Microbiology, 110(3), 444-454. https://doi.org/10.1111/mmi.14111

© 2018 The Authors Molecular Microbiology Published by John Wiley & Sons Ltd. The plant pathogen Pseudomonas syringae pv. phaseolicola, which causes halo blight disease of beans, contains a 106kb genomic island PPHGI-1. PPHGI-1 carries a gene, avrP... Read More about Supercoiling of an excised genomic island represses effector gene expression to prevent activation of host resistance.

Suppression of the in vitro growth and development of Microdochium nivale by phosphite (2018)
Journal Article
Dempsey, J., Wilson, I. D., Spencer-Phillips, P., & Arnold, D. L. (2018). Suppression of the in vitro growth and development of Microdochium nivale by phosphite. Plant Pathology, 67(6), 1296-1306. https://doi.org/10.1111/ppa.12846

© 2018 British Society for Plant Pathology The ascomycete fungus Microdochium nivale is a major pathogen of many species of the gramineae. Control measures rely heavily on chemical fungicides, making alternative means of disease reduction desirable.... Read More about Suppression of the in vitro growth and development of Microdochium nivale by phosphite.

Coping with environmental eukaryotes; identification of pseudomonas syringae genes during the interaction with alternative hosts or predators (2018)
Journal Article
Arnold, D. L., Dorati, F., Barrett, G. A., Sanchez-Contreras, M., Arseneault, T., San Jose, M., …Jackson, R. W. (2018). Coping with environmental eukaryotes; identification of pseudomonas syringae genes during the interaction with alternative hosts or predators. Microorganisms, 6(2), 32. https://doi.org/10.3390/microorganisms6020032

Understanding the molecular mechanisms underpinning the ecological success of plant pathogens is critical to develop strategies for controlling diseases and protecting crops. Recent observations have shown that plant pathogenic bacteria, particularly... Read More about Coping with environmental eukaryotes; identification of pseudomonas syringae genes during the interaction with alternative hosts or predators.

Taxonomy and identification of bacteria associated with acute oak decline (2017)
Journal Article
Brady, C., Arnold, D. L., McDonald, J., & Denman, S. (2017). Taxonomy and identification of bacteria associated with acute oak decline. World Journal of Microbiology and Biotechnology, 33(7), https://doi.org/10.1007/s11274-017-2296-4

© 2017, The Author(s). Acute oak decline (AOD) is a relatively newly described disorder affecting native oak species in Britain. Symptomatic trees are characterised by stem bleeds from vertical fissures, necrotic lesions in the live tissue beneath an... Read More about Taxonomy and identification of bacteria associated with acute oak decline.

Hydrogen sulfide causes excision of a genomic island in Pseudomonas syringae pv. phaseolicola (2017)
Journal Article
Neale, H., Deshappriya, N., Arnold, D. L., Wood, M. E., Whiteman, M., & Hancock, J. T. (2017). Hydrogen sulfide causes excision of a genomic island in Pseudomonas syringae pv. phaseolicola. European Journal of Plant Pathology, 149(4), 911-921. https://doi.org/10.1007/s10658-017-1240-7

© 2017, The Author(s). Hydrogen sulfide (H2S) is known to be an important signalling molecule in both animals and plants, despite its toxic nature. In plants it has been seen to control stomatal apertures, so altering the ability of bacteria to invad... Read More about Hydrogen sulfide causes excision of a genomic island in Pseudomonas syringae pv. phaseolicola.

Confocal microscopy reveals in planta dynamic interactions between pathogenic, avirulent and non-pathogenic Pseudomonas syringae strains (2017)
Journal Article
Beuzón, C. R., Arnold, D. L., Ruiz-Albert, J., Mansfield, J. W., Corry, D. S., Rufián, J. S., …Arnold, D. L. (2018). Confocal microscopy reveals in planta dynamic interactions between pathogenic, avirulent and non-pathogenic Pseudomonas syringae strains. Molecular Plant Pathology, 19(3), 537-551. https://doi.org/10.1111/mpp.12539

© 2017 BSPP AND JOHN WILEY & SONS LTD Recent advances in genomics and single-cell analysis have demonstrated the extraordinary complexity reached by microbial populations within their hosts. Communities range from complex multispecies groups to hom... Read More about Confocal microscopy reveals in planta dynamic interactions between pathogenic, avirulent and non-pathogenic Pseudomonas syringae strains.

Multi-locus sequence typing of Escherichia coli isolates with acquired ampC genes and ampC promoter mutations (2016)
Journal Article
Lawrance, L. M., Moore, P. C., Lewis, J. A., Moore, P. C. L., Arnold, D. L., & Lawrance, L. (2016). Multi-locus sequence typing of Escherichia coli isolates with acquired ampC genes and ampC promoter mutations. Diagnostic Microbiology and Infectious Disease, 86(3), 265-267. https://doi.org/10.1016/j.diagmicrobio.2016.08.008

© 2016 Elsevier Inc. Multi-locus sequence typing was used to reveal a high degree of diversity amongst the E. coli isolates with AmpC plasmid genes, and a high prevalence of the −32 mutation present.

A cross disciplinary embodiment: Exploring the impacts of embedding science communication principles in a collaborative learning space (2016)
Book Chapter
Weitkamp, E., & Arnold, D. L. (2016). A cross disciplinary embodiment: Exploring the impacts of embedding science communication principles in a collaborative learning space. In M. de Vries, & M. van der Sanden (Eds.), Science and Technology Education and Communication: Seeking Synergy, (63-84). Rotterdam: Sense Publishers

This chapter reports on the short term impacts of a workshop involving pupils and suggests ways in which by working together scientists, science communicators and teachers can co-develop engaging and inspirational learning opportunities that place sc... Read More about A cross disciplinary embodiment: Exploring the impacts of embedding science communication principles in a collaborative learning space.

A low frequency persistent reservoir of a genomic island in a pathogen population ensures island survival and improves pathogen fitness in a susceptible host (2016)
Journal Article
Neale, H., Laister, R., Payne, J., Preston, G., Jackson, R., & Arnold, D. L. (2016). A low frequency persistent reservoir of a genomic island in a pathogen population ensures island survival and improves pathogen fitness in a susceptible host. Environmental Microbiology, 18(11), 4144-4152. https://doi.org/10.1111/1462-2920.13482

The co-evolution of bacterial plant pathogens and their hosts is a complex and dynamic process. Host resistance imposes stress on invading pathogens that can lead to changes in the bacterial genome enabling the pathogen to escape host resistance. We... Read More about A low frequency persistent reservoir of a genomic island in a pathogen population ensures island survival and improves pathogen fitness in a susceptible host.

Pseudomonas syringae Differentiates into Phenotypically Distinct Subpopulations During Colonization of a Plant Host (2016)
Journal Article
Beuzón, C. R., Casadesús, J., López-Márquez, D., Sánchez-Romero, M. A., Rufián, J. S., Rufian, J. S., …Beuzon, C. R. (2016). Pseudomonas syringae Differentiates into Phenotypically Distinct Subpopulations During Colonization of a Plant Host. Environmental Microbiology, 18(10), 3593-3605. https://doi.org/10.1111/1462-2920.13497

© 2016 Society for Applied Microbiology and John Wiley & Sons Ltd Bacterial microcolonies with heterogeneous sizes are formed during colonization of Phaseolus vulgaris by Pseudomonas syringae. Heterogeneous expression of structural and regulatory c... Read More about Pseudomonas syringae Differentiates into Phenotypically Distinct Subpopulations During Colonization of a Plant Host.

Early changes in apoplast composition associated with defence and disease in interactions between Phaseolus vulgaris and the halo blight pathogen Pseudomonas syringae Pv. phaseolicola (2016)
Journal Article
Preston, G. M., Jackson, R. W., Neale, H. C., O'Leary, B. M., O'Leary, B., Neale, H., …Preston, G. (2016). Early changes in apoplast composition associated with defence and disease in interactions between Phaseolus vulgaris and the halo blight pathogen Pseudomonas syringae Pv. phaseolicola. Plant, Cell and Environment, 39(10), 2172-2184. https://doi.org/10.1111/pce.12770

© 2016 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd. The apoplast is the arena in which endophytic pathogens such as Pseudomonas syringae grow and interact with plant cells. Using metabolomic and ion analysis techniqu... Read More about Early changes in apoplast composition associated with defence and disease in interactions between Phaseolus vulgaris and the halo blight pathogen Pseudomonas syringae Pv. phaseolicola.

Rapid identification of bacteria associated with Acute Oak Decline by high resolution melt (HRM) analysis (2016)
Journal Article
Brady, C., Allainguillaume, J., Denman, S., & Arnold, D. L. (2016). Rapid identification of bacteria associated with Acute Oak Decline by high resolution melt (HRM) analysis. Letters in Applied Microbiology, 63(2), 89-95. https://doi.org/10.1111/lam.12593

Two Gram-negative Enterobacteriaceae, Gibbsiella quercinecans and Brenneria goodwinii, are frequently isolated from oak suffering from Acute Oak Decline. These two species are difficult to identify based on colony morphology, carbohydrate utilisation... Read More about Rapid identification of bacteria associated with Acute Oak Decline by high resolution melt (HRM) analysis.

Gibbsiella papilionis Kim et al. 2013 is a later heterotypic synonym of gibbsiella dentisursi Saito et al. 2013 (2015)
Journal Article
Arnold, D., Brady, C., Irisawa, T., Iino, T., Ohkuma, M., Arnold, D. L., & Denman, S. (2015). Gibbsiella papilionis Kim et al. 2013 is a later heterotypic synonym of gibbsiella dentisursi Saito et al. 2013. International Journal of Systematic and Evolutionary Microbiology, 65(12), 4788-4791. https://doi.org/10.1099/ijsem.0.000649

© 2015 IUMS. Synonymy of Gibbsiella dentisursi DSM 23818T (=NUM 1720T) and Gibbsiella papilionis JCM 18389T (=LEN33T) was suspected following multilocus sequence analysis (MLSA) of both type strains in a previous classification study, where they were... Read More about Gibbsiella papilionis Kim et al. 2013 is a later heterotypic synonym of gibbsiella dentisursi Saito et al. 2013.

The identification of genes important in pseudomonas syringae pv. phaseolicola plant colonisation using in vitro screening of transposon libraries (2015)
Journal Article
Jackson, R. W., Neale, H. C., Manoharan, B., Neale, H., Hancock, J. T., Jackson, R., & Arnold, D. L. (2015). The identification of genes important in pseudomonas syringae pv. phaseolicola plant colonisation using in vitro screening of transposon libraries. PLoS ONE, 10(9), https://doi.org/10.1371/journal.pone.0137355

© 2015 Manoharan et al. The bacterial plant pathogen Pseudomonas syringae pv. phaseolicola (Pph) colonises the surface of common bean plants before moving into the interior of plant tissue, via wounds and stomata. In the intercellular spaces the path... Read More about The identification of genes important in pseudomonas syringae pv. phaseolicola plant colonisation using in vitro screening of transposon libraries.

Chromosomal ampC mutations in cefpodoxime-resistant ESBL-negative uropathogenic escherichia coli (2015)
Journal Article
Lewis, J. A., Moore, P. M., Arnold, D. L., & Lawrance, L. (2015). Chromosomal ampC mutations in cefpodoxime-resistant ESBL-negative uropathogenic escherichia coli. British Journal of Biomedical Science, 72(1), 7-11. https://doi.org/10.1080/09674845.2015.11666789

AmpC beta-lactamase is an enzyme commonly produced by Escherichia coli that causes resistance to cephalosporins and penicillins. Enzyme production is controlled by the strength of the promoter encoded by the chromosomal ampC gene, with the level of p... Read More about Chromosomal ampC mutations in cefpodoxime-resistant ESBL-negative uropathogenic escherichia coli.

Draft genome sequence of Pseudomonas corrugata, a phytopathogenic bacterium with potential industrial applications (2014)
Journal Article
Arnold, D. L., Licciardello, G., Jackson, R. W., Bella, P., Strano, C. P., Catara, A. F., …Catara, V. (2014). Draft genome sequence of Pseudomonas corrugata, a phytopathogenic bacterium with potential industrial applications. Journal of Biotechnology, 175(1), 65-66. https://doi.org/10.1016/j.jbiotec.2014.02.003

Pseudomonas corrugata was first described as the causal agent of a tomato disease called 'pith necrosis' yet it is considered as a biological resource in various fields such as biocontrol of plant diseases and production of industrially promising mic... Read More about Draft genome sequence of Pseudomonas corrugata, a phytopathogenic bacterium with potential industrial applications.

Rahnella victoriana sp. nov., Rahnella bruchi sp. nov., Rahnella woolbedingensis sp. nov., classification of Rahnella genomospecies 2 and 3 as Rahnella variigena sp. nov. and Rahnella inusitata sp. nov., respectively and emended description of the genus R (2014)
Journal Article
Arnold, D., Brady, C., Hunter, G., Kirk, S., Arnold, D. L., & Denman, S. (2014). Rahnella victoriana sp. nov., Rahnella bruchi sp. nov., Rahnella woolbedingensis sp. nov., classification of Rahnella genomospecies 2 and 3 as Rahnella variigena sp. nov. and Rahnella inusitata sp. nov., respectively and emended description of the genus Rahnella. Systematic and Applied Microbiology, 37(8), 545-552. https://doi.org/10.1016/j.syapm.2014.09.001

© 2014 Published by Elsevier GmbH. Isolations from oak symptomatic of Acute Oak Decline, alder and walnut log tissue, and buprestid beetles in 2009-2012 yielded 32 Gram-negative bacterial strains showing highest gyrB sequence similarity to Rahnella a... Read More about Rahnella victoriana sp. nov., Rahnella bruchi sp. nov., Rahnella woolbedingensis sp. nov., classification of Rahnella genomospecies 2 and 3 as Rahnella variigena sp. nov. and Rahnella inusitata sp. nov., respectively and emended description of the genus R.

The rulB gene of plasmid pWW0 is a hotspot for the site-specific insertion of integron-like elements found in the chromosomes of environmental Pseudomonas fluorescens group bacteria (2014)
Journal Article
Pickup, R. W., Jackson, R. W., Rhodes, G., Bosma, H., Studholme, D., Arnold, D. L., …Pickup, R. (2014). The rulB gene of plasmid pWW0 is a hotspot for the site-specific insertion of integron-like elements found in the chromosomes of environmental Pseudomonas fluorescens group bacteria. Environmental Microbiology, 16(8), 2374-2388. https://doi.org/10.1111/1462-2920.12345

Summary: The rulAB operon of Pseudomonas spp. confers fitness traits on the host and has been suggested to be a hotspot for insertion of mobile elements that carry avirulence genes. Here, for the first time, we show that rulB on plasmid pWW0 is a hot... Read More about The rulB gene of plasmid pWW0 is a hotspot for the site-specific insertion of integron-like elements found in the chromosomes of environmental Pseudomonas fluorescens group bacteria.