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Evaluation of the properties of a superficially porous silica stationary phase in hydrophilic interaction chromatography (2008)
Journal Article
McCalley, D. V. (2008). Evaluation of the properties of a superficially porous silica stationary phase in hydrophilic interaction chromatography. Journal of Chromatography A, 1193(1-2), 85-91. https://doi.org/10.1016/j.chroma.2008.04.007

The sample capacity, column efficiency (and its variation with flow) of a superficially porous unbonded silica phase (Halo) was investigated using hydrophilic interaction chromatography (HILIC), particularly for separation of basic compounds. Sample... Read More about Evaluation of the properties of a superficially porous silica stationary phase in hydrophilic interaction chromatography.

Estimation of the extent of the water-rich layer associated with the silica surface in hydrophilic interaction chromatography (2008)
Journal Article
McCalley, D. V., & Neue, U. D. (2008). Estimation of the extent of the water-rich layer associated with the silica surface in hydrophilic interaction chromatography. Journal of Chromatography A, 1192(2), 225-229. https://doi.org/10.1016/j.chroma.2008.03.049

The possible presence of a mobile phase layer rich in water on the surface of silica columns used under conditions typical in hydrophilic interaction chromatography was investigated by the injection of a small hydrophobic solute (benzene) using aceto... Read More about Estimation of the extent of the water-rich layer associated with the silica surface in hydrophilic interaction chromatography.

Analysis of basic compounds by reversed-phase high-performance liquid chromatography using hybrid inorganic/organic phases at high pH (2008)
Journal Article
Davies, N. H., Euerby, M. R., & McCalley, D. V. (2008). Analysis of basic compounds by reversed-phase high-performance liquid chromatography using hybrid inorganic/organic phases at high pH. Journal of Chromatography A, 1178(1-2), 71-78. https://doi.org/10.1016/j.chroma.2007.11.040

The retention and mass overload of five bases and a quaternary ammonium compound were studied on a bridged ethyl hybrid inorganic/organic phase (XBridge C18) over a pH range 2.7-12.0 using acetonitrile-phosphate and carbonate buffers. Some comparison... Read More about Analysis of basic compounds by reversed-phase high-performance liquid chromatography using hybrid inorganic/organic phases at high pH.

Is hydrophilic interaction chromatography with silica columns a viable alternative to reversed-phase liquid chromatography for the analysis of ionisable compounds? (2007)
Journal Article
McCalley, D. V. (2007). Is hydrophilic interaction chromatography with silica columns a viable alternative to reversed-phase liquid chromatography for the analysis of ionisable compounds?. Journal of Chromatography A, 1171(1-2), 46-55. https://doi.org/10.1016/j.chroma.2007.09.047

The separation of acidic, neutral and particularly basic solutes was investigated using a bare silica column, mostly under hydrophilic interaction chromatography (HILIC) conditions with water concentrations >2.5% and with >70% acetonitrile (ACN). Pro... Read More about Is hydrophilic interaction chromatography with silica columns a viable alternative to reversed-phase liquid chromatography for the analysis of ionisable compounds?.

A study of retention and overloading of basic compounds with mixed-mode reversed-phase/cation-exchange columns in high performance liquid chromatography (2007)
Journal Article
Davies, N. H., Euerby, M. R., & McCalley, D. V. (2007). A study of retention and overloading of basic compounds with mixed-mode reversed-phase/cation-exchange columns in high performance liquid chromatography. Journal of Chromatography A, 1138(1-2), 65-72. https://doi.org/10.1016/j.chroma.2006.10.002

The retention and overload of bases were studied on two new mixed-mode, silica based phases possessing ionic carboxylate functionalities of different acidity embedded within a hydrophobic ligand (SiELC Primesep™). At low pH, good peak shapes were obt... Read More about A study of retention and overloading of basic compounds with mixed-mode reversed-phase/cation-exchange columns in high performance liquid chromatography.

Study of overload for basic compounds in reversed-phase high performance liquid chromatography as a function of mobile phase pH (2006)
Journal Article
Davies, N. H., Euerby, M. R., & McCalley, D. V. (2006). Study of overload for basic compounds in reversed-phase high performance liquid chromatography as a function of mobile phase pH. Journal of Chromatography A, 1119(1-2), 11-19. https://doi.org/10.1016/j.chroma.2005.11.096

The retention and overloading properties for eight basic solutes and two quaternary ammonium compounds were studied over the pH range 2.7-10.0 using phosphate and carbonate buffers. At low pH, a hybrid inorganic-organic silica-ODS phase (XTerra RP-18... Read More about Study of overload for basic compounds in reversed-phase high performance liquid chromatography as a function of mobile phase pH.

Overload for ionized solutes in reversed-phase high-performance liquid chromatography (2006)
Journal Article
McCalley, D. V. (2006). Overload for ionized solutes in reversed-phase high-performance liquid chromatography. Analytical Chemistry, 78(8), 2532-2538. https://doi.org/10.1021/ac052098b

Overloading occurs for submicrogram quantities of ionized solutes particularly when using low ionic strength mobile phases at low pH (e.g., formic acid), even with highly inert silica RP-HPLC columns of normal dimensions. Much higher loads can produc... Read More about Overload for ionized solutes in reversed-phase high-performance liquid chromatography.

Study of overloading of basic drugs and peptides in reversed-phase high-performance liquid chromatography using pH adjustment of weak acid mobile phases suitable for mass spectrometry (2005)
Journal Article
McCalley, D. V. (2005). Study of overloading of basic drugs and peptides in reversed-phase high-performance liquid chromatography using pH adjustment of weak acid mobile phases suitable for mass spectrometry. Journal of Chromatography A, 1075(1-2), 57-64. https://doi.org/10.1016/j.chroma.2005.02.090

Serious losses in column efficiency for ionised basic drugs and peptides occur due to overloading of C18 phases when weak acid mobile phases of low ionic strength suitable for mass spectrometric detection are used. Measurable changes in retention tim... Read More about Study of overloading of basic drugs and peptides in reversed-phase high-performance liquid chromatography using pH adjustment of weak acid mobile phases suitable for mass spectrometry.

Comparison of an organic polymeric column and a silica-based reversed-phase for the analysis of basic peptides by high-performance liquid chromatography (2005)
Journal Article
McCalley, D. V. (2005). Comparison of an organic polymeric column and a silica-based reversed-phase for the analysis of basic peptides by high-performance liquid chromatography. Journal of Chromatography A, 1073(1-2), 137-145. https://doi.org/10.1016/j.chroma.2004.10.023

The performance of a purely polymeric and a Type B silica-based C 18 reversed-phase column was compared for the analysis of the basic peptide bradykinin and some analogues in order to assess the contribution of silanol interactions to peak shape. Goo... Read More about Comparison of an organic polymeric column and a silica-based reversed-phase for the analysis of basic peptides by high-performance liquid chromatography.

Effect of buffer on peak shape of peptides in reversed-phase high performance liquid chromatography (2004)
Journal Article
McCalley, D. V. (2004). Effect of buffer on peak shape of peptides in reversed-phase high performance liquid chromatography. Journal of Chromatography A, 1038(1-2), 77-84. https://doi.org/10.1016/j.chroma.2004.03.038

The effect of changing the buffer at constant low pH in the mobile phase is investigated with respect to the separation of a mixture of basic peptides. Considerably worse peak shapes, leading to poorer resolution of complex peptide mixtures, were obt... Read More about Effect of buffer on peak shape of peptides in reversed-phase high performance liquid chromatography.

Rationalization of retention and overloading behavior of basic compounds in reversed-phase HPLC using low ionic strength buffers suitable for mass spectrometric detection (2003)
Journal Article
McCalley, D. V. (2003). Rationalization of retention and overloading behavior of basic compounds in reversed-phase HPLC using low ionic strength buffers suitable for mass spectrometric detection. Analytical Chemistry, 75(14), 3404-3410. https://doi.org/10.1021/ac020715f

The retention and overloading behavior of some basic (and acidic) compounds has been studied on different RP-HPLC columns in buffers of varying ionic strength. Anomalous retention patterns of acids and bases were found on one phase in low-pH, volatil... Read More about Rationalization of retention and overloading behavior of basic compounds in reversed-phase HPLC using low ionic strength buffers suitable for mass spectrometric detection.

Overloading study of bases using polymeric RP-HPLC columns as an aid to rationalization of overloading on silica-ODS phases (2002)
Journal Article
Buckenmaier, S. M., McCalley, D. V., & Euerby, M. R. (2002). Overloading study of bases using polymeric RP-HPLC columns as an aid to rationalization of overloading on silica-ODS phases. Analytical Chemistry, 74(18), 4672-4681. https://doi.org/10.1021/ac0202381

The separation of ionized bases by reversed-phase liquid chromatography with alkyl silica columns often leads to severely tailed bands that are highly detrimental. Band shape and its dependence on sample mass are notably different when mobile-phase p... Read More about Overloading study of bases using polymeric RP-HPLC columns as an aid to rationalization of overloading on silica-ODS phases.

Bile metabolites of polycyclic aromatic hydrocarbons in three species of fish from the severn estuary (2002)
Journal Article
Ruddock, P. J., Bird, D. J., & McCalley, D. V. (2002). Bile metabolites of polycyclic aromatic hydrocarbons in three species of fish from the severn estuary. Ecotoxicology and Environmental Safety, 51(2), 97-105. https://doi.org/10.1006/eesa.2001.2131

Six metabolites of polycyclic aromatic hydrocarbons (PAHs) were identified and quantified from the bile of 31 common eels (Anguilla anguilla), 29 European flounders (Pleuronectes flesus), and 15 conger eels (Conger conger) collected from the Severn E... Read More about Bile metabolites of polycyclic aromatic hydrocarbons in three species of fish from the severn estuary.

Towards the development of molecularly imprinted polymer based screen-printed sensors for metabolites of PAHs (2001)
Journal Article
McCalley, D. V., Luxton, R. W., Bird, D. J., Hart, J. P., Kirsch, N., Kirsch, N., …McCalley, D. V. (2001). Towards the development of molecularly imprinted polymer based screen-printed sensors for metabolites of PAHs. Analyst, 126(11), 1936-1941. https://doi.org/10.1039/b108008n

This paper describes the fabrication of a sensor for 1-hydroxypyrene (1-OHP) based on a screen-printed carbon electrode (SPCE) modified with a molecularly imprinted polymer (MIP); 1-OHP was chosen as a model metabolite of polyaromatic hydrocarbons (P... Read More about Towards the development of molecularly imprinted polymer based screen-printed sensors for metabolites of PAHs.